UNITE: a database providing web-based methods for the molecular identification of ectomycorrhizal fungi

Research output: Contribution to journalJournal articleResearchpeer-review

  • U. Köljalg
  • K.H. Larsson
  • K. Abarenkov
  • H.R. Nilsson
  • I.J. Alexander
  • U. Eberhardt
  • S. Erland
  • Klaus Høiland
  • Kjøller, Rasmus
  • Ellen Larsson
  • Taina Pennanen
  • Robin Sen
  • Andy F. S. Taylor
  • Leho Tedersoo
  • Trude Vrålstad
  • Björn M. Ursing

Identification of ectomycorrhizal (ECM) fungi is often achieved through comparisons of ribosomal DNA internal transcribed spacer (ITS) sequences with accessioned sequences deposited in public databases. A major problem encountered is that annotation of the sequences in these databases is not always complete or trustworthy. In order to overcome this deficiency, we report on UNITE, an open-access database.

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    UNITE comprises well annotated fungal ITS sequences from well defined herbarium specimens that include full herbarium reference identification data, collector/source and ecological data. At present UNITE contains 758 ITS sequences from 455 species and 67 genera of ECM fungi.

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    UNITE can be searched by taxon name, via sequence similarity using blastn, and via phylogenetic sequence identification using galaxie. Following implementation, galaxie performs a phylogenetic analysis of the query sequence after alignment either to pre-existing generic alignments, or to matches retrieved from a blast search on the UNITE data. It should be noted that the current version of UNITE is dedicated to the reliable identification of ECM fungi.

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    The UNITE database is accessible through the URL http://unite.zbi.ee

Original languageEnglish
JournalNew Phytologist
Issue number3
Pages (from-to)1063-1068
Publication statusPublished - 2005

Bibliographical note

DNA taxonomy • ectomycorrhiza • fungi • molecular identification • sequence database • UNITE

ID: 87491