Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells
Research output: Contribution to journal › Journal article › Research › peer-review
AIM: The current study was designed to characterize the anticancer effects of clotrimazole on human cutaneous melanoma cells.
MATERIALS AND METHODS: The v-raf murine sarcoma viral oncogene homolog B1 V600E mutant melanoma cell line A375 was used as an in vitro model. Characterization tools included analyses of cell viability, gene expression, cell-cycle progression, annexin V reactivity and internucleosomal DNA fragmentation.
RESULTS: Clotrimazole induced cytotoxicity in A375 human melanoma cells without significant changes of human keratinocyte cell viability. Clotrimazole, at a concentration that approximates the inhibitory concentration 50% (IC50) value (i.e. 10 μM), reduced the expression of hexokinase type-II, induced cell-cycle arrest at G1-S phase transition, altered annexin V reactivity and induced DNA fragmentation without evidence of necrosis.
CONCLUSION: The current study provides evidence of a remarkable pro-apoptotic effect by clotrimazole against human melanoma cells, with a different mechanism of action and timeline of the apoptosis-related events when compared to cisplatin.
|Number of pages||6|
|Publication status||Published - Jul 2015|
- Annexin A5, Antineoplastic Agents, Apoptosis, Cell Line, Tumor, Cell Survival, Cisplatin, Clotrimazole, DNA Fragmentation, G1 Phase Cell Cycle Checkpoints, Humans, Keratinocytes, Melanoma