Pseudomonas aeruginosa recognizes and responds aggressively to the presence of polymorphonuclear leukocytes
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Pseudomonas aeruginosa recognizes and responds aggressively to the presence of polymorphonuclear leukocytes. / Alhede, Morten; Bjarnsholt, Thomas; Jensen, Peter Østrup; Phipps, Richard Kerry; Moser, Claus; Christophersen, Lars; Hultqvist, Louise Dahl; van Gennip, Maria; Parsek, Matt; Høiby, Niels; Rasmussen, Thomas Bovbjerg; Givskov, Michael.
In: Microbiology, Vol. 155, No. 11, 2009, p. 3500-3508.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Pseudomonas aeruginosa recognizes and responds aggressively to the presence of polymorphonuclear leukocytes
AU - Alhede, Morten
AU - Bjarnsholt, Thomas
AU - Jensen, Peter Østrup
AU - Phipps, Richard Kerry
AU - Moser, Claus
AU - Christophersen, Lars
AU - Hultqvist, Louise Dahl
AU - van Gennip, Maria
AU - Parsek, Matt
AU - Høiby, Niels
AU - Rasmussen, Thomas Bovbjerg
AU - Givskov, Michael
PY - 2009
Y1 - 2009
N2 - Polymorphonuclear neutrophilic leukocytes (PMNs) play a central role in innate immunity, where they dominate the response to infections, in particular in the cystic fibrosis lung. PMNs are phagocytic cells that produce a wide range of antimicrobial agents aimed at killing invading bacteria. However, the opportunistic pathogen Pseudomonas aeruginosa can evade destruction by PMNs and thus cause persistent infections. In this study, we show that biofilm cells of P. aeruginosa recognize the presence of attracted PMNs and direct this information to their fellow bacteria through the quorum sensing (QS) signalling system. The bacteria respond to the presence of PMNs by upregulating synthesis of a number of QS-controlled virulence determinants including rhamnolipids, all of which are able to cripple and eliminate cells of the host defence. Our in vitro and in vivo analyses support a 'launch a shield' model by which rhamnolipids surround the biofilm bacteria and on contact eliminate incoming PMNs. Our data strengthen the view that cross-kingdom communication plays a key role in P. aeruginosa recognition and evasion of the host defence.
AB - Polymorphonuclear neutrophilic leukocytes (PMNs) play a central role in innate immunity, where they dominate the response to infections, in particular in the cystic fibrosis lung. PMNs are phagocytic cells that produce a wide range of antimicrobial agents aimed at killing invading bacteria. However, the opportunistic pathogen Pseudomonas aeruginosa can evade destruction by PMNs and thus cause persistent infections. In this study, we show that biofilm cells of P. aeruginosa recognize the presence of attracted PMNs and direct this information to their fellow bacteria through the quorum sensing (QS) signalling system. The bacteria respond to the presence of PMNs by upregulating synthesis of a number of QS-controlled virulence determinants including rhamnolipids, all of which are able to cripple and eliminate cells of the host defence. Our in vitro and in vivo analyses support a 'launch a shield' model by which rhamnolipids surround the biofilm bacteria and on contact eliminate incoming PMNs. Our data strengthen the view that cross-kingdom communication plays a key role in P. aeruginosa recognition and evasion of the host defence.
KW - Animals
KW - Biofilms
KW - Bronchoalveolar Lavage Fluid
KW - DNA, Bacterial
KW - Dynorphins
KW - Female
KW - Gene Expression Regulation, Bacterial
KW - Glycolipids
KW - Lung Diseases
KW - Mice
KW - Mice, Inbred BALB C
KW - Neutrophils
KW - Oligonucleotide Array Sequence Analysis
KW - Pseudomonas Infections
KW - Pseudomonas aeruginosa
KW - Quorum Sensing
KW - Virulence
U2 - 10.1099/mic.0.031443-0
DO - 10.1099/mic.0.031443-0
M3 - Journal article
C2 - 19643762
VL - 155
SP - 3500
EP - 3508
JO - Microbiology
JF - Microbiology
SN - 1350-0872
IS - 11
ER -
ID: 105878000