The Arabidopsis PLAT domain protein1 is critically involved in abiotic stress tolerance

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

The Arabidopsis PLAT domain protein1 is critically involved in abiotic stress tolerance. / Hyun, Tae Kyung; van der Graaff, Eric; Albacete, Alfonso; Eom, Seung Hee; Grosskinsky, Dominik Kilian; Böhm, Hannah; Janschek, Ursula; Rim, Yeonggil; Ali, Walid Wahid; Kim, Soo Young; Roitsch, Thomas Georg.

In: PLOS ONE, Vol. 9, e112946, 2014.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Hyun, TK, van der Graaff, E, Albacete, A, Eom, SH, Grosskinsky, DK, Böhm, H, Janschek, U, Rim, Y, Ali, WW, Kim, SY & Roitsch, TG 2014, 'The Arabidopsis PLAT domain protein1 is critically involved in abiotic stress tolerance', PLOS ONE, vol. 9, e112946. https://doi.org/10.1371/journal.pone.0112946

APA

Hyun, T. K., van der Graaff, E., Albacete, A., Eom, S. H., Grosskinsky, D. K., Böhm, H., ... Roitsch, T. G. (2014). The Arabidopsis PLAT domain protein1 is critically involved in abiotic stress tolerance. PLOS ONE, 9, [e112946]. https://doi.org/10.1371/journal.pone.0112946

Vancouver

Hyun TK, van der Graaff E, Albacete A, Eom SH, Grosskinsky DK, Böhm H et al. The Arabidopsis PLAT domain protein1 is critically involved in abiotic stress tolerance. PLOS ONE. 2014;9. e112946. https://doi.org/10.1371/journal.pone.0112946

Author

Hyun, Tae Kyung ; van der Graaff, Eric ; Albacete, Alfonso ; Eom, Seung Hee ; Grosskinsky, Dominik Kilian ; Böhm, Hannah ; Janschek, Ursula ; Rim, Yeonggil ; Ali, Walid Wahid ; Kim, Soo Young ; Roitsch, Thomas Georg. / The Arabidopsis PLAT domain protein1 is critically involved in abiotic stress tolerance. In: PLOS ONE. 2014 ; Vol. 9.

Bibtex

@article{2d899124ff9c43de819f6ca6d529bf20,
title = "The Arabidopsis PLAT domain protein1 is critically involved in abiotic stress tolerance",
abstract = "Despite the completion of the Arabidopsis genome sequence, for only a relatively low percentage of the encoded proteins experimental evidence concerning their function is available. Plant proteins that harbour a single PLAT (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipase) domain and belong to the PLAT-plant-stress protein family are ubiquitously present in monocot and dicots. However, the function of PLAT-plant-stress proteins is still poorly understood. Therefore, we have assessed the function of the uncharacterised Arabidopsis PLAT-plant-stress family members through a combination of functional genetic and physiological approaches. PLAT1 overexpression conferred increased abiotic stress tolerance, including cold, drought and salt stress, while loss-of-function resulted in opposite effects on abiotic stress tolerance. Strikingly, PLAT1 promoted growth under non-stressed conditions. Abiotic stress treatments induced PLAT1 expression and caused expansion of its expression domain. The ABF/ABRE transcription factors, which are positive mediators of abscisic acid signalling, activate PLAT1 promoter activity in transactivation assays and directly bind to the ABRE elements located in this promoter in electrophoretic mobility shift assays. This suggests that PLAT1 represents a novel downstream target of the abscisic acid signalling pathway. Thus, we showed that PLAT1 critically functions as positive regulator of abiotic stress tolerance, but also is involved in regulating plant growth, and thereby assigned a function to this previously uncharacterised PLAT domain protein. The functional data obtained for PLAT1 support that PLAT-plant-stress proteins in general could be promising targets for improving abiotic stress tolerance without yield penalty.",
author = "Hyun, {Tae Kyung} and {van der Graaff}, Eric and Alfonso Albacete and Eom, {Seung Hee} and Grosskinsky, {Dominik Kilian} and Hannah B{\"o}hm and Ursula Janschek and Yeonggil Rim and Ali, {Walid Wahid} and Kim, {Soo Young} and Roitsch, {Thomas Georg}",
note = "OA",
year = "2014",
doi = "10.1371/journal.pone.0112946",
language = "English",
volume = "9",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",

