The effect of whole genome amplification on samples originating from more than one donor
Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review
In this study, the GenomiPhi(TM) DNA Amplification Kit (Amersham Biosciences) was used to investigate the potential of whole genome amplification (WGA) when considering samples originating from more than one donor. DNA was extracted from blood samples, quantified and normalised before being mixed in ratios of 1:1, 1:3, 1:7 and 1:15. Proportions were checked using standard STR analysis before being subjected to WGA. Once amplified using GenomiPhi(TM), the ratios were again checked. Relative proportions were found to be maintained in the 1:1 and 1:3 ratios following WGA; the observed peak ratios were found to match the expected peak ratios regardless of the starting concentration of DNA. With samples mixed in the ratio of 1:7 and 1:15, and when the concentration of starting material was at the manufacturer's lower limit, too few minor component peaks were found to allow for statistical analysis. With an initial template exceeding 1 ng/[mu]L there was an increase in problems associated with profile interpretation but the results obtained indicated that mixture proportions could be quantifiably maintained
Original language | English |
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Title of host publication | Progress in Forensic Genetics 11 : Proceedings of the 21st International ISFG Congress |
Number of pages | 2 |
Volume | 1288 |
Publisher | Elsevier |
Publication date | 2006 |
Pages | 722-724 |
DOIs | |
Publication status | Published - 2006 |
Event | Progress in Forensic Genetics 11 - Proceedings of the 21st International ISFG Congress - Ponta Delgada, The Azores, Portugal Duration: 13 Sep 2005 → 16 Sep 2005 |
Conference
Conference | Progress in Forensic Genetics 11 - Proceedings of the 21st International ISFG Congress |
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Land | Portugal |
By | Ponta Delgada, The Azores |
Periode | 13/09/2005 → 16/09/2005 |
Series | ICS - International Congress Series |
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ID: 18251470