The effect of pH and ionic strength on the fluorescence properties of a red emissive DNA-stabilized silver nanocluster
Research output: Contribution to journal › Journal article › Research › peer-review
DNA-stabilized silver nanoclusters (DNA-AgNCs) are a class of promising fluorophores for imaging and sensing applications. All aspects of their spectroscopic properties are not yet fully characterized, leaving this field still with a number of fundamental studies to be addressed. In this work, we studied the spectroscopic properties of red-emitting DNA-AgNCs at different pH (5 to 9) and ionic strength μ (0.005 to 0.5). The photophysical properties of high performance liquid chromatography (HPLC) purified DNA-AgNCs proved to be constant over a large range of pH and μ, with absorption, emission and fluorescence decay times only being affected at very high pH and μ values. Non-purified DNA-AgNCs were also unaffected by pH and/or μ variations, but significant differences can be observed between the rotational correlation times of purified and non-purified DNA-AgNCs.
Original language | English |
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Journal | Methods and Applications in Fluorescence |
Volume | 8 |
Issue number | 1 |
Pages (from-to) | 014005 |
Number of pages | 8 |
ISSN | 2050-6120 |
DOIs | |
Publication status | Published - 3 Dec 2019 |
- Biosensing Techniques, Chromatography, High Pressure Liquid, DNA/chemistry, Fluorescence, Hydrogen-Ion Concentration, Metal Nanoparticles/chemistry, Osmolar Concentration, Silver/chemistry, Spectrometry, Fluorescence, Time Factors
Research areas
ID: 243151498