Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins
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Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins. / Hawkins, Clare Louise; Rees, Martin D; Davies, Michael Jonathan.
In: FEBS Letters, Vol. 510, No. 1-2, 02.01.2002, p. 41-4.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins
AU - Hawkins, Clare Louise
AU - Rees, Martin D
AU - Davies, Michael Jonathan
PY - 2002/1/2
Y1 - 2002/1/2
N2 - Activated phagocytes generate both superoxide radicals via a respiratory burst, and HOCl via the concurrent release of the haem enzyme myeloperoxidase. Amine and amide functions on proteins and carbohydrates are major targets for HOCl, generating chloramines (RNHCl) and chloramides (RC(O)NClR'), which can accumulate to high concentrations (>100 microM). Here we show that superoxide radicals catalyse the decomposition of chloramines and chloramides to reactive nitrogen-centred radicals, and increase the extent of protein fragmentation compared to that observed with either superoxide radicals or HOCl, alone. This synergistic action may be of significance at sites of inflammation, where both superoxide radicals and chloramines/chloramides are formed simultaneously.
AB - Activated phagocytes generate both superoxide radicals via a respiratory burst, and HOCl via the concurrent release of the haem enzyme myeloperoxidase. Amine and amide functions on proteins and carbohydrates are major targets for HOCl, generating chloramines (RNHCl) and chloramides (RC(O)NClR'), which can accumulate to high concentrations (>100 microM). Here we show that superoxide radicals catalyse the decomposition of chloramines and chloramides to reactive nitrogen-centred radicals, and increase the extent of protein fragmentation compared to that observed with either superoxide radicals or HOCl, alone. This synergistic action may be of significance at sites of inflammation, where both superoxide radicals and chloramines/chloramides are formed simultaneously.
KW - Chloramines
KW - Drug Synergism
KW - Hypochlorous Acid
KW - Proteins
KW - Reactive Nitrogen Species
KW - Serum Albumin, Bovine
KW - Superoxides
M3 - Journal article
C2 - 11755528
VL - 510
SP - 41
EP - 44
JO - F E B S Letters
JF - F E B S Letters
SN - 0014-5793
IS - 1-2
ER -
ID: 138279041