}

RIS

TY - JOUR

T1 - The Arabidopsis PLAT domain protein1 is critically involved in abiotic stress tolerance

AU - Hyun, Tae Kyung

AU - van der Graaff, Eric

AU - Albacete, Alfonso

AU - Eom, Seung Hee

AU - Grosskinsky, Dominik Kilian

AU - Böhm, Hannah

AU - Janschek, Ursula

AU - Rim, Yeonggil

AU - Ali, Walid Wahid

AU - Kim, Soo Young

AU - Roitsch, Thomas Georg

N1 - OA

PY - 2014

Y1 - 2014

N2 - Despite the completion of the Arabidopsis genome sequence, for only a relatively low percentage of the encoded proteins experimental evidence concerning their function is available. Plant proteins that harbour a single PLAT (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipase) domain and belong to the PLAT-plant-stress protein family are ubiquitously present in monocot and dicots. However, the function of PLAT-plant-stress proteins is still poorly understood. Therefore, we have assessed the function of the uncharacterised Arabidopsis PLAT-plant-stress family members through a combination of functional genetic and physiological approaches. PLAT1 overexpression conferred increased abiotic stress tolerance, including cold, drought and salt stress, while loss-of-function resulted in opposite effects on abiotic stress tolerance. Strikingly, PLAT1 promoted growth under non-stressed conditions. Abiotic stress treatments induced PLAT1 expression and caused expansion of its expression domain. The ABF/ABRE transcription factors, which are positive mediators of abscisic acid signalling, activate PLAT1 promoter activity in transactivation assays and directly bind to the ABRE elements located in this promoter in electrophoretic mobility shift assays. This suggests that PLAT1 represents a novel downstream target of the abscisic acid signalling pathway. Thus, we showed that PLAT1 critically functions as positive regulator of abiotic stress tolerance, but also is involved in regulating plant growth, and thereby assigned a function to this previously uncharacterised PLAT domain protein. The functional data obtained for PLAT1 support that PLAT-plant-stress proteins in general could be promising targets for improving abiotic stress tolerance without yield penalty.

AB - Despite the completion of the Arabidopsis genome sequence, for only a relatively low percentage of the encoded proteins experimental evidence concerning their function is available. Plant proteins that harbour a single PLAT (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipase) domain and belong to the PLAT-plant-stress protein family are ubiquitously present in monocot and dicots. However, the function of PLAT-plant-stress proteins is still poorly understood. Therefore, we have assessed the function of the uncharacterised Arabidopsis PLAT-plant-stress family members through a combination of functional genetic and physiological approaches. PLAT1 overexpression conferred increased abiotic stress tolerance, including cold, drought and salt stress, while loss-of-function resulted in opposite effects on abiotic stress tolerance. Strikingly, PLAT1 promoted growth under non-stressed conditions. Abiotic stress treatments induced PLAT1 expression and caused expansion of its expression domain. The ABF/ABRE transcription factors, which are positive mediators of abscisic acid signalling, activate PLAT1 promoter activity in transactivation assays and directly bind to the ABRE elements located in this promoter in electrophoretic mobility shift assays. This suggests that PLAT1 represents a novel downstream target of the abscisic acid signalling pathway. Thus, we showed that PLAT1 critically functions as positive regulator of abiotic stress tolerance, but also is involved in regulating plant growth, and thereby assigned a function to this previously uncharacterised PLAT domain protein. The functional data obtained for PLAT1 support that PLAT-plant-stress proteins in general could be promising targets for improving abiotic stress tolerance without yield penalty.

U2 - 10.1371/journal.pone.0112946

DO - 10.1371/journal.pone.0112946

M3 - Journal article

C2 - 25396746

AN - SCOPUS:84911935198

VL - 9

JO - P L o S One

JF - P L o S One

SN - 1932-6203

M1 - e112946

ER -

ID: 129923570