Structural features of the Seneca Valley virus internal ribosome entry site (IRES) element: a picornavirus with a pestivirus-like IRES

Research output: Contribution to journal › Journal article › Research › peer-review

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Structural features of the Seneca Valley virus internal ribosome entry site (IRES) element : a picornavirus with a pestivirus-like IRES. / Willcocks, Margaret M; Locker, Nicolas; Gomwalk, Zarmwa; Royall, Elizabeth; Bakhshesh, Mehran; Belsham, Graham J; Idamakanti, Neeraja; Burroughs, Kevin D; Reddy, P Seshidhar; Hallenbeck, Paul L; Roberts, Lisa O.

In: Journal of Virology, Vol. 85, No. 9, 05.2011, p. 4452-61.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Willcocks, MM, Locker, N, Gomwalk, Z, Royall, E, Bakhshesh, M, Belsham, GJ, Idamakanti, N, Burroughs, KD, Reddy, PS, Hallenbeck, PL & Roberts, LO 2011, 'Structural features of the Seneca Valley virus internal ribosome entry site (IRES) element: a picornavirus with a pestivirus-like IRES', Journal of Virology, vol. 85, no. 9, pp. 4452-61. https://doi.org/10.1128/JVI.01107-10

APA

Willcocks, M. M., Locker, N., Gomwalk, Z., Royall, E., Bakhshesh, M., Belsham, G. J., Idamakanti, N., Burroughs, K. D., Reddy, P. S., Hallenbeck, P. L., & Roberts, L. O. (2011). Structural features of the Seneca Valley virus internal ribosome entry site (IRES) element: a picornavirus with a pestivirus-like IRES. Journal of Virology, 85(9), 4452-61. https://doi.org/10.1128/JVI.01107-10

Vancouver

Willcocks MM, Locker N, Gomwalk Z, Royall E, Bakhshesh M, Belsham GJ et al. Structural features of the Seneca Valley virus internal ribosome entry site (IRES) element: a picornavirus with a pestivirus-like IRES. Journal of Virology. 2011 May;85(9):4452-61. https://doi.org/10.1128/JVI.01107-10

Author

Willcocks, Margaret M ; Locker, Nicolas ; Gomwalk, Zarmwa ; Royall, Elizabeth ; Bakhshesh, Mehran ; Belsham, Graham J ; Idamakanti, Neeraja ; Burroughs, Kevin D ; Reddy, P Seshidhar ; Hallenbeck, Paul L ; Roberts, Lisa O. / Structural features of the Seneca Valley virus internal ribosome entry site (IRES) element : a picornavirus with a pestivirus-like IRES. In: Journal of Virology. 2011 ; Vol. 85, No. 9. pp. 4452-61.

Bibtex

@article{f65609eaa10a4c609fc91cabada8652d,
title = "Structural features of the Seneca Valley virus internal ribosome entry site (IRES) element: a picornavirus with a pestivirus-like IRES",
abstract = "The RNA genome of Seneca Valley virus (SVV), a recently identified picornavirus, contains an internal ribosome entry site (IRES) element which has structural and functional similarity to that from classical swine fever virus (CSFV) and hepatitis C virus, members of the Flaviviridae. The SVV IRES has an absolute requirement for the presence of a short region of virus-coding sequence to allow it to function either in cells or in rabbit reticulocyte lysate. The IRES activity does not require the translation initiation factor eIF4A or intact eIF4G. The predicted secondary structure indicates that the SVV IRES is more closely related to the CSFV IRES, including the presence of a bipartite IIId domain. Mutagenesis of the SVV IRES, coupled to functional assays, support the core elements of the IRES structure model, but surprisingly, deletion of the conserved IIId(2) domain had no effect on IRES activity, including 40S and eIF3 binding. This is the first example of a picornavirus IRES that is most closely related to the CSFV IRES and suggests the possibility of multiple, independent recombination events between the genomes of the Picornaviridae and Flaviviridae to give rise to similar IRES elements.",
keywords = "Animals, Cell Extracts, Cell Line, Classical Swine Fever Virus/genetics, DNA Mutational Analysis, Humans, Mutation, Nucleic Acid Conformation, Pestivirus/genetics, Picornaviridae/chemistry, Protein Biosynthesis, RNA, Viral/chemistry, Rabbits, Ribosomes/metabolism, Sequence Deletion",
author = "Willcocks, {Margaret M} and Nicolas Locker and Zarmwa Gomwalk and Elizabeth Royall and Mehran Bakhshesh and Belsham, {Graham J} and Neeraja Idamakanti and Burroughs, {Kevin D} and Reddy, {P Seshidhar} and Hallenbeck, {Paul L} and Roberts, {Lisa O}",
year = "2011",
month = may,
doi = "10.1128/JVI.01107-10",
language = "English",
volume = "85",
pages = "4452--61",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "9",

