Trefoil factor peptides in serum and sputum from subjects with asthma and COPD
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Trefoil factor peptides in serum and sputum from subjects with asthma and COPD. / Viby, Niels-Erik; Pedersen, Lars; Lund, Thomas Kromann; Kissow, Hannelouise; Backer, Vibeke; Nexø, Ebba; Thim, Lars; Poulsen, Steen Seier.
In: Clinical Respiratory Journal, Vol. 9, No. 3, 07.2015, p. 322-329.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Trefoil factor peptides in serum and sputum from subjects with asthma and COPD
AU - Viby, Niels-Erik
AU - Pedersen, Lars
AU - Lund, Thomas Kromann
AU - Kissow, Hannelouise
AU - Backer, Vibeke
AU - Nexø, Ebba
AU - Thim, Lars
AU - Poulsen, Steen Seier
N1 - This article is protected by copyright. All rights reserved.
PY - 2015/7
Y1 - 2015/7
N2 - Objective: Trefoil factor peptides (TFF) are secreted onto mucosal surfaces together with mucins and occur in high concentrations in pulmonary secretions from patients with chronic obstructive pulmonary disease (COPD). In the present study, we aimed to explore the concentrations of the peptides in serum and sputum in patients with COPD.Materials and Methods: Thirty-five individuals were included in the study, including 11 healthy individuals, 13 indivials with asthma and 11 individuals with COPD. TFF1, TFF2 and TFF3 were measured by enzyme-linked immunosorbent assay (ELISA) in sputum induced by hypertonic saline inhalation and in serum. Total protein content in sputum was also determined.Results: In the sputum samples from COPD patients, we observed an eightfold higher concentration of TFF1 and a fivefold higher concentration of TFF3 compared with controls. In the serum samples from COPD patients, we observed three-, three- and twofold higher concentrations of TFF1, TFF2 and TFF3 respectively compared with controls.Conclusions: There is increased secretion of TFF peptides in the lungs of patients with COPD, as well as significant increases in serum levels. This suggests a role for TFF peptides in the pathogenesis of pulmonary diseases with mucus hypersecretion.
AB - Objective: Trefoil factor peptides (TFF) are secreted onto mucosal surfaces together with mucins and occur in high concentrations in pulmonary secretions from patients with chronic obstructive pulmonary disease (COPD). In the present study, we aimed to explore the concentrations of the peptides in serum and sputum in patients with COPD.Materials and Methods: Thirty-five individuals were included in the study, including 11 healthy individuals, 13 indivials with asthma and 11 individuals with COPD. TFF1, TFF2 and TFF3 were measured by enzyme-linked immunosorbent assay (ELISA) in sputum induced by hypertonic saline inhalation and in serum. Total protein content in sputum was also determined.Results: In the sputum samples from COPD patients, we observed an eightfold higher concentration of TFF1 and a fivefold higher concentration of TFF3 compared with controls. In the serum samples from COPD patients, we observed three-, three- and twofold higher concentrations of TFF1, TFF2 and TFF3 respectively compared with controls.Conclusions: There is increased secretion of TFF peptides in the lungs of patients with COPD, as well as significant increases in serum levels. This suggests a role for TFF peptides in the pathogenesis of pulmonary diseases with mucus hypersecretion.
U2 - 10.1111/crj.12146
DO - 10.1111/crj.12146
M3 - Journal article
C2 - 24720774
VL - 9
SP - 322
EP - 329
JO - Clinical Respiratory Journal
JF - Clinical Respiratory Journal
SN - 1752-6981
IS - 3
ER -
ID: 162895069