Tryptophan depletion affects compulsive behaviour in rats: strain dependent effects and associated neuromechanisms
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Tryptophan depletion affects compulsive behaviour in rats : strain dependent effects and associated neuromechanisms. / Merchán, A; Navarro, S V; Klein, A B; Aznar, S; Campa, L; Suñol, C; Moreno, M; Flores, P.
In: Psychopharmacology, Vol. 234, No. 8, 04.2017, p. 1223-1236.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Tryptophan depletion affects compulsive behaviour in rats
T2 - strain dependent effects and associated neuromechanisms
AU - Merchán, A
AU - Navarro, S V
AU - Klein, A B
AU - Aznar, S
AU - Campa, L
AU - Suñol, C
AU - Moreno, M
AU - Flores, P
PY - 2017/4
Y1 - 2017/4
N2 - RATIONALE: Compulsive behaviour, present in different psychiatric disorders, such as obsessive-compulsive disorder, schizophrenia and drug abuse, is associated with altered levels of monoamines, particularly serotonin (5-hydroxytryptamine) and its receptor system.OBJECTIVES: The present study investigated whether 5-HT manipulation, through a tryptophan (TRP) depletion by diet in Wistar and Lister Hooded rats, modulates compulsive drinking in schedule-induced polydipsia (SIP) and locomotor activity in the open-field test. The levels of dopamine, noradrenaline, serotonin and its metabolite were evaluated, as well as the 5-HT2A and 5-HT1A receptor binding, in different brain regions.METHODS: Wistar rats were selected as high (HD) or low (LD) drinkers according to their SIP behaviour, while Lister hooded rats did not show SIP acquisition. Both strains were fed for 14 days with either a TRP-free diet (T-) or a TRP-supplemented diet (T+) RESULTS: The TRP depletion diet effectively reduced 5-HT levels in the frontal cortex, amygdala and hippocampus in both strains of rats. The TRP-depleted HD Wistar rats were more sensitive to 5-HT manipulation, exhibiting more licks on SIP than did the non-depleted HD Wistar rats, while the LD Wistar and the Lister Hooded rats did not exhibit differences in SIP. In contrast, the TRP-depleted Lister Hooded rats increased locomotor activity compared to the non-depleted rats, while no differences were found in the Wistar rats. Serotonin 2A receptor binding in the striatum was significantly reduced in the TRP-depleted HD Wistar rats.CONCLUSIONS: These results suggest that alterations of the serotonergic system could be involved in compulsive behaviour in vulnerable populations.
AB - RATIONALE: Compulsive behaviour, present in different psychiatric disorders, such as obsessive-compulsive disorder, schizophrenia and drug abuse, is associated with altered levels of monoamines, particularly serotonin (5-hydroxytryptamine) and its receptor system.OBJECTIVES: The present study investigated whether 5-HT manipulation, through a tryptophan (TRP) depletion by diet in Wistar and Lister Hooded rats, modulates compulsive drinking in schedule-induced polydipsia (SIP) and locomotor activity in the open-field test. The levels of dopamine, noradrenaline, serotonin and its metabolite were evaluated, as well as the 5-HT2A and 5-HT1A receptor binding, in different brain regions.METHODS: Wistar rats were selected as high (HD) or low (LD) drinkers according to their SIP behaviour, while Lister hooded rats did not show SIP acquisition. Both strains were fed for 14 days with either a TRP-free diet (T-) or a TRP-supplemented diet (T+) RESULTS: The TRP depletion diet effectively reduced 5-HT levels in the frontal cortex, amygdala and hippocampus in both strains of rats. The TRP-depleted HD Wistar rats were more sensitive to 5-HT manipulation, exhibiting more licks on SIP than did the non-depleted HD Wistar rats, while the LD Wistar and the Lister Hooded rats did not exhibit differences in SIP. In contrast, the TRP-depleted Lister Hooded rats increased locomotor activity compared to the non-depleted rats, while no differences were found in the Wistar rats. Serotonin 2A receptor binding in the striatum was significantly reduced in the TRP-depleted HD Wistar rats.CONCLUSIONS: These results suggest that alterations of the serotonergic system could be involved in compulsive behaviour in vulnerable populations.
KW - Amygdala
KW - Analysis of Variance
KW - Animals
KW - Behavior, Animal
KW - Brain
KW - Compulsive Behavior
KW - Diet
KW - Disease Models, Animal
KW - Dopamine
KW - Drinking
KW - Hippocampus
KW - Male
KW - Neostriatum
KW - Polydipsia
KW - Rats
KW - Rats, Wistar
KW - Receptor, Serotonin, 5-HT1A
KW - Receptor, Serotonin, 5-HT2A
KW - Serotonin
KW - Tryptophan
KW - Journal Article
U2 - 10.1007/s00213-017-4561-5
DO - 10.1007/s00213-017-4561-5
M3 - Journal article
C2 - 28280881
VL - 234
SP - 1223
EP - 1236
JO - Psychopharmacology
JF - Psychopharmacology
SN - 0033-3158
IS - 8
ER -
ID: 187579969