Regulation of laminin γ2 expression by CDX2 in colonic epithelial cells is impaired during active inflammation

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Regulation of laminin γ2 expression by CDX2 in colonic epithelial cells is impaired during active inflammation. / Coskun, Mehmet; Soendergaard, Christoffer; Joergensen, Steffen; Dahlgaard, Katja; Riis, Lene Buhl; Nielsen, Ole Haagen; Sandelin, Albin Gustav; Troelsen, Jesper Thorvald.

In: Journal of Cellular Biochemistry, Vol. 118, No. 2, 2017, p. 298-307.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Coskun, M, Soendergaard, C, Joergensen, S, Dahlgaard, K, Riis, LB, Nielsen, OH, Sandelin, AG & Troelsen, JT 2017, 'Regulation of laminin γ2 expression by CDX2 in colonic epithelial cells is impaired during active inflammation', Journal of Cellular Biochemistry, vol. 118, no. 2, pp. 298-307. https://doi.org/10.1002/jcb.25636

APA

Coskun, M., Soendergaard, C., Joergensen, S., Dahlgaard, K., Riis, L. B., Nielsen, O. H., ... Troelsen, J. T. (2017). Regulation of laminin γ2 expression by CDX2 in colonic epithelial cells is impaired during active inflammation. Journal of Cellular Biochemistry, 118(2), 298-307. https://doi.org/10.1002/jcb.25636

Vancouver

Coskun M, Soendergaard C, Joergensen S, Dahlgaard K, Riis LB, Nielsen OH et al. Regulation of laminin γ2 expression by CDX2 in colonic epithelial cells is impaired during active inflammation. Journal of Cellular Biochemistry. 2017;118(2):298-307. https://doi.org/10.1002/jcb.25636

Author

Coskun, Mehmet ; Soendergaard, Christoffer ; Joergensen, Steffen ; Dahlgaard, Katja ; Riis, Lene Buhl ; Nielsen, Ole Haagen ; Sandelin, Albin Gustav ; Troelsen, Jesper Thorvald. / Regulation of laminin γ2 expression by CDX2 in colonic epithelial cells is impaired during active inflammation. In: Journal of Cellular Biochemistry. 2017 ; Vol. 118, No. 2. pp. 298-307.

Bibtex

@article{0c149eb2398640d8aa5d75e2c1888270,
title = "Regulation of laminin γ2 expression by CDX2 in colonic epithelial cells is impaired during active inflammation",
abstract = "The expression of Caudal-related homeobox transcription factor 2 (CDX2) is impaired by tumor necrosis factor-α (TNF-α)-mediated activation of nuclear factor-κB (NF-κB) in ulcerative colitis (UC). Laminin subunit γ2 (LAMC2) is an epithelial basement membrane protein implicated in cell migration, proliferation, differentiation, as well as tumor invasion and intestinal inflammation, and its expression is enhanced by TNF-α in a NF-κB-dependent regulation of the recently identified LAMC2 enhancer. The aim was to determine whether CDX2 is involved in the basal regulation of LAMC2 in epithelial cells and to assess the influence of inflammation. Transcriptional regulation of LAMC2 was examined by reporter gene assays, overexpression, and shRNA-mediated knock-down of CDX2. CDX2-DNA interactions were assessed by chromatin immunoprecipitation on Caco-2 cells without or with TNF-α, as well as in purified colonic human epithelial cells. Immunohistochemical staining and quantitative reverse-transcription polymerase chain reaction analyses were used to measure the expression of CDX2 and LAMC2 in colonic biopsies from healthy controls and patients with UC. These data indicate that CDX2 directly regulates LAMC2 gene expression through interaction with elements in the LAMC2 promoter region. We further revealed an inverse effect of inflammation on CDX2 and LAMC2. The data presented provide a novel insight into how CDX2 is implicated in the transcriptional regulation of LAMC2 in intestinal epithelial cells, a function that is impaired during mucosal inflammation where a high level of TNF-α is present. J. Cell. Biochem. 9999: 1-10, 2016. {\circledC} 2016 Wiley Periodicals, Inc.",
author = "Mehmet Coskun and Christoffer Soendergaard and Steffen Joergensen and Katja Dahlgaard and Riis, {Lene Buhl} and Nielsen, {Ole Haagen} and Sandelin, {Albin Gustav} and Troelsen, {Jesper Thorvald}",
note = "{\circledC} 2016 Wiley Periodicals, Inc.",
year = "2017",
doi = "10.1002/jcb.25636",
language = "English",
volume = "118",
pages = "298--307",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "JohnWiley & Sons, Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Regulation of laminin γ2 expression by CDX2 in colonic epithelial cells is impaired during active inflammation

