Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome: a SYSDIET sub-study
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Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome : a SYSDIET sub-study. / Leder, Lena; Kolehmainen, Marjukka; Narverud, Ingunn; Dahlman, Ingrid; Myhrstad, Mari C.W.; de Mello, Vanessa; Paananen, Jussi; Carlberg, Carsten; Schwab, Ursula S; Herzig, Karl-Heinz; Cloetens, Lieselotte; Storm, Matilda Ulmius; Hukkanen, Janne; Savolainen, Markku J; Rosqvist, Fredrik; Hermansen, Kjeld; Dragsted, Lars Ove; Gunnarsdottir, Ingibjorg; Thorsdottir, Inga; Risérus, Ulf; Åkesson, Björn; Thoresen, Magne; Arner, Peter; Poutanen, Kaisa S; Uusitupa, Matti; Holven, Kirsten B; Ulven, Stine M.
In: Genes & Nutrition, Vol. 11, 3, 2016.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome
T2 - a SYSDIET sub-study
AU - Leder, Lena
AU - Kolehmainen, Marjukka
AU - Narverud, Ingunn
AU - Dahlman, Ingrid
AU - Myhrstad, Mari C.W.
AU - de Mello, Vanessa
AU - Paananen, Jussi
AU - Carlberg, Carsten
AU - Schwab, Ursula S
AU - Herzig, Karl-Heinz
AU - Cloetens, Lieselotte
AU - Storm, Matilda Ulmius
AU - Hukkanen, Janne
AU - Savolainen, Markku J
AU - Rosqvist, Fredrik
AU - Hermansen, Kjeld
AU - Dragsted, Lars Ove
AU - Gunnarsdottir, Ingibjorg
AU - Thorsdottir, Inga
AU - Risérus, Ulf
AU - Åkesson, Björn
AU - Thoresen, Magne
AU - Arner, Peter
AU - Poutanen, Kaisa S
AU - Uusitupa, Matti
AU - Holven, Kirsten B
AU - Ulven, Stine M
N1 - CURIS 2016 NEXS 207
PY - 2016
Y1 - 2016
N2 - BACKGROUND: Diet has a great impact on the risk of developing features of metabolic syndrome (MetS), type 2 diabetes mellitus (T2DM), and cardiovascular diseases (CVD). We evaluated whether a long-term healthy Nordic diet (ND) can modify the expression of inflammation and lipid metabolism-related genes in peripheral blood mononuclear cells (PBMCs) during a 2-h oral glucose tolerance test (OGTT) in individuals with MetS.METHODS: A Nordic multicenter randomized dietary study included subjects (n = 213) with MetS, randomized to a ND group or a control diet (CD) group applying an isocaloric study protocol. In this sub-study, we included subjects (n = 89) from three Nordic centers: Kuopio (n = 26), Lund (n = 30), and Oulu (n = 33) with a maximum weight change of ±4 kg, high-sensitivity C-reactive protein concentration ≤10 mg L(-1), and baseline body mass index <39 kg m(-2). PBMCs were isolated, and the mRNA gene expression analysis was measured by quantitative real-time polymerase chain reaction (qPCR). We analyzed the mRNA expression changes of 44 genes before and after a 2hOGTT at the beginning and the end of the intervention.RESULTS: The healthy ND significantly down-regulated the expression of toll-like receptor 4 (TLR4), interleukin 18 (IL18), and thrombospondin receptor (CD36) mRNA transcripts and significantly up-regulated the expression of peroxisome proliferator-activated receptor delta (PPARD) mRNA transcript after the 2hOGTT compared to the CD.CONCLUSIONS: A healthy ND is able to modify the gene expression in PBMCs after a 2hOGTT. However, more studies are needed to clarify the biological and clinical relevance of these findings.
AB - BACKGROUND: Diet has a great impact on the risk of developing features of metabolic syndrome (MetS), type 2 diabetes mellitus (T2DM), and cardiovascular diseases (CVD). We evaluated whether a long-term healthy Nordic diet (ND) can modify the expression of inflammation and lipid metabolism-related genes in peripheral blood mononuclear cells (PBMCs) during a 2-h oral glucose tolerance test (OGTT) in individuals with MetS.METHODS: A Nordic multicenter randomized dietary study included subjects (n = 213) with MetS, randomized to a ND group or a control diet (CD) group applying an isocaloric study protocol. In this sub-study, we included subjects (n = 89) from three Nordic centers: Kuopio (n = 26), Lund (n = 30), and Oulu (n = 33) with a maximum weight change of ±4 kg, high-sensitivity C-reactive protein concentration ≤10 mg L(-1), and baseline body mass index <39 kg m(-2). PBMCs were isolated, and the mRNA gene expression analysis was measured by quantitative real-time polymerase chain reaction (qPCR). We analyzed the mRNA expression changes of 44 genes before and after a 2hOGTT at the beginning and the end of the intervention.RESULTS: The healthy ND significantly down-regulated the expression of toll-like receptor 4 (TLR4), interleukin 18 (IL18), and thrombospondin receptor (CD36) mRNA transcripts and significantly up-regulated the expression of peroxisome proliferator-activated receptor delta (PPARD) mRNA transcript after the 2hOGTT compared to the CD.CONCLUSIONS: A healthy ND is able to modify the gene expression in PBMCs after a 2hOGTT. However, more studies are needed to clarify the biological and clinical relevance of these findings.
KW - Journal Article
U2 - 10.1186/s12263-016-0521-4
DO - 10.1186/s12263-016-0521-4
M3 - Journal article
C2 - 27482295
VL - 11
JO - Genes & Nutrition
JF - Genes & Nutrition
SN - 1555-8932
M1 - 3
ER -
ID: 164454475