Automobile diesel exhaust particles induce lipid droplet formation in macrophages in vitro

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Automobile diesel exhaust particles induce lipid droplet formation in macrophages in vitro. / Cao, Yi; Jantzen, Kim; Gouveia, Ana Cecilia Damiao; Skovmand, Astrid; Roursgaard, Martin; Loft, Steffen; Møller, Peter.

In: Environmental Toxicology and Pharmacology, Vol. 40, No. 1, 09.06.2015, p. 164-171.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Cao, Y, Jantzen, K, Gouveia, ACD, Skovmand, A, Roursgaard, M, Loft, S & Møller, P 2015, 'Automobile diesel exhaust particles induce lipid droplet formation in macrophages in vitro', Environmental Toxicology and Pharmacology, vol. 40, no. 1, pp. 164-171. https://doi.org/10.1016/j.etap.2015.06.012

APA

Cao, Y., Jantzen, K., Gouveia, A. C. D., Skovmand, A., Roursgaard, M., Loft, S., & Møller, P. (2015). Automobile diesel exhaust particles induce lipid droplet formation in macrophages in vitro. Environmental Toxicology and Pharmacology, 40(1), 164-171. https://doi.org/10.1016/j.etap.2015.06.012

Vancouver

Cao Y, Jantzen K, Gouveia ACD, Skovmand A, Roursgaard M, Loft S et al. Automobile diesel exhaust particles induce lipid droplet formation in macrophages in vitro. Environmental Toxicology and Pharmacology. 2015 Jun 9;40(1):164-171. https://doi.org/10.1016/j.etap.2015.06.012

Author

Cao, Yi ; Jantzen, Kim ; Gouveia, Ana Cecilia Damiao ; Skovmand, Astrid ; Roursgaard, Martin ; Loft, Steffen ; Møller, Peter. / Automobile diesel exhaust particles induce lipid droplet formation in macrophages in vitro. In: Environmental Toxicology and Pharmacology. 2015 ; Vol. 40, No. 1. pp. 164-171.

Bibtex

@article{3f2536dbe2494b639bb81310a547df5a,

title = "Automobile diesel exhaust particles induce lipid droplet formation in macrophages in vitro",

abstract = "Exposure to diesel exhaust particles (DEP) has been associated with adverse cardiopulmonary health effects, which may be related to dysregulation of lipid metabolism and formation of macrophage foam cells. In this study, THP-1 derived macrophages were exposed to an automobile generated DEP (A-DEP) for 24h to study lipid droplet formation and possible mechanisms. The results show that A-DEP did not induce cytotoxicity. The production of reactive oxygen species was only significantly increased after exposure for 3h, but not 24h. Intracellular level of reduced glutathione was increased after 24h exposure. These results combined indicate an adaptive response to oxidative stress. Exposure to A-DEP was associated with significantly increased formation of lipid droplets, as well as changes in lysosomal function, assessed as reduced LysoTracker staining. In conclusion, these results indicated that exposure to A-DEP may induce formation of lipid droplets in macrophages in vitro possibly via lysosomal dysfunction.",

author = "Yi Cao and Kim Jantzen and Gouveia, {Ana Cecilia Damiao} and Astrid Skovmand and Martin Roursgaard and Steffen Loft and Peter M{\o}ller",

year = "2015",

month = jun,

day = "9",

doi = "10.1016/j.etap.2015.06.012",

language = "English",

volume = "40",

pages = "164--171",

journal = "Environmental Toxicology and Pharmacology",

issn = "1382-6689",

publisher = "Elsevier",

number = "1",

}

RIS

TY - JOUR

T1 - Automobile diesel exhaust particles induce lipid droplet formation in macrophages in vitro

AU - Cao, Yi

AU - Jantzen, Kim

AU - Gouveia, Ana Cecilia Damiao

AU - Skovmand, Astrid

AU - Roursgaard, Martin

AU - Loft, Steffen

AU - Møller, Peter

PY - 2015/6/9

Y1 - 2015/6/9

N2 - Exposure to diesel exhaust particles (DEP) has been associated with adverse cardiopulmonary health effects, which may be related to dysregulation of lipid metabolism and formation of macrophage foam cells. In this study, THP-1 derived macrophages were exposed to an automobile generated DEP (A-DEP) for 24h to study lipid droplet formation and possible mechanisms. The results show that A-DEP did not induce cytotoxicity. The production of reactive oxygen species was only significantly increased after exposure for 3h, but not 24h. Intracellular level of reduced glutathione was increased after 24h exposure. These results combined indicate an adaptive response to oxidative stress. Exposure to A-DEP was associated with significantly increased formation of lipid droplets, as well as changes in lysosomal function, assessed as reduced LysoTracker staining. In conclusion, these results indicated that exposure to A-DEP may induce formation of lipid droplets in macrophages in vitro possibly via lysosomal dysfunction.

AB - Exposure to diesel exhaust particles (DEP) has been associated with adverse cardiopulmonary health effects, which may be related to dysregulation of lipid metabolism and formation of macrophage foam cells. In this study, THP-1 derived macrophages were exposed to an automobile generated DEP (A-DEP) for 24h to study lipid droplet formation and possible mechanisms. The results show that A-DEP did not induce cytotoxicity. The production of reactive oxygen species was only significantly increased after exposure for 3h, but not 24h. Intracellular level of reduced glutathione was increased after 24h exposure. These results combined indicate an adaptive response to oxidative stress. Exposure to A-DEP was associated with significantly increased formation of lipid droplets, as well as changes in lysosomal function, assessed as reduced LysoTracker staining. In conclusion, these results indicated that exposure to A-DEP may induce formation of lipid droplets in macrophages in vitro possibly via lysosomal dysfunction.

U2 - 10.1016/j.etap.2015.06.012

DO - 10.1016/j.etap.2015.06.012

M3 - Journal article

C2 - 26122084

VL - 40

SP - 164

EP - 171

JO - Environmental Toxicology and Pharmacology

JF - Environmental Toxicology and Pharmacology

SN - 1382-6689

IS - 1

ER -

ID: 140726427