}

RIS

TY - JOUR

T1 - Structural features of the Seneca Valley virus internal ribosome entry site (IRES) element

T2 - a picornavirus with a pestivirus-like IRES

AU - Willcocks, Margaret M

AU - Locker, Nicolas

AU - Gomwalk, Zarmwa

AU - Royall, Elizabeth

AU - Bakhshesh, Mehran

AU - Belsham, Graham J

AU - Idamakanti, Neeraja

AU - Burroughs, Kevin D

AU - Reddy, P Seshidhar

AU - Hallenbeck, Paul L

AU - Roberts, Lisa O

PY - 2011/5

Y1 - 2011/5

N2 - The RNA genome of Seneca Valley virus (SVV), a recently identified picornavirus, contains an internal ribosome entry site (IRES) element which has structural and functional similarity to that from classical swine fever virus (CSFV) and hepatitis C virus, members of the Flaviviridae. The SVV IRES has an absolute requirement for the presence of a short region of virus-coding sequence to allow it to function either in cells or in rabbit reticulocyte lysate. The IRES activity does not require the translation initiation factor eIF4A or intact eIF4G. The predicted secondary structure indicates that the SVV IRES is more closely related to the CSFV IRES, including the presence of a bipartite IIId domain. Mutagenesis of the SVV IRES, coupled to functional assays, support the core elements of the IRES structure model, but surprisingly, deletion of the conserved IIId(2) domain had no effect on IRES activity, including 40S and eIF3 binding. This is the first example of a picornavirus IRES that is most closely related to the CSFV IRES and suggests the possibility of multiple, independent recombination events between the genomes of the Picornaviridae and Flaviviridae to give rise to similar IRES elements.

AB - The RNA genome of Seneca Valley virus (SVV), a recently identified picornavirus, contains an internal ribosome entry site (IRES) element which has structural and functional similarity to that from classical swine fever virus (CSFV) and hepatitis C virus, members of the Flaviviridae. The SVV IRES has an absolute requirement for the presence of a short region of virus-coding sequence to allow it to function either in cells or in rabbit reticulocyte lysate. The IRES activity does not require the translation initiation factor eIF4A or intact eIF4G. The predicted secondary structure indicates that the SVV IRES is more closely related to the CSFV IRES, including the presence of a bipartite IIId domain. Mutagenesis of the SVV IRES, coupled to functional assays, support the core elements of the IRES structure model, but surprisingly, deletion of the conserved IIId(2) domain had no effect on IRES activity, including 40S and eIF3 binding. This is the first example of a picornavirus IRES that is most closely related to the CSFV IRES and suggests the possibility of multiple, independent recombination events between the genomes of the Picornaviridae and Flaviviridae to give rise to similar IRES elements.

KW - Animals

KW - Cell Extracts

KW - Cell Line

KW - Classical Swine Fever Virus/genetics

KW - DNA Mutational Analysis

KW - Humans

KW - Mutation

KW - Nucleic Acid Conformation

KW - Pestivirus/genetics

KW - Picornaviridae/chemistry

KW - Protein Biosynthesis

KW - RNA, Viral/chemistry

KW - Rabbits

KW - Ribosomes/metabolism

KW - Sequence Deletion

U2 - 10.1128/JVI.01107-10

DO - 10.1128/JVI.01107-10

M3 - Journal article

C2 - 21325406

VL - 85

SP - 4452

EP - 4461

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 9

ER -

ID: 257917748