The effect of increasing membrane curvature on the phase transition and mixing behavior of a dimyristoyl-sn-glycero-3-phosphatidylcholine/ distearoyl-sn-glycero-3-phosphatidylcholine lipid mixture as studied by fourier transform infrared spectroscopy and differential scanning calorimetry

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The effect of increasing membrane curvature on the phase transition and mixing behavior of a dimyristoyl-sn-glycero-3-phosphatidylcholine/ distearoyl-sn-glycero-3-phosphatidylcholine lipid mixture as studied by fourier transform infrared spectroscopy and differential scanning calorimetry. / Brumm, Thomas; Jørgensen, Kent; Mouritsen, Ole G.; Bayerl, Thomas M.

In: Biophysical Journal, Vol. 70, No. 3, 03.1996, p. 1373-1379.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Brumm, T, Jørgensen, K, Mouritsen, OG & Bayerl, TM 1996, 'The effect of increasing membrane curvature on the phase transition and mixing behavior of a dimyristoyl-sn-glycero-3-phosphatidylcholine/ distearoyl-sn-glycero-3-phosphatidylcholine lipid mixture as studied by fourier transform infrared spectroscopy and differential scanning calorimetry', Biophysical Journal, vol. 70, no. 3, pp. 1373-1379. https://doi.org/10.1016/S0006-3495(96)79695-9

APA

Brumm, T., Jørgensen, K., Mouritsen, O. G., & Bayerl, T. M. (1996). The effect of increasing membrane curvature on the phase transition and mixing behavior of a dimyristoyl-sn-glycero-3-phosphatidylcholine/ distearoyl-sn-glycero-3-phosphatidylcholine lipid mixture as studied by fourier transform infrared spectroscopy and differential scanning calorimetry. Biophysical Journal, 70(3), 1373-1379. https://doi.org/10.1016/S0006-3495(96)79695-9

Vancouver

Brumm T, Jørgensen K, Mouritsen OG, Bayerl TM. The effect of increasing membrane curvature on the phase transition and mixing behavior of a dimyristoyl-sn-glycero-3-phosphatidylcholine/ distearoyl-sn-glycero-3-phosphatidylcholine lipid mixture as studied by fourier transform infrared spectroscopy and differential scanning calorimetry. Biophysical Journal. 1996 Mar;70(3):1373-1379. https://doi.org/10.1016/S0006-3495(96)79695-9

Author

Brumm, Thomas ; Jørgensen, Kent ; Mouritsen, Ole G. ; Bayerl, Thomas M. / The effect of increasing membrane curvature on the phase transition and mixing behavior of a dimyristoyl-sn-glycero-3-phosphatidylcholine/ distearoyl-sn-glycero-3-phosphatidylcholine lipid mixture as studied by fourier transform infrared spectroscopy and differential scanning calorimetry. In: Biophysical Journal. 1996 ; Vol. 70, No. 3. pp. 1373-1379.

Bibtex

@article{05c75ac4c68d453aa422db215e3c6b9a,
title = "The effect of increasing membrane curvature on the phase transition and mixing behavior of a dimyristoyl-sn-glycero-3-phosphatidylcholine/ distearoyl-sn-glycero-3-phosphatidylcholine lipid mixture as studied by fourier transform infrared spectroscopy and differential scanning calorimetry",
abstract = "The phase transition behavior of a lipid bilayer of dimyristoyl-sn-glycero-3-phosphatidylcholine/distearoyl-snglycero-3- phosphatidylcholine (DMPC-d54/DSPC) (1:1) on a solid support with varying curvatures was investigated with differential scanning calorimetry, infrared spectroscopy, and model calculations. With increasing curvature the temperatures of the liquidus and solidus points are shifted to lower values by up to 7°C and 15°C, and the mixing of the two lipid species in the two phase region is altered. With increasing curvature the DSPC dominates the gel phase, whereas the DMPC-d54 is expelled to the fluid phase. Whereas the planar system shows a nearly simultaneous phase transition of DSPC and DMPC-d54, the spherical system with the highest curvature exhibits an almost complete separation of the phase transitions of the two lipids. Model calculations suggest that the shift of the liquidus point can be understood as a reduction of the lateral pressure in the bilayer with increasing curvature. The shift of the solidus line is interpreted as a result of the increased demixing of the two components in the two-phase region with increasing curvature due to lowering of the lateral pressure.",
author = "Thomas Brumm and Kent J{\o}rgensen and Mouritsen, {Ole G.} and Bayerl, {Thomas M.}",
year = "1996",
month = mar,
doi = "10.1016/S0006-3495(96)79695-9",
language = "English",
volume = "70",
pages = "1373--1379",
journal = "Biophysical Society. Annual Meeting. Abstracts",
issn = "0523-6800",
publisher = "Biophysical Society",
number = "3",

