The prevalence of controls in phyllosphere microbiome research: a methodological review

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DNA contamination can critically confound microbiome studies. Here, we take a systematic approach to review the current literature and investigate the prevalence of contamination controls in phyllosphere microbiome research over the past decade. By utilising systematic review principles for this review, we were able to conduct a thorough investigation, screening 450 articles from three databases for eligibility and extracting data in a controlled and methodical manner. Worryingly, we observed a surprisingly low usage of both positive and negative contamination controls in phyllosphere research. As a result, we propose a set of minimum standards to combat the effects of contamination in future phyllosphere research.

Original languageEnglish
JournalNew Phytologist
Issue number1
Pages (from-to)23-29
Number of pages7
Publication statusPublished - 2024

Bibliographical note

Publisher Copyright:
© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.

    Research areas

  • contamination, controls, epiphytic, microbiome, microbiota, phyllosphere, plants, standards

ID: 383733910