Quantitative and Dynamic Catalogs of Proteins Released during Apoptotic and Necroptotic Cell Death

Research output: Contribution to journalJournal articleResearchpeer-review

  • Maria C Tanzer
  • Annika Frauenstein
  • Che A Stafford
  • Kshiti Phulphagar
  • Mann, Matthias
  • Felix Meissner

The inflammatory functions of the cytokine tumor necrosis factor (TNF) rely on its ability to induce cytokine production and to induce cell death. Caspase-dependent and caspase-independent pathways-apoptosis and necroptosis, respectively-regulate immunogenicity by the release of distinct sets of cellular proteins. To obtain an unbiased, systems-level understanding of this important process, we here applied mass spectrometry-based proteomics to dissect protein release during apoptosis and necroptosis. We report hundreds of proteins released from human myeloid cells in time course experiments. Both cell death types induce receptor shedding, but only apoptotic cells released nucleosome components. Conversely, necroptotic cells release lysosomal components by activating lysosomal exocytosis at early stages of necroptosis-induced membrane permeabilization and show reduced release of conventionally secreted cytokines.

Original languageEnglish
JournalCell Reports
Volume30
Issue number4
Pages (from-to)1260-1270.e5
ISSN2211-1247
DOIs
Publication statusPublished - 28 Jan 2020

Bibliographical note

Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.

ID: 239207159