Systems-wide analysis of BCR signalosomes and downstream phosphorylation and ubiquitylation
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Systems-wide analysis of BCR signalosomes and downstream phosphorylation and ubiquitylation. / Satpathy, Shankha; Wagner, Sebastian A; Beli, Petra; Gupta, Rajat; Kristiansen, Trine A; Malinova, Dessislava; Francavilla, Chiara; Tolar, Pavel; Bishop, Gail A; Hostager, Bruce S; Choudhary, Chuna Ram.
In: Molecular Systems Biology, Vol. 11, No. 6, 810, 2015.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Systems-wide analysis of BCR signalosomes and downstream phosphorylation and ubiquitylation
AU - Satpathy, Shankha
AU - Wagner, Sebastian A
AU - Beli, Petra
AU - Gupta, Rajat
AU - Kristiansen, Trine A
AU - Malinova, Dessislava
AU - Francavilla, Chiara
AU - Tolar, Pavel
AU - Bishop, Gail A
AU - Hostager, Bruce S
AU - Choudhary, Chuna Ram
N1 - © 2015 The Authors. Published under the terms of the CC BY 4.0 license.
PY - 2015
Y1 - 2015
N2 - B-cell receptor (BCR) signaling is essential for the development and function of B cells; however, the spectrum of proteins involved in BCR signaling is not fully known. Here we used quantitative mass spectrometry-based proteomics to monitor the dynamics of BCR signaling complexes (signalosomes) and to investigate the dynamics of downstream phosphorylation and ubiquitylation signaling. We identify most of the previously known components of BCR signaling, as well as many proteins that have not yet been implicated in this system. BCR activation leads to rapid tyrosine phosphorylation and ubiquitylation of the receptor-proximal signaling components, many of which are co-regulated by both the modifications. We illustrate the power of multilayered proteomic analyses for discovering novel BCR signaling components by demonstrating that BCR-induced phosphorylation of RAB7A at S72 prevents its association with effector proteins and with endo-lysosomal compartments. In addition, we show that BCL10 is modified by LUBAC-mediated linear ubiquitylation, and demonstrate an important function of LUBAC in BCR-induced NF-κB signaling. Our results offer a global and integrated view of BCR signaling, and the provided datasets can serve as a valuable resource for further understanding BCR signaling networks.
AB - B-cell receptor (BCR) signaling is essential for the development and function of B cells; however, the spectrum of proteins involved in BCR signaling is not fully known. Here we used quantitative mass spectrometry-based proteomics to monitor the dynamics of BCR signaling complexes (signalosomes) and to investigate the dynamics of downstream phosphorylation and ubiquitylation signaling. We identify most of the previously known components of BCR signaling, as well as many proteins that have not yet been implicated in this system. BCR activation leads to rapid tyrosine phosphorylation and ubiquitylation of the receptor-proximal signaling components, many of which are co-regulated by both the modifications. We illustrate the power of multilayered proteomic analyses for discovering novel BCR signaling components by demonstrating that BCR-induced phosphorylation of RAB7A at S72 prevents its association with effector proteins and with endo-lysosomal compartments. In addition, we show that BCL10 is modified by LUBAC-mediated linear ubiquitylation, and demonstrate an important function of LUBAC in BCR-induced NF-κB signaling. Our results offer a global and integrated view of BCR signaling, and the provided datasets can serve as a valuable resource for further understanding BCR signaling networks.
U2 - 10.15252/msb.20145880
DO - 10.15252/msb.20145880
M3 - Journal article
C2 - 26038114
VL - 11
JO - Molecular Systems Biology
JF - Molecular Systems Biology
SN - 1744-4292
IS - 6
M1 - 810
ER -
ID: 140068809