Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies

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Clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes, a diverse family of prokaryotic adaptive immune systems, have emerged as a biotechnological tool and therapeutic. The discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPR (Acr) proteins, enables the development of more controllable and precise CRISPR-Cas tools. Here we discuss applications of Acr proteins for post-translational control of CRISPR-Cas systems in prokaryotic and mammalian cells, organisms and ecosystems.

Original languageEnglish
JournalNature Methods
Issue number5
Pages (from-to)471-479
Number of pages9
Publication statusPublished - 2020

ID: 239855966