Two distinct pathways exist for down-regulation of the TCR

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Two distinct pathways exist for down-regulation of the TCR. / Lauritsen, J P; Christensen, M D; Dietrich, J; Kastrup, J; Odum, N; Geisler, C.

In: Journal of Immunology, Vol. 161, No. 1, 1998, p. 260-7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lauritsen, JP, Christensen, MD, Dietrich, J, Kastrup, J, Odum, N & Geisler, C 1998, 'Two distinct pathways exist for down-regulation of the TCR', Journal of Immunology, vol. 161, no. 1, pp. 260-7.

APA

Lauritsen, J. P., Christensen, M. D., Dietrich, J., Kastrup, J., Odum, N., & Geisler, C. (1998). Two distinct pathways exist for down-regulation of the TCR. Journal of Immunology, 161(1), 260-7.

Vancouver

Lauritsen JP, Christensen MD, Dietrich J, Kastrup J, Odum N, Geisler C. Two distinct pathways exist for down-regulation of the TCR. Journal of Immunology. 1998;161(1):260-7.

Author

Lauritsen, J P ; Christensen, M D ; Dietrich, J ; Kastrup, J ; Odum, N ; Geisler, C. / Two distinct pathways exist for down-regulation of the TCR. In: Journal of Immunology. 1998 ; Vol. 161, No. 1. pp. 260-7.

Bibtex

@article{2ae615f0b0a411ddb538000ea68e967b,

title = "Two distinct pathways exist for down-regulation of the TCR",

abstract = "TCR down-regulation plays an important role in modulating T cell responses both during T cell development and in mature T cells. Down-regulation of the TCR is induced by engagement of the TCR by specific ligands and/or by activation of protein kinase C (PKC). We report here that ligand- and PKC-induced TCR down-regulation is mediated by two distinct, independent mechanisms. Ligand-induced TCR down-regulation is dependent on the protein tyrosine kinases p56(lck) and p59(fyn) but independent of PKC and the CD3gamma leucine-based (L-based) internalization motif. In contrast, PKC-induced TCR down-regulation is dependent on the CD3gamma L-based internalization motif but independent of p56(lck) and p59(fyn). Finally, our data indicate that in the absence of TCR ligation, TCR expression levels can be finely regulated via the CD3gamma L-based motif by the balance between PKC and serine/threonine protein phosphatase activities. Such a TCR ligation-independent regulation of TCR expression levels could probably be important in determining the activation threshold of T cells in their encounter with APC.",

author = "Lauritsen, {J P} and Christensen, {M D} and J Dietrich and J Kastrup and N Odum and C Geisler",

note = "Keywords: Amino Acid Sequence; Down-Regulation; Humans; Jurkat Cells; Ligands; Molecular Sequence Data; Phosphoprotein Phosphatases; Phosphorylation; Protein Kinase C; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-fyn; Receptor-CD3 Complex, Antigen, T-Cell; Receptors, Antigen, T-Cell; T-Lymphocytes; Tyrosine; src-Family Kinases",

year = "1998",

language = "English",

volume = "161",

pages = "260--7",

journal = "Journal of Immunology",

issn = "0022-1767",

publisher = "American Association of Immunologists",

number = "1",

}

RIS

TY - JOUR

T1 - Two distinct pathways exist for down-regulation of the TCR

AU - Lauritsen, J P

AU - Christensen, M D

AU - Dietrich, J

AU - Kastrup, J

AU - Odum, N

AU - Geisler, C

N1 - Keywords: Amino Acid Sequence; Down-Regulation; Humans; Jurkat Cells; Ligands; Molecular Sequence Data; Phosphoprotein Phosphatases; Phosphorylation; Protein Kinase C; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-fyn; Receptor-CD3 Complex, Antigen, T-Cell; Receptors, Antigen, T-Cell; T-Lymphocytes; Tyrosine; src-Family Kinases

PY - 1998

Y1 - 1998

N2 - TCR down-regulation plays an important role in modulating T cell responses both during T cell development and in mature T cells. Down-regulation of the TCR is induced by engagement of the TCR by specific ligands and/or by activation of protein kinase C (PKC). We report here that ligand- and PKC-induced TCR down-regulation is mediated by two distinct, independent mechanisms. Ligand-induced TCR down-regulation is dependent on the protein tyrosine kinases p56(lck) and p59(fyn) but independent of PKC and the CD3gamma leucine-based (L-based) internalization motif. In contrast, PKC-induced TCR down-regulation is dependent on the CD3gamma L-based internalization motif but independent of p56(lck) and p59(fyn). Finally, our data indicate that in the absence of TCR ligation, TCR expression levels can be finely regulated via the CD3gamma L-based motif by the balance between PKC and serine/threonine protein phosphatase activities. Such a TCR ligation-independent regulation of TCR expression levels could probably be important in determining the activation threshold of T cells in their encounter with APC.

AB - TCR down-regulation plays an important role in modulating T cell responses both during T cell development and in mature T cells. Down-regulation of the TCR is induced by engagement of the TCR by specific ligands and/or by activation of protein kinase C (PKC). We report here that ligand- and PKC-induced TCR down-regulation is mediated by two distinct, independent mechanisms. Ligand-induced TCR down-regulation is dependent on the protein tyrosine kinases p56(lck) and p59(fyn) but independent of PKC and the CD3gamma leucine-based (L-based) internalization motif. In contrast, PKC-induced TCR down-regulation is dependent on the CD3gamma L-based internalization motif but independent of p56(lck) and p59(fyn). Finally, our data indicate that in the absence of TCR ligation, TCR expression levels can be finely regulated via the CD3gamma L-based motif by the balance between PKC and serine/threonine protein phosphatase activities. Such a TCR ligation-independent regulation of TCR expression levels could probably be important in determining the activation threshold of T cells in their encounter with APC.

M3 - Journal article

C2 - 9647232

VL - 161

SP - 260

EP - 267

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 1

ER -

ID: 8545462