Substrate specific stimulation of NEIL1 by WRN but not the other human RecQ helicases
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Substrate specific stimulation of NEIL1 by WRN but not the other human RecQ helicases. / Popuri, Venkateswarlu; Croteau, Deborah L; Bohr, Vilhelm A.
In: DNA Repair, Vol. 9, No. 6, 04.06.2010, p. 636-42.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Substrate specific stimulation of NEIL1 by WRN but not the other human RecQ helicases
AU - Popuri, Venkateswarlu
AU - Croteau, Deborah L
AU - Bohr, Vilhelm A
N1 - Copyright 2010. Published by Elsevier B.V.
PY - 2010/6/4
Y1 - 2010/6/4
N2 - NEIL1, the mammalian homolog of Escherichia coli endonuclease VIII, is a DNA glycosylase that repairs ring-fragmented purines, saturated pyrimidines and several oxidative lesions like 5-hydroxyuracil, 5-hydroxycytosine, etc. Previous studies from our laboratory have shown that Werner Syndrome protein (WRN), one of the five human RecQ helicases, stimulates NEIL1 DNA glycosylase activity on oxidative DNA lesions. The goal of this study was to extend this observation and analyze the interaction between NEIL1 and all five human RecQ helicases. The DNA substrate specificity of the interaction between WRN and NEIL1 was also analyzed. The results indicate that WRN is the only human RecQ helicase that stimulates NEIL1 DNA glycosylase activity, and that this stimulation requires a double-stranded DNA substrate.
AB - NEIL1, the mammalian homolog of Escherichia coli endonuclease VIII, is a DNA glycosylase that repairs ring-fragmented purines, saturated pyrimidines and several oxidative lesions like 5-hydroxyuracil, 5-hydroxycytosine, etc. Previous studies from our laboratory have shown that Werner Syndrome protein (WRN), one of the five human RecQ helicases, stimulates NEIL1 DNA glycosylase activity on oxidative DNA lesions. The goal of this study was to extend this observation and analyze the interaction between NEIL1 and all five human RecQ helicases. The DNA substrate specificity of the interaction between WRN and NEIL1 was also analyzed. The results indicate that WRN is the only human RecQ helicase that stimulates NEIL1 DNA glycosylase activity, and that this stimulation requires a double-stranded DNA substrate.
KW - Base Sequence
KW - DNA
KW - DNA Damage
KW - DNA Glycosylases
KW - DNA-(Apurinic or Apyrimidinic Site) Lyase
KW - Exodeoxyribonucleases
KW - Humans
KW - Oligodeoxyribonucleotides
KW - Oxidative Stress
KW - Protein Binding
KW - Pyrimidines
KW - RecQ Helicases
KW - Substrate Specificity
KW - Uracil
U2 - 10.1016/j.dnarep.2010.02.012
DO - 10.1016/j.dnarep.2010.02.012
M3 - Journal article
C2 - 20346739
VL - 9
SP - 636
EP - 642
JO - DNA Repair
JF - DNA Repair
SN - 1568-7864
IS - 6
ER -
ID: 33492016