Protoplast Isolation and Transfection in Wheat

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Protoplast Isolation and Transfection in Wheat. / Luo, Guangbin; Li, Boshu; Gao, Caixia.

Protoplast Technology : Methods and Protocols. ed. / Kan Wang; Feng Zhang. Springer, 2022. p. 131-141 (Methods in Molecular Biology, Vol. 2464).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Luo, G, Li, B & Gao, C 2022, Protoplast Isolation and Transfection in Wheat. in K Wang & F Zhang (eds), Protoplast Technology : Methods and Protocols. Springer, Methods in Molecular Biology, vol. 2464, pp. 131-141. https://doi.org/10.1007/978-1-0716-2164-6_10

APA

Luo, G., Li, B., & Gao, C. (2022). Protoplast Isolation and Transfection in Wheat. In K. Wang, & F. Zhang (Eds.), Protoplast Technology : Methods and Protocols (pp. 131-141). Springer. Methods in Molecular Biology Vol. 2464 https://doi.org/10.1007/978-1-0716-2164-6_10

Vancouver

Luo G, Li B, Gao C. Protoplast Isolation and Transfection in Wheat. In Wang K, Zhang F, editors, Protoplast Technology : Methods and Protocols. Springer. 2022. p. 131-141. (Methods in Molecular Biology, Vol. 2464). https://doi.org/10.1007/978-1-0716-2164-6_10

Author

Luo, Guangbin ; Li, Boshu ; Gao, Caixia. / Protoplast Isolation and Transfection in Wheat. Protoplast Technology : Methods and Protocols. editor / Kan Wang ; Feng Zhang. Springer, 2022. pp. 131-141 (Methods in Molecular Biology, Vol. 2464).

Bibtex

@inbook{76a85cf4c40f41338135da4ecb919a27,

title = "Protoplast Isolation and Transfection in Wheat",

abstract = "Wheat is one of the major staple crops around the world. A transient expression system is crucial for gene functional studies in wheat as stable transfection is still difficult in most cultivars. Protoplasts could serve as a versatile transient expression tool in wheat research. Here, we describe protocols for wheat protoplast isolation and transfection that are enabled by cellulase R-10 and macerozyme R-10 containing enzymatic solution and polyethylene glycol-mediated method, respectively. In addition, we show an example of efficiency evaluation of the emerging base editors in wheat protoplasts. These protocols are of wide use in both conventional gene functional analysis and reagent functionality evaluation of genome editing in wheat.",

keywords = "Base editor, Base-editing efficiency, Genome editing, Polyethylene glycol (PEG), Triticum aestivum",

author = "Guangbin Luo and Boshu Li and Caixia Gao",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

doi = "10.1007/978-1-0716-2164-6_10",

language = "English",

series = "Methods in Molecular Biology",

publisher = "Springer",

pages = "131--141",

editor = "Kan Wang and Feng Zhang",

booktitle = "Protoplast Technology",

address = "Switzerland",

}

RIS

TY - CHAP

T1 - Protoplast Isolation and Transfection in Wheat

AU - Luo, Guangbin

AU - Li, Boshu

AU - Gao, Caixia

PY - 2022

Y1 - 2022

N2 - Wheat is one of the major staple crops around the world. A transient expression system is crucial for gene functional studies in wheat as stable transfection is still difficult in most cultivars. Protoplasts could serve as a versatile transient expression tool in wheat research. Here, we describe protocols for wheat protoplast isolation and transfection that are enabled by cellulase R-10 and macerozyme R-10 containing enzymatic solution and polyethylene glycol-mediated method, respectively. In addition, we show an example of efficiency evaluation of the emerging base editors in wheat protoplasts. These protocols are of wide use in both conventional gene functional analysis and reagent functionality evaluation of genome editing in wheat.

AB - Wheat is one of the major staple crops around the world. A transient expression system is crucial for gene functional studies in wheat as stable transfection is still difficult in most cultivars. Protoplasts could serve as a versatile transient expression tool in wheat research. Here, we describe protocols for wheat protoplast isolation and transfection that are enabled by cellulase R-10 and macerozyme R-10 containing enzymatic solution and polyethylene glycol-mediated method, respectively. In addition, we show an example of efficiency evaluation of the emerging base editors in wheat protoplasts. These protocols are of wide use in both conventional gene functional analysis and reagent functionality evaluation of genome editing in wheat.

KW - Base editor

KW - Base-editing efficiency

KW - Genome editing

KW - Polyethylene glycol (PEG)

KW - Triticum aestivum

U2 - 10.1007/978-1-0716-2164-6_10

DO - 10.1007/978-1-0716-2164-6_10

M3 - Book chapter

C2 - 35258830

AN - SCOPUS:85125971867

T3 - Methods in Molecular Biology

SP - 131

EP - 141

BT - Protoplast Technology

A2 - Wang, Kan

A2 - Zhang, Feng

PB - Springer

ER -

ID: 337988857