High-affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes

Research output: Contribution to journalJournal articleResearchpeer-review

  • Benjamin Lang
  • Nicolas Blot
  • Emeline Bouffartigues
  • Malcolm Buckle
  • Marcel Geertz
  • Claudio O. Gualerzi
  • Ramesh Mavathur
  • Georgi Muskhelishvili
  • Cynthia L. Pon
  • Sylvie Rimsky
  • Stella, Stefano
  • M. Madan Babu
  • Andrew Travers

The global transcriptional regulator H-NS selectively silences bacterial genes associated with pathogenicity and responses to environmental insults. Although there is ample evidence that H-NS binds preferentially to DNA containing curved regions, we show here that a major basis for this selectivity is the presence of a conserved sequence motif in H-NS target transcriptons. We further show that there is a strong tendency for the H-NS binding sites to be clustered, both within operons and in genes contained in the pathogenicity-associated islands. In accordance with previously published findings, we show that these motifs occur in AT-rich regions of DNA. On the basis of these observations, we propose that H-NS silences extensive regions of the bacterial chromosome by binding first to nucleating high-affinity sites and then spreading along AT-rich DNA. This spreading would be reinforced by the frequent occurrence of the motif in such regions. Our findings suggest that such an organization enables the silencing of extensive regions of the genetic material, thereby providing a coherent framework that unifies studies on the H-NS protein and a concrete molecular basis for the genetic control of H-NS transcriptional silencing.

Original languageEnglish
JournalNucleic Acids Research
Volume35
Issue number18
Pages (from-to)6330-6337
Number of pages8
ISSN0305-1048
DOIs
Publication statusPublished - 1 Sep 2007

ID: 202333300