Ultrasmall TPGS-PLGA Hybrid Nanoparticles for Site-Specific Delivery of Antibiotics into Pseudomonas aeruginosa Biofilms in Lungs

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Ultrasmall TPGS-PLGA Hybrid Nanoparticles for Site-Specific Delivery of Antibiotics into Pseudomonas aeruginosa Biofilms in Lungs. / Wan, Feng; Bohr, Søren S.R.; Kłodzińska, Sylvia Natalie; Jumaa, Haidar; Huang, Zheng; Nylander, Tommy; Thygesen, Mikkel Boas; Sørensen, Kasper Kildegaard; Jensen, Knud Jørgen; Sternberg, Claus; Hatzakis, Nikos; Mørck Nielsen, Hanne.

In: ACS Applied Materials and Interfaces, Vol. 12, 2020, p. 380-389.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Wan, F, Bohr, SSR, Kłodzińska, SN, Jumaa, H, Huang, Z, Nylander, T, Thygesen, MB, Sørensen, KK, Jensen, KJ, Sternberg, C, Hatzakis, N & Mørck Nielsen, H 2020, 'Ultrasmall TPGS-PLGA Hybrid Nanoparticles for Site-Specific Delivery of Antibiotics into Pseudomonas aeruginosa Biofilms in Lungs', ACS Applied Materials and Interfaces, vol. 12, pp. 380-389. https://doi.org/10.1021/acsami.9b19644

APA

Wan, F., Bohr, S. S. R., Kłodzińska, S. N., Jumaa, H., Huang, Z., Nylander, T., ... Mørck Nielsen, H. (2020). Ultrasmall TPGS-PLGA Hybrid Nanoparticles for Site-Specific Delivery of Antibiotics into Pseudomonas aeruginosa Biofilms in Lungs. ACS Applied Materials and Interfaces, 12, 380-389. https://doi.org/10.1021/acsami.9b19644

Vancouver

Wan F, Bohr SSR, Kłodzińska SN, Jumaa H, Huang Z, Nylander T et al. Ultrasmall TPGS-PLGA Hybrid Nanoparticles for Site-Specific Delivery of Antibiotics into Pseudomonas aeruginosa Biofilms in Lungs. ACS Applied Materials and Interfaces. 2020;12:380-389. https://doi.org/10.1021/acsami.9b19644

Author

Wan, Feng ; Bohr, Søren S.R. ; Kłodzińska, Sylvia Natalie ; Jumaa, Haidar ; Huang, Zheng ; Nylander, Tommy ; Thygesen, Mikkel Boas ; Sørensen, Kasper Kildegaard ; Jensen, Knud Jørgen ; Sternberg, Claus ; Hatzakis, Nikos ; Mørck Nielsen, Hanne. / Ultrasmall TPGS-PLGA Hybrid Nanoparticles for Site-Specific Delivery of Antibiotics into Pseudomonas aeruginosa Biofilms in Lungs. In: ACS Applied Materials and Interfaces. 2020 ; Vol. 12. pp. 380-389.

Bibtex

@article{9acb9fd70cb44d479c2a0d78a1d08cef,
title = "Ultrasmall TPGS-PLGA Hybrid Nanoparticles for Site-Specific Delivery of Antibiotics into Pseudomonas aeruginosa Biofilms in Lungs",
abstract = "Inhaled antibiotic treatment of cystic fibrosis-related bacterial biofilm infections is challenging because of the pathological environment of the lungs. Here, we present an {"}environment-adaptive{"} nanoparticle composed of a solid poly lactic-co-glycolic acid (PLGA) core and a mucus-inert, enzymatically cleavable shell of d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) for the site-specific delivery of antibiotics to bacterial biofilms via aerosol administration. The hybrid nanoparticles with ultrasmall size were self-assembled via a nanoprecipitation process by using a facile microfluidic method. The interactions of the nanoparticles with the biological barriers were comprehensively investigated by using cutting-edge techniques (e.g., quartz crystal microbalance with dissipation monitoring, total internal reflection fluorescence microscopy-based particle tracking, in vitro biofilm model cultured in a flow-chamber system, and quantitative imaging analysis). Our results suggest that the mucus-inert, enzymatically cleavable TPGS shell enables the nanoparticles to penetrate through the mucus, accumulate in the deeper layer of the biofilms, and serve as sustained release depot, thereby improving the killing efficacy of azithromycin (a macrolide antibiotic) against biofilm-forming Pseudomonas aeruginosa. In conclusion, the ultrasmall TPGS-PLGA hybrid nanoparticles represent an efficient delivery system to overcome the multiple barriers and release antibiotics in a sustained manner in the vicinity of the biofilm-forming bacteria.",
keywords = "aerosol administration, bio-nano interaction, cystic fibrosis, Pseudomonas aeruginosa biofilm, TPGS-PLGA hybrid nanoparticles",
author = "Feng Wan and Bohr, {S{\o}ren S.R.} and Kłodzińska, {Sylvia Natalie} and Haidar Jumaa and Zheng Huang and Tommy Nylander and Thygesen, {Mikkel Boas} and S{\o}rensen, {Kasper Kildegaard} and Jensen, {Knud J{\o}rgen} and Claus Sternberg and Nikos Hatzakis and {M{\o}rck Nielsen}, Hanne",
year = "2020",
doi = "10.1021/acsami.9b19644",
language = "English",
volume = "12",
pages = "380--389",
journal = "A C S Applied Materials and Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",

