Aging is associated with changes of the prokaryotic component of the gut microbiota - becoming less diverse, and increasing proinflammatory profile compared to younger adults. These changes have been linked with frailty in several studies. However, our knowledge of the influence of the gut mycobiome on health and disease in elderly remain sparsely investigated. Consequently, the aim of this study was to characterise the feacal mycobiota in relation to host health parameters.Feacal samples from 99 healthy individuals ranging from 65 to 81 years old were collected, and fungal composition was determined using ITS2 amplicon sequencing. The obtained sequences were analysed non-parametrically using the QIIME pipeline to assess fungal taxa composition and diversity based on alpha and beta diversity, respectively. ANOSIM and adonis analyses were performed to assess the significance level between categories associated with the clinical features among individuals.The elderly gut is home to three main phyla Ascomycota, Basidiomycota and Zygomycota, with genera Penicillium, Candida, and Aspergillus being particularly common. Based on HbA1c-levels, the individuals could be clustered into 3 groups, High, Medium and Healthy. Clusters according to genus abundance co-segregated with glycated glucose levels (HbA1c), carbohydrate intake, and insulin secretion based on C-peptide levels. Interestingly, the dissimilarity matrices of Bray-Curtis showed significant (P<0.05) variation between clusters with glycated glucose level.Collectively, these findings suggest that the presences of specific gut mycobiome member is associated with glycemic behaviours among the healthy individuals of the elderly Danes population.