Raman mapping of mannitol/lysozyme particles produced via spray drying and single droplet drying
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Raman mapping of mannitol/lysozyme particles produced via spray drying and single droplet drying. / Pekka Pajander, Jari; Matero, Sanni Elina; Sloth, Jakob; Wan, Feng; Rantanen, Jukka; Yang, Mingshi.
In: Pharmaceutical Research, Vol. 32, No. 6, 2015, p. 1993-2002.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Raman mapping of mannitol/lysozyme particles produced via spray drying and single droplet drying
AU - Pekka Pajander, Jari
AU - Matero, Sanni Elina
AU - Sloth, Jakob
AU - Wan, Feng
AU - Rantanen, Jukka
AU - Yang, Mingshi
PY - 2015
Y1 - 2015
N2 - PURPOSE: This study aimed to investigate the effect of a model protein on the solid state of a commonly used bulk agent in spray-dried formulations.METHODS: A series of lysozyme/mannitol formulations were spray-dried using a lab-scale spray dryer. Further, the surface temperature of drying droplet/particles was monitored using the DRYING KINETICS ANALYZER™ (DKA) with controllable drying conditions mimicking the spray-drying process to estimate the drying kinetics of the lysozyme/mannitol formulations. The mannitol polymorphism and the spatial distribution of lysozyme in the particles were examined using X-ray powder diffractometry (XRPD) and Raman microscopy. Partial Least Squares Discriminant Analysis was used for analyzing the Raman microscopy data.RESULTS: XRPD results indicated that a mixture of β-mannitol and α-mannitol was produced in the spray-drying process which was supported by the Raman analysis, whereas Raman analysis indicated that a mixture of α-mannitol and δ-mannitol was detected in the single particles from DKA. In addition Raman mapping indicated that the presence of lysozyme seemed to favor the appearance of α-mannitol in the particles from DKA evidenced by close proximity of lysozyme and mannitol in the particles.CONCLUSIONS: It suggested that the presence of lysozyme tend to induce metastable solid state forms upon the drying process.
AB - PURPOSE: This study aimed to investigate the effect of a model protein on the solid state of a commonly used bulk agent in spray-dried formulations.METHODS: A series of lysozyme/mannitol formulations were spray-dried using a lab-scale spray dryer. Further, the surface temperature of drying droplet/particles was monitored using the DRYING KINETICS ANALYZER™ (DKA) with controllable drying conditions mimicking the spray-drying process to estimate the drying kinetics of the lysozyme/mannitol formulations. The mannitol polymorphism and the spatial distribution of lysozyme in the particles were examined using X-ray powder diffractometry (XRPD) and Raman microscopy. Partial Least Squares Discriminant Analysis was used for analyzing the Raman microscopy data.RESULTS: XRPD results indicated that a mixture of β-mannitol and α-mannitol was produced in the spray-drying process which was supported by the Raman analysis, whereas Raman analysis indicated that a mixture of α-mannitol and δ-mannitol was detected in the single particles from DKA. In addition Raman mapping indicated that the presence of lysozyme seemed to favor the appearance of α-mannitol in the particles from DKA evidenced by close proximity of lysozyme and mannitol in the particles.CONCLUSIONS: It suggested that the presence of lysozyme tend to induce metastable solid state forms upon the drying process.
U2 - 10.1007/s11095-014-1592-z
DO - 10.1007/s11095-014-1592-z
M3 - Journal article
C2 - 25504535
VL - 32
SP - 1993
EP - 2002
JO - Pharmaceutical Research
JF - Pharmaceutical Research
SN - 0724-8741
IS - 6
ER -
ID: 162897994