The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition

Research output: Contribution to journalJournal articlepeer-review

Documents

  • Fulltext

    Final published version, 7.04 MB, PDF document

It has been proposed that ATR kinase senses the completion of DNA replication to initiate the S/G2 transition. In contrast to this model, we show here that the TRESLIN-MTBP complex prevents a premature entry into G2 from early S-phase independently of ATR/CHK1 kinases. TRESLIN-MTBP acts transiently at pre-replication complexes (preRCs) to initiate origin firing and is released after the subsequent recruitment of CDC45. This dynamic behavior of TRESLIN-MTBP implements a monitoring system that checks the activation of replication forks and senses the rate of origin firing to prevent the entry into G2. This system detects the decline in the number of origins of replication that naturally occurs in very late S, which is the signature that cells use to determine the completion of DNA replication and permit the S/G2 transition. Our work introduces TRESLIN-MTBP as a key player in cell-cycle control independent of canonical checkpoints.

Original languageEnglish
JournalMolecular Cell
Volume82
Issue number18
Pages (from-to)3350-3365.e7
Number of pages24
ISSN1097-2765
DOIs
Publication statusPublished - 2022

Bibliographical note

Publisher Copyright:
© 2022 The Authors

    Research areas

  • ATR, cell cycle, checkpoint, CHK1, DNA replication, MTBP, origin firing, S/G2 transition, TRESLIN

Number of downloads are based on statistics from Google Scholar and www.ku.dk


No data available

ID: 321473826