Serine34 phosphorylation of RHO guanine dissociation inhibitor (RHOGDI{alpha}) links signaling from conventional protein kinase C to RHO GTPase in cell adhesion
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Serine34 phosphorylation of RHO guanine dissociation inhibitor (RHOGDI{alpha}) links signaling from conventional protein kinase C to RHO GTPase in cell adhesion. / Dovas, Athanassios; Choi, Youngsil; Yoneda, Atsuko; Multhaupt, Hinke A B; Kwon, Seung-Hae; Kang, Dongmin; Oh, Eok-Soo; Couchman, John R.
In: Journal of Biological Chemistry, Vol. 285, No. 30, 15.05.2010, p. 23296-308.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Serine34 phosphorylation of RHO guanine dissociation inhibitor (RHOGDI{alpha}) links signaling from conventional protein kinase C to RHO GTPase in cell adhesion
AU - Dovas, Athanassios
AU - Choi, Youngsil
AU - Yoneda, Atsuko
AU - Multhaupt, Hinke A B
AU - Kwon, Seung-Hae
AU - Kang, Dongmin
AU - Oh, Eok-Soo
AU - Couchman, John R
PY - 2010/5/15
Y1 - 2010/5/15
N2 - Protein kinase Calpha (PKCalpha) is an essential serine/threonine kinase regulating many signaling networks. At cell adhesion sites, PKCalpha can impact the actin cytoskeleton through its influence on RhoGTPases but the intermediate steps are not well known. One important regulator of RhoGTPase function is the multifunctional guanine nucleotide dissociation inhibitor RhoGDIalpha that sequesters several related RhoGTPases in an inactive form, but may also target them through interactions with actin-associated proteins. Here it is demonstrated that PKCalpha phosphorylates RhoGDIalpha on serine 34, resulting in a specific decrease in affinity for RhoA, but not Rac1 or cdc42. The mechanism of RhoGDIalpha phosphorylation is distinct, requiring PKCalpha and phosphatidylinositol 4,5 bisphosphate, consistent with recent evidence that the inositide can activate, localize and orient PKCalpha in membranes. Phosphospecific antibodies reveal endogenous phosphorylation in several cell types that is sensitive to adhesion events triggered, for example, by hepatocyte growth factor. Phosphorylation is also sensitive to PKC inhibition. Together with FRET microscopy sensing GTP-RhoA levels, the data reveal a common pathway in cell adhesion linking two essential mediators, PKCalpha and RhoA.
AB - Protein kinase Calpha (PKCalpha) is an essential serine/threonine kinase regulating many signaling networks. At cell adhesion sites, PKCalpha can impact the actin cytoskeleton through its influence on RhoGTPases but the intermediate steps are not well known. One important regulator of RhoGTPase function is the multifunctional guanine nucleotide dissociation inhibitor RhoGDIalpha that sequesters several related RhoGTPases in an inactive form, but may also target them through interactions with actin-associated proteins. Here it is demonstrated that PKCalpha phosphorylates RhoGDIalpha on serine 34, resulting in a specific decrease in affinity for RhoA, but not Rac1 or cdc42. The mechanism of RhoGDIalpha phosphorylation is distinct, requiring PKCalpha and phosphatidylinositol 4,5 bisphosphate, consistent with recent evidence that the inositide can activate, localize and orient PKCalpha in membranes. Phosphospecific antibodies reveal endogenous phosphorylation in several cell types that is sensitive to adhesion events triggered, for example, by hepatocyte growth factor. Phosphorylation is also sensitive to PKC inhibition. Together with FRET microscopy sensing GTP-RhoA levels, the data reveal a common pathway in cell adhesion linking two essential mediators, PKCalpha and RhoA.
U2 - 10.1074/jbc.M109.098129
DO - 10.1074/jbc.M109.098129
M3 - Journal article
C2 - 20472934
VL - 285
SP - 23296
EP - 23308
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 30
ER -
ID: 19977473