Xenopus oocyte electrophysiology in GPCR drug discovery

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research

Standard

Xenopus oocyte electrophysiology in GPCR drug discovery. / Hansen, Kasper Bø; Bräuner-Osborne, Hans.

G protein-coupled receptors in drug discovery. ed. / Wayne R Leifert. Vol. 552 United States : Humana Press, 2009. p. 343-357 (Methods in Molecular Biology, Vol. 552).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research

Harvard

Hansen, KB & Bräuner-Osborne, H 2009, Xenopus oocyte electrophysiology in GPCR drug discovery. in WR Leifert (ed.), G protein-coupled receptors in drug discovery. vol. 552, Humana Press, United States, Methods in Molecular Biology, vol. 552, pp. 343-357. https://doi.org/10.1007/978-1-60327-317-6_25

APA

Hansen, K. B., & Bräuner-Osborne, H. (2009). Xenopus oocyte electrophysiology in GPCR drug discovery. In W. R. Leifert (Ed.), G protein-coupled receptors in drug discovery (Vol. 552, pp. 343-357). Humana Press. Methods in Molecular Biology Vol. 552 https://doi.org/10.1007/978-1-60327-317-6_25

Vancouver

Hansen KB, Bräuner-Osborne H. Xenopus oocyte electrophysiology in GPCR drug discovery. In Leifert WR, editor, G protein-coupled receptors in drug discovery. Vol. 552. United States: Humana Press. 2009. p. 343-357. (Methods in Molecular Biology, Vol. 552). https://doi.org/10.1007/978-1-60327-317-6_25

Author

Hansen, Kasper Bø ; Bräuner-Osborne, Hans. / Xenopus oocyte electrophysiology in GPCR drug discovery. G protein-coupled receptors in drug discovery. editor / Wayne R Leifert. Vol. 552 United States : Humana Press, 2009. pp. 343-357 (Methods in Molecular Biology, Vol. 552).

Bibtex

@inbook{f35cc8406c6e11de8bc9000ea68e967b,
title = "Xenopus oocyte electrophysiology in GPCR drug discovery",
abstract = "Deorphanization of the large group of G protein-coupled receptors (GPCRs) for which an endogenous activating ligand has not yet been identified (orphan GPCRs) has become increasingly difficult. A specialized technique that has been successfully applied to deorphanize some of these GPCRs involves two-electrode voltage-clamp recordings of currents through ion channels, which are activated by GPCRs heterologously expressed in Xenopus oocytes. The ion channels that couple to GPCR activation in Xenopus oocytes can be endogenous calcium-activated chloride channels (CaCCs) or heterologously expressed G protein-coupled inwardly rectifying potassium channels (GIRKs). We will describe a general approach for expression of GPCRs in Xenopus oocytes and characterization of these using electrophysiological recordings. We will focus on the detection of GPCR activation by recordings of currents through CaCCs that are activated by calcium release from the endoplasmic reticulum and thus the G(q) signaling pathway.",
keywords = "Former Faculty of Pharmaceutical Sciences",
author = "Hansen, {Kasper B{\o}} and Hans Br{\"a}uner-Osborne",
note = "Keywords: Xenopus laevis, Oocyte, RNA, Transcription, Injection, GPRC6A, Intracellular calcium, Ligand. Chapter 25",
year = "2009",
doi = "10.1007/978-1-60327-317-6_25",
language = "English",
isbn = "978-1-60327-316-9",
volume = "552",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "343--357",
editor = "Leifert, {Wayne R}",
booktitle = "G protein-coupled receptors in drug discovery",
address = "United States",

}

RIS

TY - CHAP

T1 - Xenopus oocyte electrophysiology in GPCR drug discovery

AU - Hansen, Kasper Bø

AU - Bräuner-Osborne, Hans

N1 - Keywords: Xenopus laevis, Oocyte, RNA, Transcription, Injection, GPRC6A, Intracellular calcium, Ligand. Chapter 25

PY - 2009

Y1 - 2009

N2 - Deorphanization of the large group of G protein-coupled receptors (GPCRs) for which an endogenous activating ligand has not yet been identified (orphan GPCRs) has become increasingly difficult. A specialized technique that has been successfully applied to deorphanize some of these GPCRs involves two-electrode voltage-clamp recordings of currents through ion channels, which are activated by GPCRs heterologously expressed in Xenopus oocytes. The ion channels that couple to GPCR activation in Xenopus oocytes can be endogenous calcium-activated chloride channels (CaCCs) or heterologously expressed G protein-coupled inwardly rectifying potassium channels (GIRKs). We will describe a general approach for expression of GPCRs in Xenopus oocytes and characterization of these using electrophysiological recordings. We will focus on the detection of GPCR activation by recordings of currents through CaCCs that are activated by calcium release from the endoplasmic reticulum and thus the G(q) signaling pathway.

AB - Deorphanization of the large group of G protein-coupled receptors (GPCRs) for which an endogenous activating ligand has not yet been identified (orphan GPCRs) has become increasingly difficult. A specialized technique that has been successfully applied to deorphanize some of these GPCRs involves two-electrode voltage-clamp recordings of currents through ion channels, which are activated by GPCRs heterologously expressed in Xenopus oocytes. The ion channels that couple to GPCR activation in Xenopus oocytes can be endogenous calcium-activated chloride channels (CaCCs) or heterologously expressed G protein-coupled inwardly rectifying potassium channels (GIRKs). We will describe a general approach for expression of GPCRs in Xenopus oocytes and characterization of these using electrophysiological recordings. We will focus on the detection of GPCR activation by recordings of currents through CaCCs that are activated by calcium release from the endoplasmic reticulum and thus the G(q) signaling pathway.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1007/978-1-60327-317-6_25

DO - 10.1007/978-1-60327-317-6_25

M3 - Book chapter

C2 - 19513662

SN - 978-1-60327-316-9

VL - 552

T3 - Methods in Molecular Biology

SP - 343

EP - 357

BT - G protein-coupled receptors in drug discovery

A2 - Leifert, Wayne R

PB - Humana Press

CY - United States

ER -

ID: 13063250