Significance of arginine 20 in the 2A protease for swine vesicular disease virus pathogenicity

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Significance of arginine 20 in the 2A protease for swine vesicular disease virus pathogenicity. / Inoue, Toru; Zhang, Zhidong; Wang, Leyuan; West, Laura; Bashiruddin, John B; Belsham, Graham J.

In: The Journal of general virology, Vol. 88, No. Pt 8, 08.2007, p. 2275-2279.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Inoue, T, Zhang, Z, Wang, L, West, L, Bashiruddin, JB & Belsham, GJ 2007, 'Significance of arginine 20 in the 2A protease for swine vesicular disease virus pathogenicity', The Journal of general virology, vol. 88, no. Pt 8, pp. 2275-2279. https://doi.org/10.1099/vir.0.82920-0

APA

Inoue, T., Zhang, Z., Wang, L., West, L., Bashiruddin, J. B., & Belsham, G. J. (2007). Significance of arginine 20 in the 2A protease for swine vesicular disease virus pathogenicity. The Journal of general virology, 88(Pt 8), 2275-2279. https://doi.org/10.1099/vir.0.82920-0

Vancouver

Inoue T, Zhang Z, Wang L, West L, Bashiruddin JB, Belsham GJ. Significance of arginine 20 in the 2A protease for swine vesicular disease virus pathogenicity. The Journal of general virology. 2007 Aug;88(Pt 8):2275-2279. https://doi.org/10.1099/vir.0.82920-0

Author

Inoue, Toru ; Zhang, Zhidong ; Wang, Leyuan ; West, Laura ; Bashiruddin, John B ; Belsham, Graham J. / Significance of arginine 20 in the 2A protease for swine vesicular disease virus pathogenicity. In: The Journal of general virology. 2007 ; Vol. 88, No. Pt 8. pp. 2275-2279.

Bibtex

@article{824f3c4dba3a4020baf28ffd6f6e1254,

title = "Significance of arginine 20 in the 2A protease for swine vesicular disease virus pathogenicity",

abstract = "Pathogenic and attenuated strains of swine vesicular disease virus (SVDV), an enterovirus, have been characterized previously and, by using chimeric infectious cDNA clones, the key determinants of pathogenicity in pigs have been mapped to the coding region for 1D-2A. Within this region, residue 20 of the 2A protease is particularly significant. Inoculation of pigs with mutant viruses containing single amino acid substitutions at this residue leads to the appearance of revertants, often containing an arginine at this position encoded by an AGA codon, one of six codons for this residue. The properties in pigs of two chimeric viruses, each with an arginine residue at this position but encoded by different codons, have been investigated in parallel with the parental pathogenic and attenuated strains. Presence of the arginine residue, but not of the AGA codon, is essential for induction of high viraemia and appearance of significant disease.",

keywords = "Animals, Arginine/physiology, Cysteine Endopeptidases/chemistry, Enterovirus B, Human/enzymology, Swine, Swine Vesicular Disease/virology, Viral Proteins/chemistry, Virulence",

author = "Toru Inoue and Zhidong Zhang and Leyuan Wang and Laura West and Bashiruddin, {John B} and Belsham, {Graham J}",

year = "2007",

month = aug,

doi = "10.1099/vir.0.82920-0",

language = "English",

volume = "88",

pages = "2275--2279",

journal = "Journal of General Virology",

issn = "0022-1317",

publisher = "Society for General Microbiology",

number = "Pt 8",

}

RIS

TY - JOUR

T1 - Significance of arginine 20 in the 2A protease for swine vesicular disease virus pathogenicity

AU - Inoue, Toru

AU - Zhang, Zhidong

AU - Wang, Leyuan

AU - West, Laura

AU - Bashiruddin, John B

AU - Belsham, Graham J

PY - 2007/8

Y1 - 2007/8

N2 - Pathogenic and attenuated strains of swine vesicular disease virus (SVDV), an enterovirus, have been characterized previously and, by using chimeric infectious cDNA clones, the key determinants of pathogenicity in pigs have been mapped to the coding region for 1D-2A. Within this region, residue 20 of the 2A protease is particularly significant. Inoculation of pigs with mutant viruses containing single amino acid substitutions at this residue leads to the appearance of revertants, often containing an arginine at this position encoded by an AGA codon, one of six codons for this residue. The properties in pigs of two chimeric viruses, each with an arginine residue at this position but encoded by different codons, have been investigated in parallel with the parental pathogenic and attenuated strains. Presence of the arginine residue, but not of the AGA codon, is essential for induction of high viraemia and appearance of significant disease.

AB - Pathogenic and attenuated strains of swine vesicular disease virus (SVDV), an enterovirus, have been characterized previously and, by using chimeric infectious cDNA clones, the key determinants of pathogenicity in pigs have been mapped to the coding region for 1D-2A. Within this region, residue 20 of the 2A protease is particularly significant. Inoculation of pigs with mutant viruses containing single amino acid substitutions at this residue leads to the appearance of revertants, often containing an arginine at this position encoded by an AGA codon, one of six codons for this residue. The properties in pigs of two chimeric viruses, each with an arginine residue at this position but encoded by different codons, have been investigated in parallel with the parental pathogenic and attenuated strains. Presence of the arginine residue, but not of the AGA codon, is essential for induction of high viraemia and appearance of significant disease.

KW - Animals

KW - Arginine/physiology

KW - Cysteine Endopeptidases/chemistry

KW - Enterovirus B, Human/enzymology

KW - Swine

KW - Swine Vesicular Disease/virology

KW - Viral Proteins/chemistry

KW - Virulence

U2 - 10.1099/vir.0.82920-0

DO - 10.1099/vir.0.82920-0

M3 - Journal article

C2 - 17622632

VL - 88

SP - 2275

EP - 2279

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - Pt 8

ER -

ID: 257918688