Quantification of specific E. coli in gut mucosa from Crohn's disease patients
Research output: Contribution to journal › Journal article › Research › peer-review
We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.
|Journal||Journal of Microbiological Methods|
|Number of pages||4|
|Publication status||Published - Jul 2011|
- Crohn Disease, DNA Primers, Escherichia coli, Humans, Intestinal Mucosa, Polymerase Chain Reaction, Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't