}

RIS

TY - JOUR

T1 - The effect of increasing membrane curvature on the phase transition and mixing behavior of a dimyristoyl-sn-glycero-3-phosphatidylcholine/ distearoyl-sn-glycero-3-phosphatidylcholine lipid mixture as studied by fourier transform infrared spectroscopy and differential scanning calorimetry

AU - Brumm, Thomas

AU - Jørgensen, Kent

AU - Mouritsen, Ole G.

AU - Bayerl, Thomas M.

PY - 1996/3

Y1 - 1996/3

N2 - The phase transition behavior of a lipid bilayer of dimyristoyl-sn-glycero-3-phosphatidylcholine/distearoyl-snglycero-3- phosphatidylcholine (DMPC-d54/DSPC) (1:1) on a solid support with varying curvatures was investigated with differential scanning calorimetry, infrared spectroscopy, and model calculations. With increasing curvature the temperatures of the liquidus and solidus points are shifted to lower values by up to 7°C and 15°C, and the mixing of the two lipid species in the two phase region is altered. With increasing curvature the DSPC dominates the gel phase, whereas the DMPC-d54 is expelled to the fluid phase. Whereas the planar system shows a nearly simultaneous phase transition of DSPC and DMPC-d54, the spherical system with the highest curvature exhibits an almost complete separation of the phase transitions of the two lipids. Model calculations suggest that the shift of the liquidus point can be understood as a reduction of the lateral pressure in the bilayer with increasing curvature. The shift of the solidus line is interpreted as a result of the increased demixing of the two components in the two-phase region with increasing curvature due to lowering of the lateral pressure.

AB - The phase transition behavior of a lipid bilayer of dimyristoyl-sn-glycero-3-phosphatidylcholine/distearoyl-snglycero-3- phosphatidylcholine (DMPC-d54/DSPC) (1:1) on a solid support with varying curvatures was investigated with differential scanning calorimetry, infrared spectroscopy, and model calculations. With increasing curvature the temperatures of the liquidus and solidus points are shifted to lower values by up to 7°C and 15°C, and the mixing of the two lipid species in the two phase region is altered. With increasing curvature the DSPC dominates the gel phase, whereas the DMPC-d54 is expelled to the fluid phase. Whereas the planar system shows a nearly simultaneous phase transition of DSPC and DMPC-d54, the spherical system with the highest curvature exhibits an almost complete separation of the phase transitions of the two lipids. Model calculations suggest that the shift of the liquidus point can be understood as a reduction of the lateral pressure in the bilayer with increasing curvature. The shift of the solidus line is interpreted as a result of the increased demixing of the two components in the two-phase region with increasing curvature due to lowering of the lateral pressure.

UR - http://www.scopus.com/inward/record.url?scp=0030053808&partnerID=8YFLogxK

U2 - 10.1016/S0006-3495(96)79695-9

DO - 10.1016/S0006-3495(96)79695-9

M3 - Journal article

C2 - 8785292

AN - SCOPUS:0030053808

VL - 70

SP - 1373

EP - 1379

JO - Biophysical Society. Annual Meeting. Abstracts

JF - Biophysical Society. Annual Meeting. Abstracts

SN - 0523-6800

IS - 3

ER -

ID: 236887999