}

RIS

TY - JOUR

T1 - Ultrasmall TPGS-PLGA Hybrid Nanoparticles for Site-Specific Delivery of Antibiotics into Pseudomonas aeruginosa Biofilms in Lungs

AU - Wan, Feng

AU - Bohr, Søren S.R.

AU - Kłodzińska, Sylvia Natalie

AU - Jumaa, Haidar

AU - Huang, Zheng

AU - Nylander, Tommy

AU - Thygesen, Mikkel Boas

AU - Sørensen, Kasper Kildegaard

AU - Jensen, Knud Jørgen

AU - Sternberg, Claus

AU - Hatzakis, Nikos

AU - Mørck Nielsen, Hanne

PY - 2020

Y1 - 2020

N2 - Inhaled antibiotic treatment of cystic fibrosis-related bacterial biofilm infections is challenging because of the pathological environment of the lungs. Here, we present an "environment-adaptive" nanoparticle composed of a solid poly lactic-co-glycolic acid (PLGA) core and a mucus-inert, enzymatically cleavable shell of d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) for the site-specific delivery of antibiotics to bacterial biofilms via aerosol administration. The hybrid nanoparticles with ultrasmall size were self-assembled via a nanoprecipitation process by using a facile microfluidic method. The interactions of the nanoparticles with the biological barriers were comprehensively investigated by using cutting-edge techniques (e.g., quartz crystal microbalance with dissipation monitoring, total internal reflection fluorescence microscopy-based particle tracking, in vitro biofilm model cultured in a flow-chamber system, and quantitative imaging analysis). Our results suggest that the mucus-inert, enzymatically cleavable TPGS shell enables the nanoparticles to penetrate through the mucus, accumulate in the deeper layer of the biofilms, and serve as sustained release depot, thereby improving the killing efficacy of azithromycin (a macrolide antibiotic) against biofilm-forming Pseudomonas aeruginosa. In conclusion, the ultrasmall TPGS-PLGA hybrid nanoparticles represent an efficient delivery system to overcome the multiple barriers and release antibiotics in a sustained manner in the vicinity of the biofilm-forming bacteria.

AB - Inhaled antibiotic treatment of cystic fibrosis-related bacterial biofilm infections is challenging because of the pathological environment of the lungs. Here, we present an "environment-adaptive" nanoparticle composed of a solid poly lactic-co-glycolic acid (PLGA) core and a mucus-inert, enzymatically cleavable shell of d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) for the site-specific delivery of antibiotics to bacterial biofilms via aerosol administration. The hybrid nanoparticles with ultrasmall size were self-assembled via a nanoprecipitation process by using a facile microfluidic method. The interactions of the nanoparticles with the biological barriers were comprehensively investigated by using cutting-edge techniques (e.g., quartz crystal microbalance with dissipation monitoring, total internal reflection fluorescence microscopy-based particle tracking, in vitro biofilm model cultured in a flow-chamber system, and quantitative imaging analysis). Our results suggest that the mucus-inert, enzymatically cleavable TPGS shell enables the nanoparticles to penetrate through the mucus, accumulate in the deeper layer of the biofilms, and serve as sustained release depot, thereby improving the killing efficacy of azithromycin (a macrolide antibiotic) against biofilm-forming Pseudomonas aeruginosa. In conclusion, the ultrasmall TPGS-PLGA hybrid nanoparticles represent an efficient delivery system to overcome the multiple barriers and release antibiotics in a sustained manner in the vicinity of the biofilm-forming bacteria.

KW - aerosol administration

KW - bio-nano interaction

KW - cystic fibrosis

KW - Pseudomonas aeruginosa biofilm

KW - TPGS-PLGA hybrid nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=85077111358&partnerID=8YFLogxK

U2 - 10.1021/acsami.9b19644

DO - 10.1021/acsami.9b19644

M3 - Journal article

C2 - 31804792

AN - SCOPUS:85077111358

VL - 12

SP - 380

EP - 389

JO - A C S Applied Materials and Interfaces

JF - A C S Applied Materials and Interfaces

SN - 1944-8244

ER -

ID: 235004620