Quantitative and Dynamic Catalogs of Proteins Released during Apoptotic and Necroptotic Cell Death

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Quantitative and Dynamic Catalogs of Proteins Released during Apoptotic and Necroptotic Cell Death. / Tanzer, Maria C; Frauenstein, Annika; Stafford, Che A; Phulphagar, Kshiti; Mann, Matthias; Meissner, Felix.

In: Cell Reports, Vol. 30, No. 4, 28.01.2020, p. 1260-1270.e5.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Tanzer, MC, Frauenstein, A, Stafford, CA, Phulphagar, K, Mann, M & Meissner, F 2020, 'Quantitative and Dynamic Catalogs of Proteins Released during Apoptotic and Necroptotic Cell Death', Cell Reports, vol. 30, no. 4, pp. 1260-1270.e5. https://doi.org/10.1016/j.celrep.2019.12.079

APA

Tanzer, M. C., Frauenstein, A., Stafford, C. A., Phulphagar, K., Mann, M., & Meissner, F. (2020). Quantitative and Dynamic Catalogs of Proteins Released during Apoptotic and Necroptotic Cell Death. Cell Reports, 30(4), 1260-1270.e5. https://doi.org/10.1016/j.celrep.2019.12.079

Vancouver

Tanzer MC, Frauenstein A, Stafford CA, Phulphagar K, Mann M, Meissner F. Quantitative and Dynamic Catalogs of Proteins Released during Apoptotic and Necroptotic Cell Death. Cell Reports. 2020 Jan 28;30(4):1260-1270.e5. https://doi.org/10.1016/j.celrep.2019.12.079

Author

Tanzer, Maria C ; Frauenstein, Annika ; Stafford, Che A ; Phulphagar, Kshiti ; Mann, Matthias ; Meissner, Felix. / Quantitative and Dynamic Catalogs of Proteins Released during Apoptotic and Necroptotic Cell Death. In: Cell Reports. 2020 ; Vol. 30, No. 4. pp. 1260-1270.e5.

Bibtex

@article{f9f29f9f6b8e4787acaa515c2dd1d84b,

title = "Quantitative and Dynamic Catalogs of Proteins Released during Apoptotic and Necroptotic Cell Death",

abstract = "The inflammatory functions of the cytokine tumor necrosis factor (TNF) rely on its ability to induce cytokine production and to induce cell death. Caspase-dependent and caspase-independent pathways-apoptosis and necroptosis, respectively-regulate immunogenicity by the release of distinct sets of cellular proteins. To obtain an unbiased, systems-level understanding of this important process, we here applied mass spectrometry-based proteomics to dissect protein release during apoptosis and necroptosis. We report hundreds of proteins released from human myeloid cells in time course experiments. Both cell death types induce receptor shedding, but only apoptotic cells released nucleosome components. Conversely, necroptotic cells release lysosomal components by activating lysosomal exocytosis at early stages of necroptosis-induced membrane permeabilization and show reduced release of conventionally secreted cytokines.",

author = "Tanzer, {Maria C} and Annika Frauenstein and Stafford, {Che A} and Kshiti Phulphagar and Matthias Mann and Felix Meissner",

year = "2020",

month = jan,

day = "28",

doi = "10.1016/j.celrep.2019.12.079",

language = "English",

volume = "30",

pages = "1260--1270.e5",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "4",

}

RIS

TY - JOUR

T1 - Quantitative and Dynamic Catalogs of Proteins Released during Apoptotic and Necroptotic Cell Death

AU - Tanzer, Maria C

AU - Frauenstein, Annika

AU - Stafford, Che A

AU - Phulphagar, Kshiti

AU - Mann, Matthias

AU - Meissner, Felix

PY - 2020/1/28

Y1 - 2020/1/28

N2 - The inflammatory functions of the cytokine tumor necrosis factor (TNF) rely on its ability to induce cytokine production and to induce cell death. Caspase-dependent and caspase-independent pathways-apoptosis and necroptosis, respectively-regulate immunogenicity by the release of distinct sets of cellular proteins. To obtain an unbiased, systems-level understanding of this important process, we here applied mass spectrometry-based proteomics to dissect protein release during apoptosis and necroptosis. We report hundreds of proteins released from human myeloid cells in time course experiments. Both cell death types induce receptor shedding, but only apoptotic cells released nucleosome components. Conversely, necroptotic cells release lysosomal components by activating lysosomal exocytosis at early stages of necroptosis-induced membrane permeabilization and show reduced release of conventionally secreted cytokines.

AB - The inflammatory functions of the cytokine tumor necrosis factor (TNF) rely on its ability to induce cytokine production and to induce cell death. Caspase-dependent and caspase-independent pathways-apoptosis and necroptosis, respectively-regulate immunogenicity by the release of distinct sets of cellular proteins. To obtain an unbiased, systems-level understanding of this important process, we here applied mass spectrometry-based proteomics to dissect protein release during apoptosis and necroptosis. We report hundreds of proteins released from human myeloid cells in time course experiments. Both cell death types induce receptor shedding, but only apoptotic cells released nucleosome components. Conversely, necroptotic cells release lysosomal components by activating lysosomal exocytosis at early stages of necroptosis-induced membrane permeabilization and show reduced release of conventionally secreted cytokines.

U2 - 10.1016/j.celrep.2019.12.079

DO - 10.1016/j.celrep.2019.12.079

M3 - Journal article

C2 - 31995763

VL - 30

SP - 1260-1270.e5

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 4

ER -

ID: 239207159