Dietary modulation of the gut microbiota - a randomised controlled trial in obese postmenopausal women
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Dietary modulation of the gut microbiota - a randomised controlled trial in obese postmenopausal women. / Brahe, Lena Kirchner; Le Chatelier, Emmanuelle; Prifti, Edi; Pons, Nicolas; Kennedy, Sean; Blædel, Trine; Håkansson, Janet; Dalsgaard, Trine Kastrup; Hansen, Torben; Pedersen, Oluf Borbye; Astrup, Arne; Ehrlich, S Dusko; Larsen, Lesli Hingstrup.
In: British Journal of Nutrition, Vol. 114, No. 3, 2015, p. 406-417.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Dietary modulation of the gut microbiota - a randomised controlled trial in obese postmenopausal women
AU - Brahe, Lena Kirchner
AU - Le Chatelier, Emmanuelle
AU - Prifti, Edi
AU - Pons, Nicolas
AU - Kennedy, Sean
AU - Blædel, Trine
AU - Håkansson, Janet
AU - Dalsgaard, Trine Kastrup
AU - Hansen, Torben
AU - Pedersen, Oluf Borbye
AU - Astrup, Arne
AU - Ehrlich, S Dusko
AU - Larsen, Lesli Hingstrup
N1 - CURIS 2015 NEXS 241
PY - 2015
Y1 - 2015
N2 - The gut microbiota has been implicated in obesity and its progression towards metabolic disease. Dietary interventions that target the gut microbiota have been suggested to improve metabolic health. The aim of the present study was to investigate the effect of interventions with Lactobacillus paracasei F19 or flaxseed mucilage on the gut microbiota and metabolic risk markers in obesity. A total of fifty-eight obese postmenopausal women were randomised to a single-blinded, parallel-group intervention of 6-week duration, with a daily intake of either L. paracasei F19 (9·4 × 1010 colony-forming units), flaxseed mucilage (10 g) or placebo. Quantitative metagenomic analysis of faecal DNA was performed to identify the changes in the gut microbiota. Diet-induced changes in metabolic markers were explored using adjusted linear regression models. The intake of flaxseed mucilage over 6 weeks led to a reduction in serum C-peptide and insulin release during an oral glucose tolerance test (P< 0·05) and improved insulin sensitivity measured by Matsuda index (P< 0·05). Comparison of gut microbiota composition at baseline and after 6 weeks of intervention with flaxseed mucilage showed alterations in abundance of thirty-three metagenomic species (P< 0·01), including decreased relative abundance of eight Faecalibacterium species. These changes in the microbiota could not explain the effect of flaxseed mucilage on insulin sensitivity. The intake of L. paracasei F19 did not modulate metabolic markers compared with placebo. In conclusion, flaxseed mucilage improves insulin sensitivity and alters the gut microbiota; however, the improvement in insulin sensitivity was not mediated by the observed changes in relative abundance of bacterial species.
AB - The gut microbiota has been implicated in obesity and its progression towards metabolic disease. Dietary interventions that target the gut microbiota have been suggested to improve metabolic health. The aim of the present study was to investigate the effect of interventions with Lactobacillus paracasei F19 or flaxseed mucilage on the gut microbiota and metabolic risk markers in obesity. A total of fifty-eight obese postmenopausal women were randomised to a single-blinded, parallel-group intervention of 6-week duration, with a daily intake of either L. paracasei F19 (9·4 × 1010 colony-forming units), flaxseed mucilage (10 g) or placebo. Quantitative metagenomic analysis of faecal DNA was performed to identify the changes in the gut microbiota. Diet-induced changes in metabolic markers were explored using adjusted linear regression models. The intake of flaxseed mucilage over 6 weeks led to a reduction in serum C-peptide and insulin release during an oral glucose tolerance test (P< 0·05) and improved insulin sensitivity measured by Matsuda index (P< 0·05). Comparison of gut microbiota composition at baseline and after 6 weeks of intervention with flaxseed mucilage showed alterations in abundance of thirty-three metagenomic species (P< 0·01), including decreased relative abundance of eight Faecalibacterium species. These changes in the microbiota could not explain the effect of flaxseed mucilage on insulin sensitivity. The intake of L. paracasei F19 did not modulate metabolic markers compared with placebo. In conclusion, flaxseed mucilage improves insulin sensitivity and alters the gut microbiota; however, the improvement in insulin sensitivity was not mediated by the observed changes in relative abundance of bacterial species.
U2 - 10.1017/S0007114515001786
DO - 10.1017/S0007114515001786
M3 - Journal article
C2 - 26134388
VL - 114
SP - 406
EP - 417
JO - British Journal of Nutrition
JF - British Journal of Nutrition
SN - 0007-1145
IS - 3
ER -
ID: 141015888