Typing of vancomycin-resistant enterococci with MALDI-TOF mass spectrometry in a nosocomial outbreak setting

Research output: Contribution to journalJournal articlepeer-review

Standard

Typing of vancomycin-resistant enterococci with MALDI-TOF mass spectrometry in a nosocomial outbreak setting. / Holzknecht, B. J.; Dargis, R.; Pedersen, M.; Pinholt, M.; Christensen, J. J.; Hammerum, Anette M.; Littauer, Pia; Worning, Peder; Westh, Henrik; Moser, Claus; Justesen, Ulrik S.; Lemming, Lars E.; Søndergaard, Turid S.; Dzajic, Esad; Ejlertsen, Tove; the Danish Enterococcal Study Group.

In: Clinical Microbiology and Infection, Vol. 24, No. 10, 2018, p. 1104.e1-1104.e4.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Holzknecht, BJ, Dargis, R, Pedersen, M, Pinholt, M, Christensen, JJ, Hammerum, AM, Littauer, P, Worning, P, Westh, H, Moser, C, Justesen, US, Lemming, LE, Søndergaard, TS, Dzajic, E, Ejlertsen, T & the Danish Enterococcal Study Group 2018, 'Typing of vancomycin-resistant enterococci with MALDI-TOF mass spectrometry in a nosocomial outbreak setting', Clinical Microbiology and Infection, vol. 24, no. 10, pp. 1104.e1-1104.e4. https://doi.org/10.1016/j.cmi.2018.03.020

APA

Holzknecht, B. J., Dargis, R., Pedersen, M., Pinholt, M., Christensen, J. J., Hammerum, A. M., Littauer, P., Worning, P., Westh, H., Moser, C., Justesen, U. S., Lemming, L. E., Søndergaard, T. S., Dzajic, E., Ejlertsen, T., & the Danish Enterococcal Study Group (2018). Typing of vancomycin-resistant enterococci with MALDI-TOF mass spectrometry in a nosocomial outbreak setting. Clinical Microbiology and Infection, 24(10), 1104.e1-1104.e4. https://doi.org/10.1016/j.cmi.2018.03.020

Vancouver

Holzknecht BJ, Dargis R, Pedersen M, Pinholt M, Christensen JJ, Hammerum AM et al. Typing of vancomycin-resistant enterococci with MALDI-TOF mass spectrometry in a nosocomial outbreak setting. Clinical Microbiology and Infection. 2018;24(10):1104.e1-1104.e4. https://doi.org/10.1016/j.cmi.2018.03.020

Author

Holzknecht, B. J. ; Dargis, R. ; Pedersen, M. ; Pinholt, M. ; Christensen, J. J. ; Hammerum, Anette M. ; Littauer, Pia ; Worning, Peder ; Westh, Henrik ; Moser, Claus ; Justesen, Ulrik S. ; Lemming, Lars E. ; Søndergaard, Turid S. ; Dzajic, Esad ; Ejlertsen, Tove ; the Danish Enterococcal Study Group. / Typing of vancomycin-resistant enterococci with MALDI-TOF mass spectrometry in a nosocomial outbreak setting. In: Clinical Microbiology and Infection. 2018 ; Vol. 24, No. 10. pp. 1104.e1-1104.e4.

Bibtex

@article{3ee7460b886f4b468bf99aeef66359f0,
title = "Typing of vancomycin-resistant enterococci with MALDI-TOF mass spectrometry in a nosocomial outbreak setting",
abstract = "Objectives: To investigate the usefulness of matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS) typing as a first-line epidemiological tool in a nosocomial outbreak of vancomycin-resistant Enterococcus faecium (VREfm). Methods: Fifty-five VREfm isolates, previously characterized by whole-genome sequencing (WGS), were included and analysed by MALDI-TOF MS. To take peak reproducibility into account, ethanol/formic acid extraction and other steps of the protocol were conducted in triplicate. Twenty-seven spectra were generated per isolate, and spectra were visually inspected to determine discriminatory peaks. The presence or absence of these was recorded in a peak scheme. Results: Nine discriminatory peaks were identified. A characteristic pattern of these could distinguish between the three major WGS groups: WGS I, WGS II and WGS III. Only one of 38 isolates belonging to WGS I, WGS II or WGS III was misclassified. However, ten of the 17 isolates not belonging to WGS I, II or III displayed peak patterns indistinguishable from those of the outbreak strain. Conclusions: Using visual inspection of spectra, MALDI-TOF MS typing proved to be useful in differentiating three VREfm outbreak clones from each other. However, as non-outbreak isolates could not be reliably differentiated from outbreak clones, the practical value of this typing method for VREfm outbreak management was limited in our setting.",
keywords = "Enterococcus faecium, MALDI-TOF MS, Vancomycin resistance, VREfm",
author = "Holzknecht, {B. J.} and R. Dargis and M. Pedersen and M. Pinholt and Christensen, {J. J.} and Hammerum, {Anette M.} and Pia Littauer and Peder Worning and Henrik Westh and Claus Moser and Justesen, {Ulrik S.} and Lemming, {Lars E.} and S{\o}ndergaard, {Turid S.} and Esad Dzajic and Tove Ejlertsen and {the Danish Enterococcal Study Group}",
year = "2018",
doi = "10.1016/j.cmi.2018.03.020",
language = "English",
volume = "24",
pages = "1104.e1--1104.e4",
journal = "Clinical Microbiology and Infection",
issn = "1198-743X",
publisher = "Elsevier",
number = "10",

