Trapped ion mobility spectrometry and PASEF enable in-depth lipidomics from minimal sample amounts

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Trapped ion mobility spectrometry and PASEF enable in-depth lipidomics from minimal sample amounts. / Vasilopoulou, Catherine G; Sulek, Karolina; Brunner, Andreas-David; Meitei, Ningombam Sanjib; Schweiger-Hufnagel, Ulrike; Meyer, Sven W; Barsch, Aiko; Mann, Matthias; Meier, Florian.

In: Nature Communications, Vol. 11, No. 1, 331, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Vasilopoulou, CG, Sulek, K, Brunner, A-D, Meitei, NS, Schweiger-Hufnagel, U, Meyer, SW, Barsch, A, Mann, M & Meier, F 2020, 'Trapped ion mobility spectrometry and PASEF enable in-depth lipidomics from minimal sample amounts', Nature Communications, vol. 11, no. 1, 331. https://doi.org/10.1038/s41467-019-14044-x

APA

Vasilopoulou, C. G., Sulek, K., Brunner, A-D., Meitei, N. S., Schweiger-Hufnagel, U., Meyer, S. W., Barsch, A., Mann, M., & Meier, F. (2020). Trapped ion mobility spectrometry and PASEF enable in-depth lipidomics from minimal sample amounts. Nature Communications, 11(1), [331]. https://doi.org/10.1038/s41467-019-14044-x

Vancouver

Vasilopoulou CG, Sulek K, Brunner A-D, Meitei NS, Schweiger-Hufnagel U, Meyer SW et al. Trapped ion mobility spectrometry and PASEF enable in-depth lipidomics from minimal sample amounts. Nature Communications. 2020;11(1). 331. https://doi.org/10.1038/s41467-019-14044-x

Author

Vasilopoulou, Catherine G ; Sulek, Karolina ; Brunner, Andreas-David ; Meitei, Ningombam Sanjib ; Schweiger-Hufnagel, Ulrike ; Meyer, Sven W ; Barsch, Aiko ; Mann, Matthias ; Meier, Florian. / Trapped ion mobility spectrometry and PASEF enable in-depth lipidomics from minimal sample amounts. In: Nature Communications. 2020 ; Vol. 11, No. 1.

Bibtex

@article{0afe2aa434eb4ecea76831280a6cfe4b,
title = "Trapped ion mobility spectrometry and PASEF enable in-depth lipidomics from minimal sample amounts",
abstract = "A comprehensive characterization of the lipidome from limited starting material remains very challenging. Here we report a high-sensitivity lipidomics workflow based on nanoflow liquid chromatography and trapped ion mobility spectrometry (TIMS). Taking advantage of parallel accumulation-serial fragmentation (PASEF), we fragment on average 15 precursors in each of 100 ms TIMS scans, while maintaining the full mobility resolution of co-eluting isomers. The acquisition speed of over 100 Hz allows us to obtain MS/MS spectra of the vast majority of isotope patterns. Analyzing 1 µL of human plasma, PASEF increases the number of identified lipids more than three times over standard TIMS-MS/MS, achieving attomole sensitivity. Building on high intra- and inter-laboratory precision and accuracy of TIMS collisional cross sections (CCS), we compile 1856 lipid CCS values from plasma, liver and cancer cells. Our study establishes PASEF in lipid analysis and paves the way for sensitive, ion mobility-enhanced lipidomics in four dimensions.",
author = "Vasilopoulou, {Catherine G} and Karolina Sulek and Andreas-David Brunner and Meitei, {Ningombam Sanjib} and Ulrike Schweiger-Hufnagel and Meyer, {Sven W} and Aiko Barsch and Matthias Mann and Florian Meier",
year = "2020",
doi = "10.1038/s41467-019-14044-x",
language = "English",
volume = "11",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Trapped ion mobility spectrometry and PASEF enable in-depth lipidomics from minimal sample amounts

AU - Vasilopoulou, Catherine G

AU - Sulek, Karolina

AU - Brunner, Andreas-David

AU - Meitei, Ningombam Sanjib

AU - Schweiger-Hufnagel, Ulrike

AU - Meyer, Sven W

AU - Barsch, Aiko

AU - Mann, Matthias

AU - Meier, Florian

PY - 2020

Y1 - 2020

N2 - A comprehensive characterization of the lipidome from limited starting material remains very challenging. Here we report a high-sensitivity lipidomics workflow based on nanoflow liquid chromatography and trapped ion mobility spectrometry (TIMS). Taking advantage of parallel accumulation-serial fragmentation (PASEF), we fragment on average 15 precursors in each of 100 ms TIMS scans, while maintaining the full mobility resolution of co-eluting isomers. The acquisition speed of over 100 Hz allows us to obtain MS/MS spectra of the vast majority of isotope patterns. Analyzing 1 µL of human plasma, PASEF increases the number of identified lipids more than three times over standard TIMS-MS/MS, achieving attomole sensitivity. Building on high intra- and inter-laboratory precision and accuracy of TIMS collisional cross sections (CCS), we compile 1856 lipid CCS values from plasma, liver and cancer cells. Our study establishes PASEF in lipid analysis and paves the way for sensitive, ion mobility-enhanced lipidomics in four dimensions.

AB - A comprehensive characterization of the lipidome from limited starting material remains very challenging. Here we report a high-sensitivity lipidomics workflow based on nanoflow liquid chromatography and trapped ion mobility spectrometry (TIMS). Taking advantage of parallel accumulation-serial fragmentation (PASEF), we fragment on average 15 precursors in each of 100 ms TIMS scans, while maintaining the full mobility resolution of co-eluting isomers. The acquisition speed of over 100 Hz allows us to obtain MS/MS spectra of the vast majority of isotope patterns. Analyzing 1 µL of human plasma, PASEF increases the number of identified lipids more than three times over standard TIMS-MS/MS, achieving attomole sensitivity. Building on high intra- and inter-laboratory precision and accuracy of TIMS collisional cross sections (CCS), we compile 1856 lipid CCS values from plasma, liver and cancer cells. Our study establishes PASEF in lipid analysis and paves the way for sensitive, ion mobility-enhanced lipidomics in four dimensions.

U2 - 10.1038/s41467-019-14044-x

DO - 10.1038/s41467-019-14044-x

M3 - Journal article

C2 - 31949144

VL - 11

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 331

ER -

ID: 239207391