The effect of gender on early colonic anastomotic wound healing

Research output: Contribution to journalJournal articlepeer-review

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The effect of gender on early colonic anastomotic wound healing. / Kjaer, Marie; Kristjánsdóttir, Hrefna; Andersen, Line; Heegaard, Anne Marie; Ågren, Magnus S.; Jorgensen, Lars N.

In: International Journal of Colorectal Disease, Vol. 33, No. 9, 2018, p. 1269-1276.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Kjaer, M, Kristjánsdóttir, H, Andersen, L, Heegaard, AM, Ågren, MS & Jorgensen, LN 2018, 'The effect of gender on early colonic anastomotic wound healing', International Journal of Colorectal Disease, vol. 33, no. 9, pp. 1269-1276. https://doi.org/10.1007/s00384-018-3089-4

APA

Kjaer, M., Kristjánsdóttir, H., Andersen, L., Heegaard, A. M., Ågren, M. S., & Jorgensen, L. N. (2018). The effect of gender on early colonic anastomotic wound healing. International Journal of Colorectal Disease, 33(9), 1269-1276. https://doi.org/10.1007/s00384-018-3089-4

Vancouver

Kjaer M, Kristjánsdóttir H, Andersen L, Heegaard AM, Ågren MS, Jorgensen LN. The effect of gender on early colonic anastomotic wound healing. International Journal of Colorectal Disease. 2018;33(9):1269-1276. https://doi.org/10.1007/s00384-018-3089-4

Author

Kjaer, Marie ; Kristjánsdóttir, Hrefna ; Andersen, Line ; Heegaard, Anne Marie ; Ågren, Magnus S. ; Jorgensen, Lars N. / The effect of gender on early colonic anastomotic wound healing. In: International Journal of Colorectal Disease. 2018 ; Vol. 33, No. 9. pp. 1269-1276.

Bibtex

@article{2bd08cf7dff447b5bbf9395b45d4a7d5,
title = "The effect of gender on early colonic anastomotic wound healing",
abstract = "Purpose: Clinically, male patients subjected to colorectal surgery are more prone to develop anastomotic leakage than female patients by unknown mechanisms. Our aim was to investigate the impact of gender on anastomotic wound healing using an experimental model. Methods: One-layer colonic anastomosis was constructed in 8-week-old 28 male and 32 female Sprague-Dawley rats. Animals of one group (n = 30) were sacrificed immediately after surgery day 0 and the other group (n = 30) on postoperative day 3. Anastomotic breaking strength, total collagen (hydroxyproline), soluble collagen (Sircol), matrix metalloproteinase (MMP)-9, and transforming growth factor (TGF)-β1 were measured. Results: The anastomotic breaking strength decreased from day 0 to day 3 with no significant gender differences either in the extent of decline (P = 0.122) or absolute day 3 strengths (P = 0.425). Analogously, total collagen concentration in the anastomotic wounds decreased postoperatively and were lower (P = 0.043) in the male compared with the female rats on day 3. MMP-9 levels increased in the anastomoses postoperatively, but they did not differ (P = 0.391) between male and female animals. Soluble collagen levels were lower in the day-3 anastomoses of male versus female rats (P = 0.015) and correlated positively with total TGF-β1 levels (rS = 0.540, P = 0.006). Although TGF-β1 tended to be lower in male compared with the female rats, the differences did not reach statistical significance. Conclusion: Our findings point towards a less favorable collagen metabolism in colonic anastomoses of male compared with female rats during early wound healing.",
keywords = "Anastomotic leakage, Androgens, Breaking strength, Collagen, Colorectal surgery, Gender differences",
author = "Marie Kjaer and Hrefna Kristj{\'a}nsd{\'o}ttir and Line Andersen and Heegaard, {Anne Marie} and {\AA}gren, {Magnus S.} and Jorgensen, {Lars N.}",
year = "2018",
doi = "10.1007/s00384-018-3089-4",
language = "English",
volume = "33",
pages = "1269--1276",
journal = "International Journal of Colorectal Disease",
issn = "0179-1958",
publisher = "Springer",
number = "9",

}

RIS

TY - JOUR

T1 - The effect of gender on early colonic anastomotic wound healing

AU - Kjaer, Marie

AU - Kristjánsdóttir, Hrefna

AU - Andersen, Line

AU - Heegaard, Anne Marie

AU - Ågren, Magnus S.

