Unravelling the ultrastructure of ascending colon fluids from patients with ulcerative colitis by cryogenic transmission electron microscopy
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Unravelling the ultrastructure of ascending colon fluids from patients with ulcerative colitis by cryogenic transmission electron microscopy. / Müllertz, Anette; Fatouros, Dimitrios G; Vertzoni, Maria; Reppas, Christos.
In: The Journal of pharmacy and pharmacology, Vol. 65, No. 10, 10.2013, p. 1482-7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Unravelling the ultrastructure of ascending colon fluids from patients with ulcerative colitis by cryogenic transmission electron microscopy
AU - Müllertz, Anette
AU - Fatouros, Dimitrios G
AU - Vertzoni, Maria
AU - Reppas, Christos
N1 - © 2013 Royal Pharmaceutical Society.
PY - 2013/10
Y1 - 2013/10
N2 - OBJECTIVES: Studies on the characterization of ascending colonic fluids are scarce, limited to physicochemical characterization of their composition, and little is known for the morphology of the produced colloidal phases. In an attempt to gain insights for their structure at the ultrastructural level, samples from the lumen of ascending colon were collected from patients with ulcerative colitis in remission.METHODS: After ultracentrifugation, the supernatants of two samples (one with high and one with low cholesterol level) were visualized by means of cryogenic transmission electron microscopy.KEY FINDINGS: In the supernatants with high cholesterol content, micellar-like structures, bilayer fragments, open vesicles, and uni-, bi- and trilamellar vesicles were abundant. In addition, crystals of cholesterol were frequently observed. In contrast, in the sample with low cholesterol content, oily solids, plates of cholesterol monohydrate and elongated structures were present. Few unilamellar vesicles were occasionally visualized.CONCLUSIONS: The current study gives direct evidence, for the first time, of the existence of 'remnants' of lipolytic products in the fasted ascending colon. The impact of these structures to colonic absorption of drugs is an open question.
AB - OBJECTIVES: Studies on the characterization of ascending colonic fluids are scarce, limited to physicochemical characterization of their composition, and little is known for the morphology of the produced colloidal phases. In an attempt to gain insights for their structure at the ultrastructural level, samples from the lumen of ascending colon were collected from patients with ulcerative colitis in remission.METHODS: After ultracentrifugation, the supernatants of two samples (one with high and one with low cholesterol level) were visualized by means of cryogenic transmission electron microscopy.KEY FINDINGS: In the supernatants with high cholesterol content, micellar-like structures, bilayer fragments, open vesicles, and uni-, bi- and trilamellar vesicles were abundant. In addition, crystals of cholesterol were frequently observed. In contrast, in the sample with low cholesterol content, oily solids, plates of cholesterol monohydrate and elongated structures were present. Few unilamellar vesicles were occasionally visualized.CONCLUSIONS: The current study gives direct evidence, for the first time, of the existence of 'remnants' of lipolytic products in the fasted ascending colon. The impact of these structures to colonic absorption of drugs is an open question.
KW - Body Fluids
KW - Cholesterol
KW - Colitis, Ulcerative
KW - Colloids
KW - Colon, Ascending
KW - Cryoelectron Microscopy
KW - Gastrointestinal Contents
KW - Humans
KW - Microscopy, Electron, Transmission
KW - Physicochemical Phenomena
KW - Solubility
U2 - 10.1111/jphp.12128
DO - 10.1111/jphp.12128
M3 - Journal article
C2 - 24028615
VL - 65
SP - 1482
EP - 1487
JO - Journal of Pharmacy and Pharmacology
JF - Journal of Pharmacy and Pharmacology
SN - 0022-3573
IS - 10
ER -
ID: 120402736