Proteomic changes in Debaryomyces hansenii upon exposure to NaCl stress
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Proteomic changes in Debaryomyces hansenii upon exposure to NaCl stress. / Gori, Klaus; Hébraud, Michel; Chambon, Christophe; Mortensen, Henrik Dam; Arneborg, Nils; Jespersen, Lene.
In: FEMS Yeast Research, Vol. 7, No. 2, 2007, p. 293-303.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Proteomic changes in Debaryomyces hansenii upon exposure to NaCl stress
AU - Gori, Klaus
AU - Hébraud, Michel
AU - Chambon, Christophe
AU - Mortensen, Henrik Dam
AU - Arneborg, Nils
AU - Jespersen, Lene
PY - 2007
Y1 - 2007
N2 - The proteome of the highly NaCl-tolerant yeast Debaryomyces hansenii was investigated by two-dimensional polyacrylamide gel electrophoresis (2D PAGE), and 47 protein spots were identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) followed by mass spectrometry (MS). The influence of NaCl on the D. hansenii proteome was investigated during the first 3 h of NaCl exposure. The rate of protein synthesis was strongly decreased by exposure to 8% and 12% (w/v) NaCl, as the average incorporation rates of l-[(35)S]methionine within the first 30 min after addition of NaCl were only 7% and 4% of the rate in medium without NaCl. In addition, the number of protein spots detected on 2D gels prepared from cells exposed to 8% and 12% (w/v) NaCl exceeded less than 28% of the number of protein spots detected on 2D gels prepared from cells without added NaCl. Several proteins were identified as being either induced or repressed upon NaCl exposure. The induced proteins were enzymes involved in glycerol synthesis/dissimilation and the upper part of glycolysis, whereas the repressed proteins were enzymes involved in the lower part of glycolysis, the route to the Krebs cycle, and the synthesis of amino acids. Furthermore, one heat shock protein (Ssa1p) was induced, whereas others (Ssb2p and Hsp60p) were repressed.
AB - The proteome of the highly NaCl-tolerant yeast Debaryomyces hansenii was investigated by two-dimensional polyacrylamide gel electrophoresis (2D PAGE), and 47 protein spots were identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) followed by mass spectrometry (MS). The influence of NaCl on the D. hansenii proteome was investigated during the first 3 h of NaCl exposure. The rate of protein synthesis was strongly decreased by exposure to 8% and 12% (w/v) NaCl, as the average incorporation rates of l-[(35)S]methionine within the first 30 min after addition of NaCl were only 7% and 4% of the rate in medium without NaCl. In addition, the number of protein spots detected on 2D gels prepared from cells exposed to 8% and 12% (w/v) NaCl exceeded less than 28% of the number of protein spots detected on 2D gels prepared from cells without added NaCl. Several proteins were identified as being either induced or repressed upon NaCl exposure. The induced proteins were enzymes involved in glycerol synthesis/dissimilation and the upper part of glycolysis, whereas the repressed proteins were enzymes involved in the lower part of glycolysis, the route to the Krebs cycle, and the synthesis of amino acids. Furthermore, one heat shock protein (Ssa1p) was induced, whereas others (Ssb2p and Hsp60p) were repressed.
KW - Former LIFE faculty
KW - Debaryomyces hansenii; proteome; 2D PAGE; NaCl stress
U2 - 10.1111/j.1567-1364.2006.00155.x
DO - 10.1111/j.1567-1364.2006.00155.x
M3 - Journal article
C2 - 17328743
VL - 7
SP - 293
EP - 303
JO - FEMS Yeast Research
JF - FEMS Yeast Research
SN - 1567-1356
IS - 2
ER -
ID: 8108479