Virulent coliphages in 1-year-old children fecal samples are fewer, but more infectious than temperate coliphages
Research output: Contribution to journal › Journal article › peer-review
Standard
Virulent coliphages in 1-year-old children fecal samples are fewer, but more infectious than temperate coliphages. / Mathieu, Aurélie; Dion, Moïra; Deng, Ling; Tremblay, Denise; Moncaut, Elisabeth; Shah, Shiraz A.; Stokholm, Jakob; Krogfelt, Karen A.; Schjørring, Susanne; Bisgaard, Hans; Nielsen, Dennis S.; Moineau, Sylvain; Petit, Marie-Agnès.
In: Nature Communications, Vol. 11, 378, 2020.Research output: Contribution to journal › Journal article › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Virulent coliphages in 1-year-old children fecal samples are fewer, but more infectious than temperate coliphages
AU - Mathieu, Aurélie
AU - Dion, Moïra
AU - Deng, Ling
AU - Tremblay, Denise
AU - Moncaut, Elisabeth
AU - Shah, Shiraz A.
AU - Stokholm, Jakob
AU - Krogfelt, Karen A.
AU - Schjørring, Susanne
AU - Bisgaard, Hans
AU - Nielsen, Dennis S.
AU - Moineau, Sylvain
AU - Petit, Marie-Agnès
PY - 2020
Y1 - 2020
N2 - Bacteriophages constitute an important part of the human gut microbiota, but their impact on this community is largely unknown. Here, we cultivate temperate phages produced by 900 E. coli strains isolated from 648 fecal samples from 1-year-old children and obtain coliphages directly from the viral fraction of the same fecal samples. We find that 63% of strains hosted phages, while 24% of the viromes contain phages targeting E. coli. 150 of these phages, half recovered from strain supernatants, half from virome (73% temperate and 27% virulent) were tested for their host range on 75 E. coli strains isolated from the same cohort. Temperate phages barely infected the gut strains, whereas virulent phages killed up to 68% of them. We conclude that in fecal samples from children, temperate coliphages dominate, while virulent ones have greater infectivity and broader host range, likely playing a role in gut microbiota dynamics.
AB - Bacteriophages constitute an important part of the human gut microbiota, but their impact on this community is largely unknown. Here, we cultivate temperate phages produced by 900 E. coli strains isolated from 648 fecal samples from 1-year-old children and obtain coliphages directly from the viral fraction of the same fecal samples. We find that 63% of strains hosted phages, while 24% of the viromes contain phages targeting E. coli. 150 of these phages, half recovered from strain supernatants, half from virome (73% temperate and 27% virulent) were tested for their host range on 75 E. coli strains isolated from the same cohort. Temperate phages barely infected the gut strains, whereas virulent phages killed up to 68% of them. We conclude that in fecal samples from children, temperate coliphages dominate, while virulent ones have greater infectivity and broader host range, likely playing a role in gut microbiota dynamics.
U2 - 10.1038/s41467-019-14042-z
DO - 10.1038/s41467-019-14042-z
M3 - Journal article
C2 - 31953385
AN - SCOPUS:85078017316
VL - 11
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
M1 - 378
ER -
ID: 235920013