AU - Coskun, Mehmet

AU - Soendergaard, Christoffer

AU - Joergensen, Steffen

AU - Dahlgaard, Katja

AU - Riis, Lene Buhl

AU - Nielsen, Ole Haagen

AU - Sandelin, Albin Gustav

AU - Troelsen, Jesper Thorvald

N1 - © 2016 Wiley Periodicals, Inc.

PY - 2017

Y1 - 2017

N2 - The expression of Caudal-related homeobox transcription factor 2 (CDX2) is impaired by tumor necrosis factor-α (TNF-α)-mediated activation of nuclear factor-κB (NF-κB) in ulcerative colitis (UC). Laminin subunit γ2 (LAMC2) is an epithelial basement membrane protein implicated in cell migration, proliferation, differentiation, as well as tumor invasion and intestinal inflammation, and its expression is enhanced by TNF-α in a NF-κB-dependent regulation of the recently identified LAMC2 enhancer. The aim was to determine whether CDX2 is involved in the basal regulation of LAMC2 in epithelial cells and to assess the influence of inflammation. Transcriptional regulation of LAMC2 was examined by reporter gene assays, overexpression, and shRNA-mediated knock-down of CDX2. CDX2-DNA interactions were assessed by chromatin immunoprecipitation on Caco-2 cells without or with TNF-α, as well as in purified colonic human epithelial cells. Immunohistochemical staining and quantitative reverse-transcription polymerase chain reaction analyses were used to measure the expression of CDX2 and LAMC2 in colonic biopsies from healthy controls and patients with UC. These data indicate that CDX2 directly regulates LAMC2 gene expression through interaction with elements in the LAMC2 promoter region. We further revealed an inverse effect of inflammation on CDX2 and LAMC2. The data presented provide a novel insight into how CDX2 is implicated in the transcriptional regulation of LAMC2 in intestinal epithelial cells, a function that is impaired during mucosal inflammation where a high level of TNF-α is present. J. Cell. Biochem. 9999: 1-10, 2016. © 2016 Wiley Periodicals, Inc.

AB - The expression of Caudal-related homeobox transcription factor 2 (CDX2) is impaired by tumor necrosis factor-α (TNF-α)-mediated activation of nuclear factor-κB (NF-κB) in ulcerative colitis (UC). Laminin subunit γ2 (LAMC2) is an epithelial basement membrane protein implicated in cell migration, proliferation, differentiation, as well as tumor invasion and intestinal inflammation, and its expression is enhanced by TNF-α in a NF-κB-dependent regulation of the recently identified LAMC2 enhancer. The aim was to determine whether CDX2 is involved in the basal regulation of LAMC2 in epithelial cells and to assess the influence of inflammation. Transcriptional regulation of LAMC2 was examined by reporter gene assays, overexpression, and shRNA-mediated knock-down of CDX2. CDX2-DNA interactions were assessed by chromatin immunoprecipitation on Caco-2 cells without or with TNF-α, as well as in purified colonic human epithelial cells. Immunohistochemical staining and quantitative reverse-transcription polymerase chain reaction analyses were used to measure the expression of CDX2 and LAMC2 in colonic biopsies from healthy controls and patients with UC. These data indicate that CDX2 directly regulates LAMC2 gene expression through interaction with elements in the LAMC2 promoter region. We further revealed an inverse effect of inflammation on CDX2 and LAMC2. The data presented provide a novel insight into how CDX2 is implicated in the transcriptional regulation of LAMC2 in intestinal epithelial cells, a function that is impaired during mucosal inflammation where a high level of TNF-α is present. J. Cell. Biochem. 9999: 1-10, 2016. © 2016 Wiley Periodicals, Inc.

U2 - 10.1002/jcb.25636

DO - 10.1002/jcb.25636

M3 - Journal article

C2 - 27333824

VL - 118

SP - 298

EP - 307

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 2

ER -

ID: 166020245