Proteomic approaches in circadian biology

Research output: Chapter in Book/Report/Conference proceedingBook chapterCommunication

Circadian clocks are endogenous oscillators that drive the rhythmic expression of a broad array of genes that orchestrate metabolism and physiology. Recent evidence indicates that posttranscriptional and posttranslational mechanisms play essential roles in modulating circadian gene expression, particularly for the molecular mechanism of the clock. In contrast to genetic technologies that have long been used to study circadian biology, proteomic approaches have so far been limited and, if applied at all, have used two-dimensional gel electrophoresis (2-DE). Here, we review the proteomics approaches applied to date in the circadian field, and we also discuss the exciting potential of using cutting-edge proteomics technology in circadian biology. Large-scale, quantitative protein abundance measurements will help to understand to what extent the circadian clock drives system wide rhythms of protein abundance downstream of transcription regulation.
Original languageEnglish
Title of host publicationHandbook of experimental pharmacology : Circadian Clocks
Number of pages19
Publication date2013
Pages389-407
DOIs
Publication statusPublished - 2013
Externally publishedYes
SeriesHandbook of Experimental Pharmacology
ISSN0171-2004

    Research areas

  • Animals, Brain, Circadian Clocks, Eye, Humans, Metabolomics, Proteomics, Suprachiasmatic Nucleus

ID: 88586811