Coordinated replication and sequestration of oriC and dnaA are required for maintaining controlled once-per-cell-cycle initiation in Escherichia coli

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Coordinated replication and sequestration of oriC and dnaA are required for maintaining controlled once-per-cell-cycle initiation in Escherichia coli. / Riber, Leise; Løbner-Olesen, Anders.

In: Journal of Bacteriology, Vol. 187, No. 16, 01.08.2005, p. 5605-5613.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Riber, L & Løbner-Olesen, A 2005, 'Coordinated replication and sequestration of oriC and dnaA are required for maintaining controlled once-per-cell-cycle initiation in Escherichia coli', Journal of Bacteriology, vol. 187, no. 16, pp. 5605-5613. https://doi.org/10.1128/JB.187.16.5605-5613.2005

APA

Riber, L., & Løbner-Olesen, A. (2005). Coordinated replication and sequestration of oriC and dnaA are required for maintaining controlled once-per-cell-cycle initiation in Escherichia coli. Journal of Bacteriology, 187(16), 5605-5613. https://doi.org/10.1128/JB.187.16.5605-5613.2005

Vancouver

Riber L, Løbner-Olesen A. Coordinated replication and sequestration of oriC and dnaA are required for maintaining controlled once-per-cell-cycle initiation in Escherichia coli. Journal of Bacteriology. 2005 Aug 1;187(16):5605-5613. https://doi.org/10.1128/JB.187.16.5605-5613.2005

Author

Riber, Leise ; Løbner-Olesen, Anders. / Coordinated replication and sequestration of oriC and dnaA are required for maintaining controlled once-per-cell-cycle initiation in Escherichia coli. In: Journal of Bacteriology. 2005 ; Vol. 187, No. 16. pp. 5605-5613.

Bibtex

@article{a9c08eb0f05a444aa28e400ba92c5bca,
title = "Coordinated replication and sequestration of oriC and dnaA are required for maintaining controlled once-per-cell-cycle initiation in Escherichia coli",
abstract = "Escherichia coli cells were constructed in which the dnaA gene was moved to a location opposite oriC on the circular chromosome. In these cells the dnaA gene was replicated with significant delay relative to the origin. Consequently, the period where the newly replicated and hemimethylated oriC was sequestered no longer coincided with the period where the dnaA gene promoter was sequestered. DnaA protein synthesis was therefore expected to continue during origin sequestration. Despite a normal length of the sequestration period in such cells, they had increased origin content and also displayed asynchrony of initiation. This indicated that reinitiation occasionally occurred at some origins within the same cell cycle. The extra initiations took place in spite of a reduction in total DnaA protein concentration to about half of the wild-type level. We propose that this more efficient utilization of DnaA protein results from an increased availability at the end of the origin sequestration period. Therefore, coordinated sequestration of oriC and dnaA is required for maintaining controlled once-per-cell-cycle initiation.",
author = "Leise Riber and Anders L{\o}bner-Olesen",
year = "2005",
month = aug,
day = "1",
doi = "10.1128/JB.187.16.5605-5613.2005",
language = "English",
volume = "187",
pages = "5605--5613",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "16",

}

RIS

TY - JOUR

T1 - Coordinated replication and sequestration of oriC and dnaA are required for maintaining controlled once-per-cell-cycle initiation in Escherichia coli

AU - Riber, Leise

AU - Løbner-Olesen, Anders

PY - 2005/8/1

Y1 - 2005/8/1

N2 - Escherichia coli cells were constructed in which the dnaA gene was moved to a location opposite oriC on the circular chromosome. In these cells the dnaA gene was replicated with significant delay relative to the origin. Consequently, the period where the newly replicated and hemimethylated oriC was sequestered no longer coincided with the period where the dnaA gene promoter was sequestered. DnaA protein synthesis was therefore expected to continue during origin sequestration. Despite a normal length of the sequestration period in such cells, they had increased origin content and also displayed asynchrony of initiation. This indicated that reinitiation occasionally occurred at some origins within the same cell cycle. The extra initiations took place in spite of a reduction in total DnaA protein concentration to about half of the wild-type level. We propose that this more efficient utilization of DnaA protein results from an increased availability at the end of the origin sequestration period. Therefore, coordinated sequestration of oriC and dnaA is required for maintaining controlled once-per-cell-cycle initiation.

AB - Escherichia coli cells were constructed in which the dnaA gene was moved to a location opposite oriC on the circular chromosome. In these cells the dnaA gene was replicated with significant delay relative to the origin. Consequently, the period where the newly replicated and hemimethylated oriC was sequestered no longer coincided with the period where the dnaA gene promoter was sequestered. DnaA protein synthesis was therefore expected to continue during origin sequestration. Despite a normal length of the sequestration period in such cells, they had increased origin content and also displayed asynchrony of initiation. This indicated that reinitiation occasionally occurred at some origins within the same cell cycle. The extra initiations took place in spite of a reduction in total DnaA protein concentration to about half of the wild-type level. We propose that this more efficient utilization of DnaA protein results from an increased availability at the end of the origin sequestration period. Therefore, coordinated sequestration of oriC and dnaA is required for maintaining controlled once-per-cell-cycle initiation.

U2 - 10.1128/JB.187.16.5605-5613.2005

DO - 10.1128/JB.187.16.5605-5613.2005

M3 - Journal article

C2 - 16077105

VL - 187

SP - 5605

EP - 5613

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 16

ER -

ID: 33971683