Quantification of specific E. coli in gut mucosa from Crohn's disease patients

Research output: Contribution to journalJournal articleResearchpeer-review

We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.

Original languageEnglish
JournalJournal of Microbiological Methods
Issue number1
Pages (from-to)111-4
Number of pages4
Publication statusPublished - Jul 2011

    Research areas

  • Crohn Disease, DNA Primers, Escherichia coli, Humans, Intestinal Mucosa, Polymerase Chain Reaction, Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't

ID: 166456201