AU - Jorgensen, Lars N.

PY - 2018

Y1 - 2018

N2 - Purpose: Clinically, male patients subjected to colorectal surgery are more prone to develop anastomotic leakage than female patients by unknown mechanisms. Our aim was to investigate the impact of gender on anastomotic wound healing using an experimental model. Methods: One-layer colonic anastomosis was constructed in 8-week-old 28 male and 32 female Sprague-Dawley rats. Animals of one group (n = 30) were sacrificed immediately after surgery day 0 and the other group (n = 30) on postoperative day 3. Anastomotic breaking strength, total collagen (hydroxyproline), soluble collagen (Sircol), matrix metalloproteinase (MMP)-9, and transforming growth factor (TGF)-β1 were measured. Results: The anastomotic breaking strength decreased from day 0 to day 3 with no significant gender differences either in the extent of decline (P = 0.122) or absolute day 3 strengths (P = 0.425). Analogously, total collagen concentration in the anastomotic wounds decreased postoperatively and were lower (P = 0.043) in the male compared with the female rats on day 3. MMP-9 levels increased in the anastomoses postoperatively, but they did not differ (P = 0.391) between male and female animals. Soluble collagen levels were lower in the day-3 anastomoses of male versus female rats (P = 0.015) and correlated positively with total TGF-β1 levels (rS = 0.540, P = 0.006). Although TGF-β1 tended to be lower in male compared with the female rats, the differences did not reach statistical significance. Conclusion: Our findings point towards a less favorable collagen metabolism in colonic anastomoses of male compared with female rats during early wound healing.

AB - Purpose: Clinically, male patients subjected to colorectal surgery are more prone to develop anastomotic leakage than female patients by unknown mechanisms. Our aim was to investigate the impact of gender on anastomotic wound healing using an experimental model. Methods: One-layer colonic anastomosis was constructed in 8-week-old 28 male and 32 female Sprague-Dawley rats. Animals of one group (n = 30) were sacrificed immediately after surgery day 0 and the other group (n = 30) on postoperative day 3. Anastomotic breaking strength, total collagen (hydroxyproline), soluble collagen (Sircol), matrix metalloproteinase (MMP)-9, and transforming growth factor (TGF)-β1 were measured. Results: The anastomotic breaking strength decreased from day 0 to day 3 with no significant gender differences either in the extent of decline (P = 0.122) or absolute day 3 strengths (P = 0.425). Analogously, total collagen concentration in the anastomotic wounds decreased postoperatively and were lower (P = 0.043) in the male compared with the female rats on day 3. MMP-9 levels increased in the anastomoses postoperatively, but they did not differ (P = 0.391) between male and female animals. Soluble collagen levels were lower in the day-3 anastomoses of male versus female rats (P = 0.015) and correlated positively with total TGF-β1 levels (rS = 0.540, P = 0.006). Although TGF-β1 tended to be lower in male compared with the female rats, the differences did not reach statistical significance. Conclusion: Our findings point towards a less favorable collagen metabolism in colonic anastomoses of male compared with female rats during early wound healing.

KW - Anastomotic leakage

KW - Androgens

KW - Breaking strength

KW - Collagen

KW - Colorectal surgery

KW - Gender differences

U2 - 10.1007/s00384-018-3089-4

DO - 10.1007/s00384-018-3089-4

M3 - Journal article

C2 - 29850942

AN - SCOPUS:85047796196

VL - 33

SP - 1269

EP - 1276

JO - International Journal of Colorectal Disease

JF - International Journal of Colorectal Disease

SN - 0179-1958

IS - 9

ER -

ID: 220859178