Reconstruction of the transmission history of RNA virus outbreaks using full genome sequences: foot-and-mouth disease virus in Bulgaria in 2011

Research output: Contribution to journalJournal articleResearchpeer-review

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Reconstruction of the transmission history of RNA virus outbreaks using full genome sequences : foot-and-mouth disease virus in Bulgaria in 2011. / Valdazo-González, Begoña; Polihronova, Lilyana; Alexandrov, Tsviatko; Normann, Preben; Knowles, Nick J; Hammond, Jef M; Georgiev, Georgi K; Özyörük, Fuat; Sumption, Keith J; Belsham, Graham J; King, Donald P.

In: PLoS ONE, Vol. 7, No. 11, 2012, p. e49650.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Valdazo-González, B, Polihronova, L, Alexandrov, T, Normann, P, Knowles, NJ, Hammond, JM, Georgiev, GK, Özyörük, F, Sumption, KJ, Belsham, GJ & King, DP 2012, 'Reconstruction of the transmission history of RNA virus outbreaks using full genome sequences: foot-and-mouth disease virus in Bulgaria in 2011', PLoS ONE, vol. 7, no. 11, pp. e49650. https://doi.org/10.1371/journal.pone.0049650

APA

Valdazo-González, B., Polihronova, L., Alexandrov, T., Normann, P., Knowles, N. J., Hammond, J. M., Georgiev, G. K., Özyörük, F., Sumption, K. J., Belsham, G. J., & King, D. P. (2012). Reconstruction of the transmission history of RNA virus outbreaks using full genome sequences: foot-and-mouth disease virus in Bulgaria in 2011. PLoS ONE, 7(11), e49650. https://doi.org/10.1371/journal.pone.0049650

Vancouver

Valdazo-González B, Polihronova L, Alexandrov T, Normann P, Knowles NJ, Hammond JM et al. Reconstruction of the transmission history of RNA virus outbreaks using full genome sequences: foot-and-mouth disease virus in Bulgaria in 2011. PLoS ONE. 2012;7(11):e49650. https://doi.org/10.1371/journal.pone.0049650

Author

Valdazo-González, Begoña ; Polihronova, Lilyana ; Alexandrov, Tsviatko ; Normann, Preben ; Knowles, Nick J ; Hammond, Jef M ; Georgiev, Georgi K ; Özyörük, Fuat ; Sumption, Keith J ; Belsham, Graham J ; King, Donald P. / Reconstruction of the transmission history of RNA virus outbreaks using full genome sequences : foot-and-mouth disease virus in Bulgaria in 2011. In: PLoS ONE. 2012 ; Vol. 7, No. 11. pp. e49650.

Bibtex

@article{39a938dfd8694c58a0bbcb02680d4e99,
title = "Reconstruction of the transmission history of RNA virus outbreaks using full genome sequences: foot-and-mouth disease virus in Bulgaria in 2011",
abstract = "Improvements to sequencing protocols and the development of computational phylogenetics have opened up opportunities to study the rapid evolution of RNA viruses in real time. In practical terms, these results can be combined with field data in order to reconstruct spatiotemporal scenarios that describe the origin and transmission pathways of viruses during an epidemic. In the case of notifiable diseases, such as foot-and-mouth disease (FMD), these analyses provide important insights into the epidemiology of field outbreaks that can support disease control programmes. This study reconstructs the origin and transmission history of the FMD outbreaks which occurred during 2011 in Burgas Province, Bulgaria, a country that had been previously FMD-free-without-vaccination since 1996. Nineteen full genome sequences (FGS) of FMD virus (FMDV) were generated and analysed, including eight representative viruses from all of the virus-positive outbreaks of the disease in the country and 11 closely-related contemporary viruses from countries in the region where FMD is endemic (Turkey and Israel). All Bulgarian sequences shared a single putative common ancestor which was closely related to the index case identified in wild boar. The closest relative from outside of Bulgaria was a FMDV collected during 2010 in Bursa (Anatolia, Turkey). Within Bulgaria, two discrete genetic clusters were detected that corresponded to two episodes of outbreaks that occurred during January and March-April 2011. The number of nucleotide substitutions that were present between, and within, these separate clusters provided evidence that undetected FMDV infection had occurred. These conclusions are supported by laboratory data that subsequently identified three additional FMDV-infected livestock premises by serosurveillance, as well as a number of antibody positive wild boar on both sides of the border with Turkish Thrace. This study highlights how FGS analysis can be used as an effective on-the-spot tool to support and help direct epidemiological investigations of field outbreaks.",
keywords = "Animals, Base Sequence, Bulgaria/epidemiology, Cattle, Cattle Diseases/epidemiology, Disease Outbreaks, Epidemiological Monitoring, Evolution, Molecular, Foot-and-Mouth Disease/epidemiology, Foot-and-Mouth Disease Virus/genetics, Genome, Viral, Israel/epidemiology, Multigene Family, Phylogeny, Phylogeography, Sus scrofa, Swine, Swine Diseases/epidemiology, Turkey/epidemiology",
author = "Bego{\~n}a Valdazo-Gonz{\'a}lez and Lilyana Polihronova and Tsviatko Alexandrov and Preben Normann and Knowles, {Nick J} and Hammond, {Jef M} and Georgiev, {Georgi K} and Fuat {\"O}zy{\"o}r{\"u}k and Sumption, {Keith J} and Belsham, {Graham J} and King, {Donald P}",
year = "2012",
doi = "10.1371/journal.pone.0049650",
language = "English",
volume = "7",
pages = "e49650",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