}

RIS

TY - JOUR

T1 - Typing of vancomycin-resistant enterococci with MALDI-TOF mass spectrometry in a nosocomial outbreak setting

AU - Holzknecht, B. J.

AU - Dargis, R.

AU - Pedersen, M.

AU - Pinholt, M.

AU - Christensen, J. J.

AU - Hammerum, Anette M.

AU - Littauer, Pia

AU - Worning, Peder

AU - Westh, Henrik

AU - Moser, Claus

AU - Justesen, Ulrik S.

AU - Lemming, Lars E.

AU - Søndergaard, Turid S.

AU - Dzajic, Esad

AU - Ejlertsen, Tove

AU - the Danish Enterococcal Study Group

PY - 2018

Y1 - 2018

N2 - Objectives: To investigate the usefulness of matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS) typing as a first-line epidemiological tool in a nosocomial outbreak of vancomycin-resistant Enterococcus faecium (VREfm). Methods: Fifty-five VREfm isolates, previously characterized by whole-genome sequencing (WGS), were included and analysed by MALDI-TOF MS. To take peak reproducibility into account, ethanol/formic acid extraction and other steps of the protocol were conducted in triplicate. Twenty-seven spectra were generated per isolate, and spectra were visually inspected to determine discriminatory peaks. The presence or absence of these was recorded in a peak scheme. Results: Nine discriminatory peaks were identified. A characteristic pattern of these could distinguish between the three major WGS groups: WGS I, WGS II and WGS III. Only one of 38 isolates belonging to WGS I, WGS II or WGS III was misclassified. However, ten of the 17 isolates not belonging to WGS I, II or III displayed peak patterns indistinguishable from those of the outbreak strain. Conclusions: Using visual inspection of spectra, MALDI-TOF MS typing proved to be useful in differentiating three VREfm outbreak clones from each other. However, as non-outbreak isolates could not be reliably differentiated from outbreak clones, the practical value of this typing method for VREfm outbreak management was limited in our setting.

AB - Objectives: To investigate the usefulness of matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS) typing as a first-line epidemiological tool in a nosocomial outbreak of vancomycin-resistant Enterococcus faecium (VREfm). Methods: Fifty-five VREfm isolates, previously characterized by whole-genome sequencing (WGS), were included and analysed by MALDI-TOF MS. To take peak reproducibility into account, ethanol/formic acid extraction and other steps of the protocol were conducted in triplicate. Twenty-seven spectra were generated per isolate, and spectra were visually inspected to determine discriminatory peaks. The presence or absence of these was recorded in a peak scheme. Results: Nine discriminatory peaks were identified. A characteristic pattern of these could distinguish between the three major WGS groups: WGS I, WGS II and WGS III. Only one of 38 isolates belonging to WGS I, WGS II or WGS III was misclassified. However, ten of the 17 isolates not belonging to WGS I, II or III displayed peak patterns indistinguishable from those of the outbreak strain. Conclusions: Using visual inspection of spectra, MALDI-TOF MS typing proved to be useful in differentiating three VREfm outbreak clones from each other. However, as non-outbreak isolates could not be reliably differentiated from outbreak clones, the practical value of this typing method for VREfm outbreak management was limited in our setting.

KW - Enterococcus faecium

KW - MALDI-TOF MS

KW - Vancomycin resistance

KW - VREfm

U2 - 10.1016/j.cmi.2018.03.020

DO - 10.1016/j.cmi.2018.03.020

M3 - Journal article

C2 - 29581054

AN - SCOPUS:85046151632

VL - 24

SP - 1104.e1-1104.e4

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

SN - 1198-743X

IS - 10

ER -

ID: 220844859