The transcriptome of hand eczema assessed by tape stripping

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The transcriptome of hand eczema assessed by tape stripping. / Sølberg, Julie B.K.; Quaade, Anna S.; Jacobsen, Stine B.; Andersen, Jeppe D.; Kampmann, Marie Louise; Morling, Niels; Litman, Thomas; Thyssen, Jacob P.; Johansen, Jeanne D.

In: Contact Dermatitis, Vol. 86, No. 2, 2022, p. 71-79.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sølberg, JBK, Quaade, AS, Jacobsen, SB, Andersen, JD, Kampmann, ML, Morling, N, Litman, T, Thyssen, JP & Johansen, JD 2022, 'The transcriptome of hand eczema assessed by tape stripping', Contact Dermatitis, vol. 86, no. 2, pp. 71-79. https://doi.org/10.1111/cod.14015

APA

Sølberg, J. B. K., Quaade, A. S., Jacobsen, S. B., Andersen, J. D., Kampmann, M. L., Morling, N., Litman, T., Thyssen, J. P., & Johansen, J. D. (2022). The transcriptome of hand eczema assessed by tape stripping. Contact Dermatitis, 86(2), 71-79. https://doi.org/10.1111/cod.14015

Vancouver

Sølberg JBK, Quaade AS, Jacobsen SB, Andersen JD, Kampmann ML, Morling N et al. The transcriptome of hand eczema assessed by tape stripping. Contact Dermatitis. 2022;86(2):71-79. https://doi.org/10.1111/cod.14015

Author

Sølberg, Julie B.K. ; Quaade, Anna S. ; Jacobsen, Stine B. ; Andersen, Jeppe D. ; Kampmann, Marie Louise ; Morling, Niels ; Litman, Thomas ; Thyssen, Jacob P. ; Johansen, Jeanne D. / The transcriptome of hand eczema assessed by tape stripping. In: Contact Dermatitis. 2022 ; Vol. 86, No. 2. pp. 71-79.

Bibtex

@article{c7d8c19b057d435081585ec3e3e9bb7c,

title = "The transcriptome of hand eczema assessed by tape stripping",

abstract = "Background: No biomarkers have been identified that can classify subtypes of hand eczema (HE). Although skin biopsies represent the gold standard for investigations of the skin, the invasive technique is not favorable when investigating skin from sensitive areas. Recent advances in the use of skin-tape strips for molecular investigations enable noninvasive investigations of HE. Objective: By using whole transcriptome sequencing (WTS), the molecular profile of HE according to different localizations on the hands, etiologies, and clinical/morphological subtypes was investigated. Methods: Thirty adult, Danish HE patients, 12 with and 18 without concurrent atopic dermatitis (AD), as well as 16 controls were included. Tape strip samples were collected from lesional, nonlesional, and healthy skin. Total RNA was extracted and WTS was performed. Results: The largest molecular difference of HE patients with and without AD was found in nonlesional skin areas and included a downregulation of CXCL8 for HE patients without AD. Differences between allergic and irritant contact dermatitis included promising epidermal biomarkers such as EPHA1. Conclusion: Skin tape strip samples could be used to assess the gene expression profile of HE on different localizations of the hands. The skin tape strip method identified new molecular markers that showed promising result for the identification of HE subtypes.",

keywords = "atopic hand eczema, contact dermatitis, subtypes of hand eczema, tape stripping, transcriptomics",

author = "S{\o}lberg, {Julie B.K.} and Quaade, {Anna S.} and Jacobsen, {Stine B.} and Andersen, {Jeppe D.} and Kampmann, {Marie Louise} and Niels Morling and Thomas Litman and Thyssen, {Jacob P.} and Johansen, {Jeanne D.}",

note = "Publisher Copyright: {\textcopyright} 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd",

year = "2022",

doi = "10.1111/cod.14015",

language = "English",

volume = "86",

pages = "71--79",

journal = "Contact Dermatitis",

issn = "0105-1873",

publisher = "Wiley-Blackwell",

number = "2",

}

RIS

TY - JOUR

T1 - The transcriptome of hand eczema assessed by tape stripping

AU - Sølberg, Julie B.K.

AU - Quaade, Anna S.

AU - Jacobsen, Stine B.

AU - Andersen, Jeppe D.

AU - Kampmann, Marie Louise

AU - Morling, Niels

AU - Litman, Thomas

AU - Thyssen, Jacob P.

AU - Johansen, Jeanne D.

PY - 2022

Y1 - 2022

N2 - Background: No biomarkers have been identified that can classify subtypes of hand eczema (HE). Although skin biopsies represent the gold standard for investigations of the skin, the invasive technique is not favorable when investigating skin from sensitive areas. Recent advances in the use of skin-tape strips for molecular investigations enable noninvasive investigations of HE. Objective: By using whole transcriptome sequencing (WTS), the molecular profile of HE according to different localizations on the hands, etiologies, and clinical/morphological subtypes was investigated. Methods: Thirty adult, Danish HE patients, 12 with and 18 without concurrent atopic dermatitis (AD), as well as 16 controls were included. Tape strip samples were collected from lesional, nonlesional, and healthy skin. Total RNA was extracted and WTS was performed. Results: The largest molecular difference of HE patients with and without AD was found in nonlesional skin areas and included a downregulation of CXCL8 for HE patients without AD. Differences between allergic and irritant contact dermatitis included promising epidermal biomarkers such as EPHA1. Conclusion: Skin tape strip samples could be used to assess the gene expression profile of HE on different localizations of the hands. The skin tape strip method identified new molecular markers that showed promising result for the identification of HE subtypes.

AB - Background: No biomarkers have been identified that can classify subtypes of hand eczema (HE). Although skin biopsies represent the gold standard for investigations of the skin, the invasive technique is not favorable when investigating skin from sensitive areas. Recent advances in the use of skin-tape strips for molecular investigations enable noninvasive investigations of HE. Objective: By using whole transcriptome sequencing (WTS), the molecular profile of HE according to different localizations on the hands, etiologies, and clinical/morphological subtypes was investigated. Methods: Thirty adult, Danish HE patients, 12 with and 18 without concurrent atopic dermatitis (AD), as well as 16 controls were included. Tape strip samples were collected from lesional, nonlesional, and healthy skin. Total RNA was extracted and WTS was performed. Results: The largest molecular difference of HE patients with and without AD was found in nonlesional skin areas and included a downregulation of CXCL8 for HE patients without AD. Differences between allergic and irritant contact dermatitis included promising epidermal biomarkers such as EPHA1. Conclusion: Skin tape strip samples could be used to assess the gene expression profile of HE on different localizations of the hands. The skin tape strip method identified new molecular markers that showed promising result for the identification of HE subtypes.

KW - atopic hand eczema

KW - contact dermatitis

KW - subtypes of hand eczema

KW - tape stripping

KW - transcriptomics

U2 - 10.1111/cod.14015

DO - 10.1111/cod.14015

M3 - Journal article

C2 - 34812515

AN - SCOPUS:85120791754

VL - 86

SP - 71

EP - 79

JO - Contact Dermatitis

JF - Contact Dermatitis

SN - 0105-1873

IS - 2

ER -

ID: 287705142