The discovery of bacterial biofilm in patients with muscle invasive bladder cancer
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The discovery of bacterial biofilm in patients with muscle invasive bladder cancer. / Nadler, Naomi; Kvich, Lasse; Bjarnsholt, Thomas; Jensen, Jorgen Bjerggaard; Gogenur, Ismail; Azawi, Nessn.
In: APMIS, Vol. 129, No. 5, 2021, p. 265-270.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - The discovery of bacterial biofilm in patients with muscle invasive bladder cancer
AU - Nadler, Naomi
AU - Kvich, Lasse
AU - Bjarnsholt, Thomas
AU - Jensen, Jorgen Bjerggaard
AU - Gogenur, Ismail
AU - Azawi, Nessn
PY - 2021
Y1 - 2021
N2 - The carcinogenic effects of microorganisms have been discovered in multiple cancer types. In urology, the development of squamous cell carcinoma of the bladder due to the parasitic infection with Schistosoma Mansoni is widely accepted. The oncogenic potential of biofilms has been studied in colorectal cancer and experimental studies have shown that bacteria such as Escherichia coli drive the development of colorectal cancer. Notably, Escherichia coli is responsible for 80% of all urinary tract infections. Recent findings suggest an altered urinary microbiome in patients with bladder cancer compared to healthy subjects. In this case series, we demonstrate our findings of biofilm formation in human bladder cancer tissue. Tissue samples from ten patients that underwent routine Transurethral Resection of Bladder Tumor (TURBT) were obtained from the Danish National Biobank. Pathological tissue was examined for presence of bacterial aggregates by Fluorescence in situ Hybridization. In two of ten patients, analysis showed abundant bacterial aggregation on the surface epithelium. Both positive cases had pT2 urothelial bladder cancer. Our findings suggest that biofilm occurs in urothelial cancer tissue indicating an association between biofilm formation and bladder cancer.
AB - The carcinogenic effects of microorganisms have been discovered in multiple cancer types. In urology, the development of squamous cell carcinoma of the bladder due to the parasitic infection with Schistosoma Mansoni is widely accepted. The oncogenic potential of biofilms has been studied in colorectal cancer and experimental studies have shown that bacteria such as Escherichia coli drive the development of colorectal cancer. Notably, Escherichia coli is responsible for 80% of all urinary tract infections. Recent findings suggest an altered urinary microbiome in patients with bladder cancer compared to healthy subjects. In this case series, we demonstrate our findings of biofilm formation in human bladder cancer tissue. Tissue samples from ten patients that underwent routine Transurethral Resection of Bladder Tumor (TURBT) were obtained from the Danish National Biobank. Pathological tissue was examined for presence of bacterial aggregates by Fluorescence in situ Hybridization. In two of ten patients, analysis showed abundant bacterial aggregation on the surface epithelium. Both positive cases had pT2 urothelial bladder cancer. Our findings suggest that biofilm occurs in urothelial cancer tissue indicating an association between biofilm formation and bladder cancer.
KW - Bladder cancer
KW - microbiome
KW - translational science
U2 - 10.1111/apm.13097
DO - 10.1111/apm.13097
M3 - Journal article
C2 - 33205550
VL - 129
SP - 265
EP - 270
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
IS - 5
ER -
ID: 259623412