Reduced metal ion concentrations in atherosclerotic plaques from subjects with type 2 diabetes mellitus
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Reduced metal ion concentrations in atherosclerotic plaques from subjects with type 2 diabetes mellitus. / Stadler, Nadina; Heeneman, Sylvia; Vöö, Stefan; Stanley, Naomi; Giles, Gregory I; Gang, Bevan P; Croft, Kevin D; Mori, Trevor A; Vacata, Vladimir; Daemen, Mat J A P; Waltenberger, Johannes; Davies, Michael Jonathan.
In: Atherosclerosis, Vol. 222, No. 2, 06.2012, p. 512-8.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Reduced metal ion concentrations in atherosclerotic plaques from subjects with type 2 diabetes mellitus
AU - Stadler, Nadina
AU - Heeneman, Sylvia
AU - Vöö, Stefan
AU - Stanley, Naomi
AU - Giles, Gregory I
AU - Gang, Bevan P
AU - Croft, Kevin D
AU - Mori, Trevor A
AU - Vacata, Vladimir
AU - Daemen, Mat J A P
AU - Waltenberger, Johannes
AU - Davies, Michael Jonathan
N1 - Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
PY - 2012/6
Y1 - 2012/6
N2 - AIMS: Transition metal ions have been implicated in atherosclerosis. The goal of this study was to investigate whether metal ion levels were higher in people with diabetes, in view of their increased risk of aggravated atherosclerosis.METHODS AND RESULTS: Absolute concentrations of iron, copper, zinc and calcium, and products of protein and lipid oxidation were quantified in atherosclerotic lesions from subjects with (T2DM, n=27), without Type 2 diabetes (nonDM, n=22), or hyperglycaemia (HG, n=17). Iron (P<0.05), zinc (P<0.01) and calcium (P=0.01) were lower in T2DM compared to nonDM subjects. Copper levels were comparable. A strong correlation (r=0.618; P<0.001) between EPR-detectable and total iron in nonDM patients was not seen in T2DM. X-ray fluorescence microscopy revealed "hot spots" of iron in both T2DM and nonDM. Calcium and zinc co-localised and levels correlated strongly. F(2)-isoprostanes (P<0.05) and di-Tyr/Tyr ratio (P<0.025), oxidative damage markers were decreased in T2DM compared to nonDM, or HG.CONCLUSION: Advanced atherosclerotic lesions from T2DM subjects unexpectedly contained lower levels of transition metal ions, and protein and lipid oxidation products, compared to nonDM and HG. These data do not support the hypothesis that elevated metal ion levels may be a major causative factor in the aggravated atherosclerosis observed in T2DM patients.
AB - AIMS: Transition metal ions have been implicated in atherosclerosis. The goal of this study was to investigate whether metal ion levels were higher in people with diabetes, in view of their increased risk of aggravated atherosclerosis.METHODS AND RESULTS: Absolute concentrations of iron, copper, zinc and calcium, and products of protein and lipid oxidation were quantified in atherosclerotic lesions from subjects with (T2DM, n=27), without Type 2 diabetes (nonDM, n=22), or hyperglycaemia (HG, n=17). Iron (P<0.05), zinc (P<0.01) and calcium (P=0.01) were lower in T2DM compared to nonDM subjects. Copper levels were comparable. A strong correlation (r=0.618; P<0.001) between EPR-detectable and total iron in nonDM patients was not seen in T2DM. X-ray fluorescence microscopy revealed "hot spots" of iron in both T2DM and nonDM. Calcium and zinc co-localised and levels correlated strongly. F(2)-isoprostanes (P<0.05) and di-Tyr/Tyr ratio (P<0.025), oxidative damage markers were decreased in T2DM compared to nonDM, or HG.CONCLUSION: Advanced atherosclerotic lesions from T2DM subjects unexpectedly contained lower levels of transition metal ions, and protein and lipid oxidation products, compared to nonDM and HG. These data do not support the hypothesis that elevated metal ion levels may be a major causative factor in the aggravated atherosclerosis observed in T2DM patients.
KW - Autopsy
KW - Calcium
KW - Carotid Arteries
KW - Carotid Artery Diseases
KW - Copper
KW - Diabetes Mellitus, Type 2
KW - Diabetic Angiopathies
KW - Down-Regulation
KW - Electron Spin Resonance Spectroscopy
KW - F2-Isoprostanes
KW - Female
KW - Humans
KW - Iron
KW - Lipids
KW - Male
KW - Mass Spectrometry
KW - Metals
KW - Microscopy, Fluorescence
KW - Netherlands
KW - Oxidation-Reduction
KW - Plaque, Atherosclerotic
KW - Zinc
U2 - 10.1016/j.atherosclerosis.2012.03.015
DO - 10.1016/j.atherosclerosis.2012.03.015
M3 - Journal article
C2 - 22521900
VL - 222
SP - 512
EP - 518
JO - Atherosclerosis
JF - Atherosclerosis
SN - 0021-9150
IS - 2
ER -
ID: 128975076