Quantification of protein modification by oxidants
Research output: Contribution to journal › Journal article › peer-review
Proteins are major targets for oxidative damage due to their abundance and rapid rates of reaction with a wide range of radicals and excited state species, such as singlet oxygen. Exposure of proteins to these oxidants results in loss of the parent amino acid residue, formation of unstable intermediates, and the generation of stable products. Each of these events can be used, to a greater or lesser extent, to quantify damage to proteins. In this review the advantages and disadvantages of a number of these approaches are discussed, with an emphasis on methods that yield absolute quantitative data on the extent of protein modification. Detailed methods sheets are provided for many of these techniques.
|Journal||Free Radical Biology & Medicine|
|Number of pages||24|
|Publication status||Published - 15 Apr 2009|
- Animals, Biochemistry, Biological Markers, Chromatography, High Pressure Liquid, Enzyme-Linked Immunosorbent Assay, Humans, Mass Spectrometry, Oxidants, Oxidation-Reduction, Oxidative Stress, Protein Processing, Post-Translational, Proteins, Reactive Oxygen Species, Spin Trapping, Statistics as Topic