Super-resolution microscopy reveals functional organization of dopamine transporters into cholesterol and neuronal activity-dependent nanodomains

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Super-resolution microscopy reveals functional organization of dopamine transporters into cholesterol and neuronal activity-dependent nanodomains. / Rahbek-Clemmensen, Troels; Lycas, Matthew D.; Erlendsson, Simon; Eriksen, Jacob; Apuschkin, Mia; Vilhardt, Frederik; Jorgensen, Trine N.; Hansen, Freja H.; Gether, Ulrik.

In: Nature Communications, Vol. 8, 740, 2017.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Rahbek-Clemmensen, T, Lycas, MD, Erlendsson, S, Eriksen, J, Apuschkin, M, Vilhardt, F, Jorgensen, TN, Hansen, FH & Gether, U 2017, 'Super-resolution microscopy reveals functional organization of dopamine transporters into cholesterol and neuronal activity-dependent nanodomains', Nature Communications, vol. 8, 740. https://doi.org/10.1038/s41467-017-00790-3

APA

Rahbek-Clemmensen, T., Lycas, M. D., Erlendsson, S., Eriksen, J., Apuschkin, M., Vilhardt, F., ... Gether, U. (2017). Super-resolution microscopy reveals functional organization of dopamine transporters into cholesterol and neuronal activity-dependent nanodomains. Nature Communications, 8, [740]. https://doi.org/10.1038/s41467-017-00790-3

Vancouver

Rahbek-Clemmensen T, Lycas MD, Erlendsson S, Eriksen J, Apuschkin M, Vilhardt F et al. Super-resolution microscopy reveals functional organization of dopamine transporters into cholesterol and neuronal activity-dependent nanodomains. Nature Communications. 2017;8. 740. https://doi.org/10.1038/s41467-017-00790-3

Author

Rahbek-Clemmensen, Troels ; Lycas, Matthew D. ; Erlendsson, Simon ; Eriksen, Jacob ; Apuschkin, Mia ; Vilhardt, Frederik ; Jorgensen, Trine N. ; Hansen, Freja H. ; Gether, Ulrik. / Super-resolution microscopy reveals functional organization of dopamine transporters into cholesterol and neuronal activity-dependent nanodomains. In: Nature Communications. 2017 ; Vol. 8.

Bibtex

@article{68253fcf26874ce39ab12d8db472152c,
title = "Super-resolution microscopy reveals functional organization of dopamine transporters into cholesterol and neuronal activity-dependent nanodomains",
abstract = "Dopamine regulates reward, cognition, and locomotor functions. By mediating rapid reuptake of extracellular dopamine, the dopamine transporter is critical for spatiotemporal control of dopaminergic neurotransmission. Here, we use super-resolution imaging to show that the dopamine transporter is dynamically sequestrated into cholesterol-dependent nanodomains in the plasma membrane of presynaptic varicosities and neuronal projections of dopaminergic neurons. Stochastic optical reconstruction microscopy reveals irregular dopamine transporter nanodomains (∼70 nm mean diameter) that were highly sensitive to cholesterol depletion. Live photoactivated localization microscopy shows a similar dopamine transporter membrane organization in live heterologous cells. In neurons, dual-color dSTORM shows that tyrosine hydroxylase and vesicular monoamine transporter-2 are distinctively localized adjacent to, but not overlapping with, the dopamine transporter nanodomains. The molecular organization of the dopamine transporter in nanodomains is reversibly reduced by short-term activation of NMDA-type ionotropic glutamate receptors, implicating dopamine transporter nanodomain distribution as a potential mechanism to modulate dopaminergic neurotransmission in response to excitatory input.",
author = "Troels Rahbek-Clemmensen and Lycas, {Matthew D.} and Simon Erlendsson and Jacob Eriksen and Mia Apuschkin and Frederik Vilhardt and Jorgensen, {Trine N.} and Hansen, {Freja H.} and Ulrik Gether",
year = "2017",
doi = "10.1038/s41467-017-00790-3",
language = "English",
volume = "8",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Super-resolution microscopy reveals functional organization of dopamine transporters into cholesterol and neuronal activity-dependent nanodomains

AU - Rahbek-Clemmensen, Troels

AU - Lycas, Matthew D.

AU - Erlendsson, Simon

AU - Eriksen, Jacob

AU - Apuschkin, Mia

AU - Vilhardt, Frederik

AU - Jorgensen, Trine N.

AU - Hansen, Freja H.

AU - Gether, Ulrik

PY - 2017

Y1 - 2017

N2 - Dopamine regulates reward, cognition, and locomotor functions. By mediating rapid reuptake of extracellular dopamine, the dopamine transporter is critical for spatiotemporal control of dopaminergic neurotransmission. Here, we use super-resolution imaging to show that the dopamine transporter is dynamically sequestrated into cholesterol-dependent nanodomains in the plasma membrane of presynaptic varicosities and neuronal projections of dopaminergic neurons. Stochastic optical reconstruction microscopy reveals irregular dopamine transporter nanodomains (∼70 nm mean diameter) that were highly sensitive to cholesterol depletion. Live photoactivated localization microscopy shows a similar dopamine transporter membrane organization in live heterologous cells. In neurons, dual-color dSTORM shows that tyrosine hydroxylase and vesicular monoamine transporter-2 are distinctively localized adjacent to, but not overlapping with, the dopamine transporter nanodomains. The molecular organization of the dopamine transporter in nanodomains is reversibly reduced by short-term activation of NMDA-type ionotropic glutamate receptors, implicating dopamine transporter nanodomain distribution as a potential mechanism to modulate dopaminergic neurotransmission in response to excitatory input.

AB - Dopamine regulates reward, cognition, and locomotor functions. By mediating rapid reuptake of extracellular dopamine, the dopamine transporter is critical for spatiotemporal control of dopaminergic neurotransmission. Here, we use super-resolution imaging to show that the dopamine transporter is dynamically sequestrated into cholesterol-dependent nanodomains in the plasma membrane of presynaptic varicosities and neuronal projections of dopaminergic neurons. Stochastic optical reconstruction microscopy reveals irregular dopamine transporter nanodomains (∼70 nm mean diameter) that were highly sensitive to cholesterol depletion. Live photoactivated localization microscopy shows a similar dopamine transporter membrane organization in live heterologous cells. In neurons, dual-color dSTORM shows that tyrosine hydroxylase and vesicular monoamine transporter-2 are distinctively localized adjacent to, but not overlapping with, the dopamine transporter nanodomains. The molecular organization of the dopamine transporter in nanodomains is reversibly reduced by short-term activation of NMDA-type ionotropic glutamate receptors, implicating dopamine transporter nanodomain distribution as a potential mechanism to modulate dopaminergic neurotransmission in response to excitatory input.

U2 - 10.1038/s41467-017-00790-3

DO - 10.1038/s41467-017-00790-3

M3 - Journal article

C2 - 28963530

VL - 8

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 740

ER -

ID: 184770387