Repair of DNA damage induced by anthanthrene, a polycyclic aromatic hydrocarbon (PAH) without bay or fjord regions

Research output: Contribution to journalJournal articlepeer-review

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Repair of DNA damage induced by anthanthrene, a polycyclic aromatic hydrocarbon (PAH) without bay or fjord regions. / Madsen, Claus Desler; Johannessen, Christian; Rasmussen, Lene Juel.

In: Chemico-Biological Interactions, Vol. 177, No. 3, 2009, p. 212-7.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Madsen, CD, Johannessen, C & Rasmussen, LJ 2009, 'Repair of DNA damage induced by anthanthrene, a polycyclic aromatic hydrocarbon (PAH) without bay or fjord regions', Chemico-Biological Interactions, vol. 177, no. 3, pp. 212-7. https://doi.org/10.1016/j.cbi.2008.10.056

APA

Madsen, C. D., Johannessen, C., & Rasmussen, L. J. (2009). Repair of DNA damage induced by anthanthrene, a polycyclic aromatic hydrocarbon (PAH) without bay or fjord regions. Chemico-Biological Interactions, 177(3), 212-7. https://doi.org/10.1016/j.cbi.2008.10.056

Vancouver

Madsen CD, Johannessen C, Rasmussen LJ. Repair of DNA damage induced by anthanthrene, a polycyclic aromatic hydrocarbon (PAH) without bay or fjord regions. Chemico-Biological Interactions. 2009;177(3):212-7. https://doi.org/10.1016/j.cbi.2008.10.056

Author

Madsen, Claus Desler ; Johannessen, Christian ; Rasmussen, Lene Juel. / Repair of DNA damage induced by anthanthrene, a polycyclic aromatic hydrocarbon (PAH) without bay or fjord regions. In: Chemico-Biological Interactions. 2009 ; Vol. 177, No. 3. pp. 212-7.

Bibtex

@article{9e87ce10962911df928f000ea68e967b,
title = "Repair of DNA damage induced by anthanthrene, a polycyclic aromatic hydrocarbon (PAH) without bay or fjord regions",
abstract = "Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants, formed during incomplete burning of coal, oil and gas. Several PAHs have carcinogenic and mutagenic potencies, but these compounds must be activated in order to exert their mutagenic effects. One of the principal pathways proposed for metabolic activation of PAHs involves the cytochrome P450 enzymes. The DNA damaging potential of cytochrome P450-activated PAHs is generally associated with their bay and fjord regions, and the DNA repair response of PAHs containing such regions has been thoroughly studied. However, little is known about the repair of DNA damage resulting from metabolites from PAHs without bay and fjord regions. We have investigated the six-ringed PAH anthanthrene (dibenzo[def,mno]chrysene), which does not posses bay or fjord motifs. We analyzed the repair profile of human cell extracts and of cell cultures in response to DNA damage induced by cytochrome P450-activated anthanthrene. In cell extracts, functional nucleotide excision repair (NER) and mismatch repair (MMR) activities were necessary to trigger a response to anthanthrene metabolite-induced DNA damage. In cell cultures, NER was responsible for the repair of anthanthrene metabolite-induced DNA damage. However, when the NER pathway was inactivated, a residual repair pathway performed the DNA repair.",
author = "Madsen, {Claus Desler} and Christian Johannessen and Rasmussen, {Lene Juel}",
note = "Keywords: Cell Line, Transformed; Comet Assay; DNA; DNA Damage; DNA Repair; Humans; Polycyclic Compounds; Water Pollutants, Chemical",
year = "2009",
doi = "10.1016/j.cbi.2008.10.056",
language = "English",
volume = "177",
pages = "212--7",
journal = "Chemico-Biological Interactions",
issn = "0009-2797",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - Repair of DNA damage induced by anthanthrene, a polycyclic aromatic hydrocarbon (PAH) without bay or fjord regions

AU - Madsen, Claus Desler

AU - Johannessen, Christian

AU - Rasmussen, Lene Juel

N1 - Keywords: Cell Line, Transformed; Comet Assay; DNA; DNA Damage; DNA Repair; Humans; Polycyclic Compounds; Water Pollutants, Chemical

PY - 2009

Y1 - 2009

N2 - Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants, formed during incomplete burning of coal, oil and gas. Several PAHs have carcinogenic and mutagenic potencies, but these compounds must be activated in order to exert their mutagenic effects. One of the principal pathways proposed for metabolic activation of PAHs involves the cytochrome P450 enzymes. The DNA damaging potential of cytochrome P450-activated PAHs is generally associated with their bay and fjord regions, and the DNA repair response of PAHs containing such regions has been thoroughly studied. However, little is known about the repair of DNA damage resulting from metabolites from PAHs without bay and fjord regions. We have investigated the six-ringed PAH anthanthrene (dibenzo[def,mno]chrysene), which does not posses bay or fjord motifs. We analyzed the repair profile of human cell extracts and of cell cultures in response to DNA damage induced by cytochrome P450-activated anthanthrene. In cell extracts, functional nucleotide excision repair (NER) and mismatch repair (MMR) activities were necessary to trigger a response to anthanthrene metabolite-induced DNA damage. In cell cultures, NER was responsible for the repair of anthanthrene metabolite-induced DNA damage. However, when the NER pathway was inactivated, a residual repair pathway performed the DNA repair.

AB - Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants, formed during incomplete burning of coal, oil and gas. Several PAHs have carcinogenic and mutagenic potencies, but these compounds must be activated in order to exert their mutagenic effects. One of the principal pathways proposed for metabolic activation of PAHs involves the cytochrome P450 enzymes. The DNA damaging potential of cytochrome P450-activated PAHs is generally associated with their bay and fjord regions, and the DNA repair response of PAHs containing such regions has been thoroughly studied. However, little is known about the repair of DNA damage resulting from metabolites from PAHs without bay and fjord regions. We have investigated the six-ringed PAH anthanthrene (dibenzo[def,mno]chrysene), which does not posses bay or fjord motifs. We analyzed the repair profile of human cell extracts and of cell cultures in response to DNA damage induced by cytochrome P450-activated anthanthrene. In cell extracts, functional nucleotide excision repair (NER) and mismatch repair (MMR) activities were necessary to trigger a response to anthanthrene metabolite-induced DNA damage. In cell cultures, NER was responsible for the repair of anthanthrene metabolite-induced DNA damage. However, when the NER pathway was inactivated, a residual repair pathway performed the DNA repair.

U2 - 10.1016/j.cbi.2008.10.056

DO - 10.1016/j.cbi.2008.10.056

M3 - Journal article

C2 - 19046955

VL - 177

SP - 212

EP - 217

JO - Chemico-Biological Interactions

JF - Chemico-Biological Interactions

SN - 0009-2797

IS - 3

ER -

ID: 20990780