DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death. / Bonassi, Stefano; Ceppi, Marcello; Møller, Peter; Azqueta, Amaya; Milic, Mirta; Monica, Neri; Brunborg, Gunnar; Godschalk, Roger; Koppen, Gudrun; Langie, Sabine A. S.; Teixeira, Joao Paulo; Bruzzone, Marco; Da Silva, Juliana; Benedetti, Danieli; Cavallo, Delia; Ursini, Cinzia Lucia; Giovannelli, Lisa; Moretti, Silvia; Riso, Patrizia; Del Bo', Cristian; Russo, Patrizia; Dobrzynska, Malgorzata; Goroshinskaya, Irina A.; Surikova, Ekaterina I.; Staruchova, Marta; Barancokova, Magdalena; Volkovova, Katarina; Kazimirova, Alena; Smolkova, Bozena; Laffon, Blanca; Valdiglesias, Vanessa; Pastor, Susana; Marcos, Ricard; Hernandez, Alba; Gajski, Goran; Spremo-Potparevic, Biljana; Zivkovic, Lada; Boutet-Robinet, Elisa; Perdry, Herve; Lebailly, Pierre; Perez, Carlos L.; Basaran, Nursen; Nemeth, Zsuzsanna; Safar, Anna; Dusinska, Maria; Collins, Andrew; Anderson, Diana; Anderson, Diana; Andrade, Vanessa; Pereira, Cristiana Costa; hCOMET Project.

In: Scientific Reports, Vol. 11, No. 1, 16793, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bonassi, S, Ceppi, M, Møller, P, Azqueta, A, Milic, M, Monica, N, Brunborg, G, Godschalk, R, Koppen, G, Langie, SAS, Teixeira, JP, Bruzzone, M, Da Silva, J, Benedetti, D, Cavallo, D, Ursini, CL, Giovannelli, L, Moretti, S, Riso, P, Del Bo', C, Russo, P, Dobrzynska, M, Goroshinskaya, IA, Surikova, EI, Staruchova, M, Barancokova, M, Volkovova, K, Kazimirova, A, Smolkova, B, Laffon, B, Valdiglesias, V, Pastor, S, Marcos, R, Hernandez, A, Gajski, G, Spremo-Potparevic, B, Zivkovic, L, Boutet-Robinet, E, Perdry, H, Lebailly, P, Perez, CL, Basaran, N, Nemeth, Z, Safar, A, Dusinska, M, Collins, A, Anderson, D, Anderson, D, Andrade, V, Pereira, CC & hCOMET Project 2021, 'DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death', Scientific Reports, vol. 11, no. 1, 16793. https://doi.org/10.1038/s41598-021-95976-7

APA

Bonassi, S., Ceppi, M., Møller, P., Azqueta, A., Milic, M., Monica, N., Brunborg, G., Godschalk, R., Koppen, G., Langie, S. A. S., Teixeira, J. P., Bruzzone, M., Da Silva, J., Benedetti, D., Cavallo, D., Ursini, C. L., Giovannelli, L., Moretti, S., Riso, P., ... hCOMET Project (2021). DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death. Scientific Reports, 11(1), [16793]. https://doi.org/10.1038/s41598-021-95976-7

Vancouver

Bonassi S, Ceppi M, Møller P, Azqueta A, Milic M, Monica N et al. DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death. Scientific Reports. 2021;11(1). 16793. https://doi.org/10.1038/s41598-021-95976-7

Author

Bonassi, Stefano ; Ceppi, Marcello ; Møller, Peter ; Azqueta, Amaya ; Milic, Mirta ; Monica, Neri ; Brunborg, Gunnar ; Godschalk, Roger ; Koppen, Gudrun ; Langie, Sabine A. S. ; Teixeira, Joao Paulo ; Bruzzone, Marco ; Da Silva, Juliana ; Benedetti, Danieli ; Cavallo, Delia ; Ursini, Cinzia Lucia ; Giovannelli, Lisa ; Moretti, Silvia ; Riso, Patrizia ; Del Bo', Cristian ; Russo, Patrizia ; Dobrzynska, Malgorzata ; Goroshinskaya, Irina A. ; Surikova, Ekaterina I. ; Staruchova, Marta ; Barancokova, Magdalena ; Volkovova, Katarina ; Kazimirova, Alena ; Smolkova, Bozena ; Laffon, Blanca ; Valdiglesias, Vanessa ; Pastor, Susana ; Marcos, Ricard ; Hernandez, Alba ; Gajski, Goran ; Spremo-Potparevic, Biljana ; Zivkovic, Lada ; Boutet-Robinet, Elisa ; Perdry, Herve ; Lebailly, Pierre ; Perez, Carlos L. ; Basaran, Nursen ; Nemeth, Zsuzsanna ; Safar, Anna ; Dusinska, Maria ; Collins, Andrew ; Anderson, Diana ; Anderson, Diana ; Andrade, Vanessa ; Pereira, Cristiana Costa ; hCOMET Project. / DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death. In: Scientific Reports. 2021 ; Vol. 11, No. 1.