RIS

TY - JOUR

T1 - Reconstruction of the transmission history of RNA virus outbreaks using full genome sequences

T2 - foot-and-mouth disease virus in Bulgaria in 2011

AU - Valdazo-González, Begoña

AU - Polihronova, Lilyana

AU - Alexandrov, Tsviatko

AU - Normann, Preben

AU - Knowles, Nick J

AU - Hammond, Jef M

AU - Georgiev, Georgi K

AU - Özyörük, Fuat

AU - Sumption, Keith J

AU - Belsham, Graham J

AU - King, Donald P

PY - 2012

Y1 - 2012

N2 - Improvements to sequencing protocols and the development of computational phylogenetics have opened up opportunities to study the rapid evolution of RNA viruses in real time. In practical terms, these results can be combined with field data in order to reconstruct spatiotemporal scenarios that describe the origin and transmission pathways of viruses during an epidemic. In the case of notifiable diseases, such as foot-and-mouth disease (FMD), these analyses provide important insights into the epidemiology of field outbreaks that can support disease control programmes. This study reconstructs the origin and transmission history of the FMD outbreaks which occurred during 2011 in Burgas Province, Bulgaria, a country that had been previously FMD-free-without-vaccination since 1996. Nineteen full genome sequences (FGS) of FMD virus (FMDV) were generated and analysed, including eight representative viruses from all of the virus-positive outbreaks of the disease in the country and 11 closely-related contemporary viruses from countries in the region where FMD is endemic (Turkey and Israel). All Bulgarian sequences shared a single putative common ancestor which was closely related to the index case identified in wild boar. The closest relative from outside of Bulgaria was a FMDV collected during 2010 in Bursa (Anatolia, Turkey). Within Bulgaria, two discrete genetic clusters were detected that corresponded to two episodes of outbreaks that occurred during January and March-April 2011. The number of nucleotide substitutions that were present between, and within, these separate clusters provided evidence that undetected FMDV infection had occurred. These conclusions are supported by laboratory data that subsequently identified three additional FMDV-infected livestock premises by serosurveillance, as well as a number of antibody positive wild boar on both sides of the border with Turkish Thrace. This study highlights how FGS analysis can be used as an effective on-the-spot tool to support and help direct epidemiological investigations of field outbreaks.

AB - Improvements to sequencing protocols and the development of computational phylogenetics have opened up opportunities to study the rapid evolution of RNA viruses in real time. In practical terms, these results can be combined with field data in order to reconstruct spatiotemporal scenarios that describe the origin and transmission pathways of viruses during an epidemic. In the case of notifiable diseases, such as foot-and-mouth disease (FMD), these analyses provide important insights into the epidemiology of field outbreaks that can support disease control programmes. This study reconstructs the origin and transmission history of the FMD outbreaks which occurred during 2011 in Burgas Province, Bulgaria, a country that had been previously FMD-free-without-vaccination since 1996. Nineteen full genome sequences (FGS) of FMD virus (FMDV) were generated and analysed, including eight representative viruses from all of the virus-positive outbreaks of the disease in the country and 11 closely-related contemporary viruses from countries in the region where FMD is endemic (Turkey and Israel). All Bulgarian sequences shared a single putative common ancestor which was closely related to the index case identified in wild boar. The closest relative from outside of Bulgaria was a FMDV collected during 2010 in Bursa (Anatolia, Turkey). Within Bulgaria, two discrete genetic clusters were detected that corresponded to two episodes of outbreaks that occurred during January and March-April 2011. The number of nucleotide substitutions that were present between, and within, these separate clusters provided evidence that undetected FMDV infection had occurred. These conclusions are supported by laboratory data that subsequently identified three additional FMDV-infected livestock premises by serosurveillance, as well as a number of antibody positive wild boar on both sides of the border with Turkish Thrace. This study highlights how FGS analysis can be used as an effective on-the-spot tool to support and help direct epidemiological investigations of field outbreaks.

KW - Animals

KW - Base Sequence

KW - Bulgaria/epidemiology

KW - Cattle

KW - Cattle Diseases/epidemiology

KW - Disease Outbreaks

KW - Epidemiological Monitoring

KW - Evolution, Molecular

KW - Foot-and-Mouth Disease/epidemiology

KW - Foot-and-Mouth Disease Virus/genetics

KW - Genome, Viral

KW - Israel/epidemiology

KW - Multigene Family

KW - Phylogeny

KW - Phylogeography

KW - Sus scrofa

KW - Swine

KW - Swine Diseases/epidemiology

KW - Turkey/epidemiology

U2 - 10.1371/journal.pone.0049650

DO - 10.1371/journal.pone.0049650

M3 - Journal article

C2 - 23226216

VL - 7

SP - e49650

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 11

ER -

ID: 257916731