The GAGOme: a cell-based library of displayed glycosaminoglycans

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The GAGOme : a cell-based library of displayed glycosaminoglycans. / Chen, Yen-Hsi; Narimatsu, Yoshiki; Clausen, Thomas Mandel; Gomes, Catarina; Karlsson, Richard; Steentoft, Catharina; Spliid, Charlotte Bredo; Gustavsson, Tobias; Salanti, Ali; Persson, Andrea; Malmström, Anders; Willén, Daniel; Ellervik, Ulf; Bennett, Eric Paul; Mao, Yang; Clausen, Henrik; Yang, Zhang.

In: Nature Methods, Vol. 15, No. 11, 2018, p. 881-888.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Chen, Y-H, Narimatsu, Y, Clausen, TM, Gomes, C, Karlsson, R, Steentoft, C, Spliid, CB, Gustavsson, T, Salanti, A, Persson, A, Malmström, A, Willén, D, Ellervik, U, Bennett, EP, Mao, Y, Clausen, H & Yang, Z 2018, 'The GAGOme: a cell-based library of displayed glycosaminoglycans', Nature Methods, vol. 15, no. 11, pp. 881-888. https://doi.org/10.1038/s41592-018-0086-z

APA

Chen, Y-H., Narimatsu, Y., Clausen, T. M., Gomes, C., Karlsson, R., Steentoft, C., Spliid, C. B., Gustavsson, T., Salanti, A., Persson, A., Malmström, A., Willén, D., Ellervik, U., Bennett, E. P., Mao, Y., Clausen, H., & Yang, Z. (2018). The GAGOme: a cell-based library of displayed glycosaminoglycans. Nature Methods, 15(11), 881-888. https://doi.org/10.1038/s41592-018-0086-z

Vancouver

Chen Y-H, Narimatsu Y, Clausen TM, Gomes C, Karlsson R, Steentoft C et al. The GAGOme: a cell-based library of displayed glycosaminoglycans. Nature Methods. 2018;15(11):881-888. https://doi.org/10.1038/s41592-018-0086-z

Author

Chen, Yen-Hsi ; Narimatsu, Yoshiki ; Clausen, Thomas Mandel ; Gomes, Catarina ; Karlsson, Richard ; Steentoft, Catharina ; Spliid, Charlotte Bredo ; Gustavsson, Tobias ; Salanti, Ali ; Persson, Andrea ; Malmström, Anders ; Willén, Daniel ; Ellervik, Ulf ; Bennett, Eric Paul ; Mao, Yang ; Clausen, Henrik ; Yang, Zhang. / The GAGOme : a cell-based library of displayed glycosaminoglycans. In: Nature Methods. 2018 ; Vol. 15, No. 11. pp. 881-888.

Bibtex

@article{1a40647ce39c440fad98062eed962229,
title = "The GAGOme: a cell-based library of displayed glycosaminoglycans",
abstract = "Glycosaminoglycans (GAGs) are essential polysaccharides in normal physiology and disease. However, understanding of the contribution of specific GAG structures to specific biological functions is limited, largely because of the great structural heterogeneity among GAGs themselves, as well as technical limitations in the structural characterization and chemical synthesis of GAGs. Here we describe a cell-based method to produce and display distinct GAGs with a broad repertoire of modifications, a library we refer to as the GAGOme. By using precise gene editing, we engineered a large panel of Chinese hamster ovary cells with knockout or knock-in of the genes encoding most of the enzymes involved in GAG biosynthesis, to generate a library of isogenic cell lines that differentially display distinct GAG features. We show that this library can be used for cell-based binding assays, recombinant expression of proteoglycans with distinct GAG structures, and production of distinct GAG chains on metabolic primers that may be used for the assembly of GAG glycan microarrays.",
author = "Yen-Hsi Chen and Yoshiki Narimatsu and Clausen, {Thomas Mandel} and Catarina Gomes and Richard Karlsson and Catharina Steentoft and Spliid, {Charlotte Bredo} and Tobias Gustavsson and Ali Salanti and Andrea Persson and Anders Malmstr{\"o}m and Daniel Will{\'e}n and Ulf Ellervik and Bennett, {Eric Paul} and Yang Mao and Henrik Clausen and Zhang Yang",
year = "2018",
doi = "10.1038/s41592-018-0086-z",
language = "English",
volume = "15",
pages = "881--888",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "nature publishing group",
number = "11",

}

RIS

TY - JOUR

T1 - The GAGOme

T2 - a cell-based library of displayed glycosaminoglycans

AU - Chen, Yen-Hsi

AU - Narimatsu, Yoshiki

AU - Clausen, Thomas Mandel

AU - Gomes, Catarina

AU - Karlsson, Richard

AU - Steentoft, Catharina

AU - Spliid, Charlotte Bredo

AU - Gustavsson, Tobias

AU - Salanti, Ali

AU - Persson, Andrea

AU - Malmström, Anders

AU - Willén, Daniel

AU - Ellervik, Ulf

AU - Bennett, Eric Paul

AU - Mao, Yang

AU - Clausen, Henrik

AU - Yang, Zhang

PY - 2018

Y1 - 2018

N2 - Glycosaminoglycans (GAGs) are essential polysaccharides in normal physiology and disease. However, understanding of the contribution of specific GAG structures to specific biological functions is limited, largely because of the great structural heterogeneity among GAGs themselves, as well as technical limitations in the structural characterization and chemical synthesis of GAGs. Here we describe a cell-based method to produce and display distinct GAGs with a broad repertoire of modifications, a library we refer to as the GAGOme. By using precise gene editing, we engineered a large panel of Chinese hamster ovary cells with knockout or knock-in of the genes encoding most of the enzymes involved in GAG biosynthesis, to generate a library of isogenic cell lines that differentially display distinct GAG features. We show that this library can be used for cell-based binding assays, recombinant expression of proteoglycans with distinct GAG structures, and production of distinct GAG chains on metabolic primers that may be used for the assembly of GAG glycan microarrays.

AB - Glycosaminoglycans (GAGs) are essential polysaccharides in normal physiology and disease. However, understanding of the contribution of specific GAG structures to specific biological functions is limited, largely because of the great structural heterogeneity among GAGs themselves, as well as technical limitations in the structural characterization and chemical synthesis of GAGs. Here we describe a cell-based method to produce and display distinct GAGs with a broad repertoire of modifications, a library we refer to as the GAGOme. By using precise gene editing, we engineered a large panel of Chinese hamster ovary cells with knockout or knock-in of the genes encoding most of the enzymes involved in GAG biosynthesis, to generate a library of isogenic cell lines that differentially display distinct GAG features. We show that this library can be used for cell-based binding assays, recombinant expression of proteoglycans with distinct GAG structures, and production of distinct GAG chains on metabolic primers that may be used for the assembly of GAG glycan microarrays.

U2 - 10.1038/s41592-018-0086-z

DO - 10.1038/s41592-018-0086-z

M3 - Journal article

C2 - 30104636

VL - 15

SP - 881

EP - 888

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 11

ER -

ID: 201046743