Bibtex

@article{bf3922c06a614087960daffeac9f8177,

title = "DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death",

abstract = "The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan-Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06-1.90) for overall mortality, and 1.94 (1.04-3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.",

keywords = "CHROMOSOMAL-ABERRATIONS, HUMAN CANCER, LYMPHOCYTES PREDICTS, FREQUENCY, VALIDATION, BIOMARKERS, HALLMARKS, ADDUCTS, REPAIR, COHORT",

author = "Stefano Bonassi and Marcello Ceppi and Peter M{\o}ller and Amaya Azqueta and Mirta Milic and Neri Monica and Gunnar Brunborg and Roger Godschalk and Gudrun Koppen and Langie, {Sabine A. S.} and Teixeira, {Joao Paulo} and Marco Bruzzone and {Da Silva}, Juliana and Danieli Benedetti and Delia Cavallo and Ursini, {Cinzia Lucia} and Lisa Giovannelli and Silvia Moretti and Patrizia Riso and {Del Bo'}, Cristian and Patrizia Russo and Malgorzata Dobrzynska and Goroshinskaya, {Irina A.} and Surikova, {Ekaterina I.} and Marta Staruchova and Magdalena Barancokova and Katarina Volkovova and Alena Kazimirova and Bozena Smolkova and Blanca Laffon and Vanessa Valdiglesias and Susana Pastor and Ricard Marcos and Alba Hernandez and Goran Gajski and Biljana Spremo-Potparevic and Lada Zivkovic and Elisa Boutet-Robinet and Herve Perdry and Pierre Lebailly and Perez, {Carlos L.} and Nursen Basaran and Zsuzsanna Nemeth and Anna Safar and Maria Dusinska and Andrew Collins and Diana Anderson and Diana Anderson and Vanessa Andrade and Pereira, {Cristiana Costa} and {hCOMET Project}",

year = "2021",

doi = "10.1038/s41598-021-95976-7",

language = "English",

volume = "11",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "nature publishing group",

number = "1",

}

RIS

TY - JOUR

T1 - DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

AU - Bonassi, Stefano

AU - Ceppi, Marcello

AU - Møller, Peter

AU - Azqueta, Amaya

AU - Milic, Mirta

AU - Monica, Neri

AU - Brunborg, Gunnar

AU - Godschalk, Roger

AU - Koppen, Gudrun

AU - Langie, Sabine A. S.

AU - Teixeira, Joao Paulo

AU - Bruzzone, Marco

AU - Da Silva, Juliana

AU - Benedetti, Danieli

AU - Cavallo, Delia

AU - Ursini, Cinzia Lucia

AU - Giovannelli, Lisa

AU - Moretti, Silvia

AU - Riso, Patrizia

AU - Del Bo', Cristian

AU - Russo, Patrizia

AU - Dobrzynska, Malgorzata

AU - Goroshinskaya, Irina A.

AU - Surikova, Ekaterina I.

AU - Staruchova, Marta

AU - Barancokova, Magdalena

AU - Volkovova, Katarina

AU - Kazimirova, Alena

AU - Smolkova, Bozena

AU - Laffon, Blanca

AU - Valdiglesias, Vanessa

AU - Pastor, Susana

AU - Marcos, Ricard

AU - Hernandez, Alba

AU - Gajski, Goran

AU - Spremo-Potparevic, Biljana

AU - Zivkovic, Lada

AU - Boutet-Robinet, Elisa

AU - Perdry, Herve

AU - Lebailly, Pierre

AU - Perez, Carlos L.

AU - Basaran, Nursen

AU - Nemeth, Zsuzsanna

AU - Safar, Anna

AU - Dusinska, Maria

AU - Collins, Andrew

AU - Anderson, Diana

AU - Andrade, Vanessa

AU - Pereira, Cristiana Costa

AU - hCOMET Project

PY - 2021

Y1 - 2021

N2 - The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan-Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06-1.90) for overall mortality, and 1.94 (1.04-3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.

AB - The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan-Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06-1.90) for overall mortality, and 1.94 (1.04-3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.

KW - CHROMOSOMAL-ABERRATIONS

KW - HUMAN CANCER

KW - LYMPHOCYTES PREDICTS

KW - FREQUENCY

KW - VALIDATION

KW - BIOMARKERS

KW - HALLMARKS

KW - ADDUCTS

KW - REPAIR

KW - COHORT

U2 - 10.1038/s41598-021-95976-7

DO - 10.1038/s41598-021-95976-7

M3 - Journal article

C2 - 34408182

VL - 11

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 16793

ER -

ID: 277646974