IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells.

Research output: Contribution to journal › Journal article › peer-review

Standard

IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells. / Lehnen, Daniela; Barral, Serena; Cardoso, Tiago; Grealish, Shane; Heuer, Andreas; Smiyakin, Andrej; Kirkeby, Agnete; Kollet, Jutta; Cremer, Harold; Parmar, Malin; Bosio, Andreas; Knoebel, Sebastian.

In: Stem Cell Reports, Vol. 9, No. 4, 10.2017, p. 1207-1220.

Research output: Contribution to journal › Journal article › peer-review

Harvard

Lehnen, D, Barral, S, Cardoso, T, Grealish, S, Heuer, A, Smiyakin, A, Kirkeby, A, Kollet, J, Cremer, H, Parmar, M, Bosio, A & Knoebel, S 2017, 'IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells.', Stem Cell Reports, vol. 9, no. 4, pp. 1207-1220. https://doi.org/10.1016/j.stemcr.2017.08.016

APA

Lehnen, D., Barral, S., Cardoso, T., Grealish, S., Heuer, A., Smiyakin, A., Kirkeby, A., Kollet, J., Cremer, H., Parmar, M., Bosio, A., & Knoebel, S. (2017). IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells. Stem Cell Reports, 9(4), 1207-1220. https://doi.org/10.1016/j.stemcr.2017.08.016

Vancouver

Lehnen D, Barral S, Cardoso T, Grealish S, Heuer A, Smiyakin A et al. IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells. Stem Cell Reports. 2017 Oct;9(4):1207-1220. https://doi.org/10.1016/j.stemcr.2017.08.016

Author

Lehnen, Daniela ; Barral, Serena ; Cardoso, Tiago ; Grealish, Shane ; Heuer, Andreas ; Smiyakin, Andrej ; Kirkeby, Agnete ; Kollet, Jutta ; Cremer, Harold ; Parmar, Malin ; Bosio, Andreas ; Knoebel, Sebastian. / IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells. In: Stem Cell Reports. 2017 ; Vol. 9, No. 4. pp. 1207-1220.

Bibtex

@article{18381c7d937b4e68af8cd654b99d9cab,

title = "IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells.",

abstract = "Human pluripotent stem cell (hPSC)-derived mesencephalic dopaminergic (mesDA) neurons can relieve motor deficits in animal models of Parkinson's disease (PD). Clin. translation of differentiation protocols requires standardization of prodn. procedures, and surface-marker-based cell sorting is considered instrumental for reproducible generation of defined cell products. Here, we demonstrate that integrin-assocd. protein (IAP) is a cell surface marker suitable for enrichment of hPSC-derived mesDA progenitor cells. Immunomagnetically sorted IAP+ mesDA progenitors showed increased expression of ventral midbrain floor plate markers, lacked expression of pluripotency markers, and differentiated into mature dopaminergic (DA) neurons in vitro. Intrastriatal transplantation of IAP+ cells sorted at day 16 of differentiation in a rat model of PD resulted in functional recovery. Grafts from sorted IAP+ mesDA progenitors were more homogeneous in size and DA neuron d. Thus, we suggest IAP-based sorting for reproducible prospective enrichment of mesDA progenitor cells in clin. cell replacement strategies. [on SciFinder(R)]",

author = "Daniela Lehnen and Serena Barral and Tiago Cardoso and Shane Grealish and Andreas Heuer and Andrej Smiyakin and Agnete Kirkeby and Jutta Kollet and Harold Cremer and Malin Parmar and Andreas Bosio and Sebastian. Knoebel",

year = "2017",

month = oct,

doi = "10.1016/j.stemcr.2017.08.016",

language = "English",

volume = "9",

pages = "1207--1220",

journal = "Stem Cell Reports",

issn = "2213-6711",

publisher = "Cell Press",

number = "4",

}

RIS

TY - JOUR

T1 - IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells.

AU - Lehnen, Daniela

AU - Barral, Serena

AU - Cardoso, Tiago

AU - Grealish, Shane

AU - Heuer, Andreas

AU - Smiyakin, Andrej

AU - Kirkeby, Agnete

AU - Kollet, Jutta

AU - Cremer, Harold

AU - Parmar, Malin

AU - Bosio, Andreas

AU - Knoebel, Sebastian.

PY - 2017/10

Y1 - 2017/10

N2 - Human pluripotent stem cell (hPSC)-derived mesencephalic dopaminergic (mesDA) neurons can relieve motor deficits in animal models of Parkinson's disease (PD). Clin. translation of differentiation protocols requires standardization of prodn. procedures, and surface-marker-based cell sorting is considered instrumental for reproducible generation of defined cell products. Here, we demonstrate that integrin-assocd. protein (IAP) is a cell surface marker suitable for enrichment of hPSC-derived mesDA progenitor cells. Immunomagnetically sorted IAP+ mesDA progenitors showed increased expression of ventral midbrain floor plate markers, lacked expression of pluripotency markers, and differentiated into mature dopaminergic (DA) neurons in vitro. Intrastriatal transplantation of IAP+ cells sorted at day 16 of differentiation in a rat model of PD resulted in functional recovery. Grafts from sorted IAP+ mesDA progenitors were more homogeneous in size and DA neuron d. Thus, we suggest IAP-based sorting for reproducible prospective enrichment of mesDA progenitor cells in clin. cell replacement strategies. [on SciFinder(R)]

AB - Human pluripotent stem cell (hPSC)-derived mesencephalic dopaminergic (mesDA) neurons can relieve motor deficits in animal models of Parkinson's disease (PD). Clin. translation of differentiation protocols requires standardization of prodn. procedures, and surface-marker-based cell sorting is considered instrumental for reproducible generation of defined cell products. Here, we demonstrate that integrin-assocd. protein (IAP) is a cell surface marker suitable for enrichment of hPSC-derived mesDA progenitor cells. Immunomagnetically sorted IAP+ mesDA progenitors showed increased expression of ventral midbrain floor plate markers, lacked expression of pluripotency markers, and differentiated into mature dopaminergic (DA) neurons in vitro. Intrastriatal transplantation of IAP+ cells sorted at day 16 of differentiation in a rat model of PD resulted in functional recovery. Grafts from sorted IAP+ mesDA progenitors were more homogeneous in size and DA neuron d. Thus, we suggest IAP-based sorting for reproducible prospective enrichment of mesDA progenitor cells in clin. cell replacement strategies. [on SciFinder(R)]

U2 - 10.1016/j.stemcr.2017.08.016

DO - 10.1016/j.stemcr.2017.08.016

M3 - Journal article

C2 - 28943253

VL - 9

SP - 1207

EP - 1220

JO - Stem Cell Reports

JF - Stem Cell Reports

SN - 2213-6711

IS - 4

ER -

ID: 202078010